Blood and sputum biomarkers in COPD and asthma: a review

Chronic obstructive pulmonary disease (COPD) and asthma are lung inflammatory diseases that represent major public health problems. The primary, and often unique, method to evaluate lung function is spirometry, which reflects disease severity rather than disease activity. Moreover, its measurements strictly depend on patient's compliance, physician's expertise and data interpretation. The limitations of clinical history and pulmonary function tests have encouraged focusing on new possible tracers of diseases. The increase of the inflammatory response in the lungs represents an early pathological event, so biological markers related to inflammation may play key roles in earlier diagnosis, evaluation of functional impairment and prognosis. Biomarkers are measurable indicators associated with the presence and/or severity of a biological or pathogenic process, which may predict functional impairment, prognosis and response to therapy. The traditional approach based on invasive techniques (bronchoalveolar lavage and biopsies) may be replaced, at least in part, by using less invasive methods to collect specimens (sputum and blood), in which biomarkers could be measured. Proteomics, by the association between different protein profiles and pathogenic processes, is gaining an important role in pulmonary medicine allowing a more precise discrimination between patients with different outcomes and response to therapy. The aim of this review was to evaluate the use of biomarkers of airway inflammation in the context of both research and clinical practice

Interview with Martha Pope, Abby Saffold and Marty Paone by Diane Dewhirst

Biographical NoteMartin Patrick “Marty” Paone was born in Everett, Massachusetts, in 1951. His father was a National Labor Relations Board field examiner and his mother was a nurse. He attended Boston College, graduating in 1972 with degrees in economics and philosophy. He moved to Washington, D.C. in September of 1974 to pursue a master’s degree in Russian studies at Georgetown University, and while there he worked in the House post office and as a parking lot attendant at the Senate parking lot. This led to a job in the Senate Cloakroom in 1979 after he completed his degree. In 1982 he joined the Democratic floor staff, and in 1991 he became assistant Democratic secretary of the Senate. In 1995 he succeeded Abby Saffold as the Democratic secretary and remained in that post until 2008. At the time of this interview, he was a member of the lobbying firm Timmons & Company. Martha Pope was born in Newcastle, Pennsylvania, and grew up in Connecticut. She attended the University of Connecticut, majoring in sociology with minors in psychology and statistics and in art. She earned a master’s degree in art education at Southern Connecticut University. She taught art for five years in elementary and junior high school, and then she moved to Washington, D.C. and started work on Capitol Hill. She worked for Senator John Culver, and when Culver lost his bid for reelection, Senator Mitchell kept her on as Environment and Public Works Committee staff focusing on fish and wildlife issues. She became his administrative assistant, and when he became majority leader she became chief of staff to the majority leader. In 1990 she was nominated to be sergeant-at-arms of the Senate, and in 1994 she became secretary of the Senate; she retired from that office in January 1995. She joined the State Department to work with Senator Mitchell on Northern Ireland issues, which eventually led to the Good Friday Peace Agreement of 1998. Abby Saffold was born Carol Abbott “Abby” Reid in Baltimore, Maryland, and attended high school in Framingham, Massachusetts. At Bates College, she majored in history with a minor in government, then began a master’s degree program for arts in teaching at Antioch College; as part of that program, she taught junior high school for a year in Washington, D.C. She decided to pursue a job on Capitol Hill and found work first for Congressman William Lloyd Scott and then Congressman Lloyd Meeds. Subsequently, she was hired as a legislative secretary by Senator Gaylord Nelson and then worked for the Subcommittee on Constitutional Amendments for Senator Birch Bayh. In 1979 she joined the Democratic Policy floor staff, where she remained until Senator Byrd nominated her to be secretary for the majority (Democratic secretary) in 1987. She retired from that position in 1995. SummaryInterview includes discussion of: how each of the interviewees came to work for Senator Mitchell; first impressions of Mitchell; Martha Pope’s work on the Environment and Public Works Committee; Mitchell’s intellectual capacity; Mitchell’s treatment of other senators and his staff; the impression that Mitchell made with Senator Byrd early on; Abby Saffold’s interaction with Mitchell as a member of the Democratic floor staff when he was a junior senator; majority leader race; Byrd’s parliamentarian skills; Mitchell’s speechmaking skills; Brunswick (Me.) bypass; authorization of boundaries for Acadia National Park; the reason Henry Kissinger was not asked to testify regarding Iran-Contra; Mitchell’s performance questioning Oliver North on Iran-Contra; an anecdote about Mitchell and Senator Cohen watching a basketball game together during the Iran-Contra affair; Mitchell’s relations with the Maine delegation; Mitchell’s leadership style; Mitchell’s relationship with Dole, the expectation that there would be no surprises; “read my lips, no new taxes” and President George H.W. Bush; the Clean Air Act reauthorization and tension with Byrd; Crime Bill; Senator Helms’s filibuster; the Clarence Thomas nomination and congresswomen marching on the Senate Democratic caucus; Marty Paone’s playing an April Fools joke on Mitchell; convincing Mitchell to do an interview with the National Journal before the leader race; and how Mitchell sparingly praised staff

Alexithymia and obesity: controversial findings from a multimethod assessment

OBJECTIVE: The aim of the study is to assess alexithymia levels in obese patients using a multimethod measurement (TAS-20 and TSIA) to evaluate both possible differences between the two instruments and their relationship with body weight. PATIENTS AND METHODS: 54 obese patients, seeking surgical treatment, were enrolled. They completed a socio-demographic questionnaire, 20-items Toronto Alexithymia Scale and the Toronto Structured Interview for Alexithymia. RESULTS: Data analysis showed a significant positive association between TAS-20 and TSIA total scores (r=.28, p<.05), but only the TSIA score was positively related to body weight (r=.39; p<.001). Multivariable linear regression models showed the predictive effects of TSIA total score (beta=.41; p<.001) and difficulty in identifying feelings (DIF) (beta=.56; p<.001) respectively on weight. CONCLUSIONS: The findings showed a different association between body weight and alexithymia according to the instrument employed to evaluate alexithymia, supporting the importance of a multimethod assessment in some clinical conditions

The Grizzly, February 18, 2010

Every Ending Starts with a Beginning • Record-Breaking Blizzard Evokes Varied Reactions • Could Watching the Super Bowl Damage Your Heart? • Snow Storm Photos • Senior Class Gift Drive • SPINTfest \u2710 Brings New Themes for Houses • UC Goes Red to Raise Awareness About the Risks of Heart Disease • Opinion: Teenage Pregnancy TV Shows are a Big Hit, But What\u27s the Effect? • Tragedy Strikes in Early Hours of Winter Olympics • Men\u27s Basketball Shuts Down McDanielhttps://digitalcommons.ursinus.edu/grizzlynews/1806/thumbnail.jp

Studying ggdef domain in the act: Minimize conformational frustration to prevent artefacts

GGDEF-containing proteins respond to different environmental cues to finely modulate cyclic diguanylate (c-di-GMP) levels in time and space, making the allosteric control a distinctive trait of the corresponding proteins. The diguanylate cyclase mechanism is emblematic of this control: two GGDEF domains, each binding one GTP molecule, must dimerize to enter catalysis and yield c-di-GMP. The need for dimerization makes the GGDEF domain an ideal conformational switch in multidomain proteins. A re-evaluation of the kinetic profile of previously characterized GGDEF domains indicated that they are also able to convert GTP to GMP: this unexpected reactivity occurs when conformational issues hamper the cyclase activity. These results create new questions regarding the characterization and engineering of these proteins for in solution or structural studies

Chapitre 5. Échanger, travailler, petites et grandes « industries » à Marseille

Quelques découvertes archéologiques particulières, au sein des quartiers d’habitation, nous renseignent sur des bâtiments liés au commerce et aux industries. C’est le cas en particulier d’un marché particulier, la boucherie, à proximité immédiate de l’axe principal que constitue la Grand-Rue. 1. Les marchés Deux marchés alimentaires importants ont pu être approchés par l’archéologie, plus exactement frôlés par des fouilles. Le premier, le marché du Petit Mazeau, marché à la viande, est au cœu..

The Grizzly, February 12, 2010

Main Street Renovation Improves Safety • Community Brings Down Crime at Ursinus College • Annual Scottish Irish Festival This Weekend • Bonner Leaders Hold Fair in Lower Wismer • What You Should Really Expect from Study Abroad • New Member Education Starts Up Again and Looks Forward to Positive Change • UC Gymnastics is Flipping Through 2010 Season • Moliken Named New Athletic Directorhttps://digitalcommons.ursinus.edu/grizzlynews/1805/thumbnail.jp

The Atlas of human African trypanosomiasis: a contribution to global mapping of neglected tropical diseases

<p>Abstract</p> <p>Background</p> <p>Following World Health Assembly resolutions 50.36 in 1997 and 56.7 in 2003, the World Health Organization (WHO) committed itself to supporting human African trypanosomiasis (HAT)-endemic countries in their efforts to remove the disease as a public health problem. Mapping the distribution of HAT in time and space has a pivotal role to play if this objective is to be met. For this reason WHO launched the HAT Atlas initiative, jointly implemented with the Food and Agriculture Organization of the United Nations, in the framework of the Programme Against African Trypanosomosis.</p> <p>Results</p> <p>The distribution of HAT is presented for 23 out of 25 sub-Saharan countries having reported on the status of sleeping sickness in the period 2000 - 2009. For the two remaining countries, i.e. Angola and the Democratic Republic of the Congo, data processing is ongoing. Reports by National Sleeping Sickness Control Programmes (NSSCPs), Non-Governmental Organizations (NGOs) and Research Institutes were collated and the relevant epidemiological data were entered in a database, thus incorporating (i) the results of active screening of over 2.2 million people, and (ii) cases detected in health care facilities engaged in passive surveillance. A total of over 42 000 cases of HAT and 6 000 different localities were included in the database. Various sources of geographic coordinates were used to locate the villages of epidemiological interest. The resulting average mapping accuracy is estimated at 900 m.</p> <p>Conclusions</p> <p>Full involvement of NSSCPs, NGOs and Research Institutes in building the Atlas of HAT contributes to the efficiency of the mapping process and it assures both the quality of the collated information and the accuracy of the outputs. Although efforts are still needed to reduce the number of undetected and unreported cases, the comprehensive, village-level mapping of HAT control activities over a ten-year period ensures a detailed and reliable representation of the known geographic distribution of the disease. Not only does the Atlas serve research and advocacy, but, more importantly, it provides crucial evidence and a valuable tool for making informed decisions to plan and monitor the control of sleeping sickness.</p

The Grizzly, April 8, 2010

First Annual Backwards Beauty Pageant Held • Ursinus Senior to Travel Abroad for a Year with Watson • Kappa Alpha Psi and Seismic Step Emerge on Campus • UC Welcomes New Field Hockey Coach • Sex on Wheels Documentary Screening • Volunteering with St. Christopher\u27s Children\u27s Hospital • Feeling Good in The Skin We\u27re In • The Sacrifice Your Body Makes for [Better] Grades • Opinion: Kyleigh\u27s Law Profiles Drivers by Age in New Jersey • Upper Classmen Off-Campus Living • UC Gymnastics Closes Season with Successhttps://digitalcommons.ursinus.edu/grizzlynews/1810/thumbnail.jp

Targeting the Interaction between the SH3 Domain of Grb2 and Gab2

Gab2 is a scaffolding protein, overexpressed in many types of cancers, that plays a key role in the formation of signaling complexes involved in cellular proliferation, migration, and differentiation. The interaction between Gab2 and the C-terminal SH3 domain of the protein Grb2 is crucial for the activation of the proliferation-signaling pathway Ras/Erk, thus representing a potential pharmacological target. In this study, we identified, by virtual screening, seven potential inhibitor molecules that were experimentally tested through kinetic and equilibrium binding experiments. One compound showed a remarkable effect in lowering the affinity of the C-SH3 domain for Gab2. This inhibitory effect was subsequently validated in cellula by using lung cancer cell lines A549 and H1299. Our results are discussed under the light of previous works on the C-SH3:Gab2 interaction