168 research outputs found
Chemical-Physical Properties and Bioactivity of New Premixed Calcium Silicate-Bioceramic Root Canal Sealers
The aim of the study was to analyze the chemical-physical properties and bioactivity (apatite-forming ability) of three recently introduced premixed bioceramic root canal sealers containing varied amounts of different calcium silicates (CaSi): a dicalcium and tricalcium silicate (1-10% and 20-30%)-containing sealer with zirconium dioxide and tricalcium aluminate (CERASEAL); a tricalcium silicate (5-15%)-containing sealer with zirconium dioxide, dimethyl sulfoxide and lithium carbonate (AH PLUS BIOCERAMIC) and a dicalcium and tricalcium silicate (10% and 25%)-containing sealer with calcium aluminate, tricalcium aluminate and tantalite (NEOSEALER FLO). An epoxy resin-based sealer (AH PLUS) was used as control. The initial and final setting times, radiopacity, flowability, film thickness, open pore volume, water absorption, solubility, calcium release and alkalizing activity were tested. The nucleation of calcium phosphates and/or apatite after 28 days aging in Hanks balanced salt solution (HBSS) was evaluated by ESEM-EDX, vibrational IR and micro-Raman spectroscopy. The analyses showed for NeoSealer Flo and AH Plus the longest final setting times (1344 +/- 60 and 1300 +/- 60 min, respectively), while shorter times for AH Plus Bioceramic and Ceraseal (660 +/- 60 and 720 +/- 60 min, respectively). Radiopacity, flowability and film thickness complied with ISO 6876/12 for all tested materials. A significantly higher open pore volume was observed for NeoSealer Flo, AH Plus Bioceramic and Ceraseal when compared to AH Plus (p < 0.05), significantly higher values were observed for NeoSealer Flo and AH Plus Bioceramic (p < 0.05). Ceraseal and AH Plus revealed the lowest solubility. All CaSi-containing sealers released calcium and alkalized the soaking water. After 28 days immersion in HBSS, ESEM-EDX analyses revealed the formation of a mineral layer that covered the surface of all bioceramic sealers, with a lower detection of radiopacifiers (Zirconium for Ceraseal and AH Plus Bioceramic, Tantalum for NeoSealer Flo) and an increase in calcium, phosphorous and carbon. The calcium phosphate (CaP) layer was more evident on NeoSealer Flo and AH Plus Bioceramic. IR and micro-Raman revealed the formation of calcium carbonate on the surface of all set materials. A thin layer of a CaP phase was detected only on AH Plus Bioceramic and NeoSealer Flo. Ceraseal did not show CaP deposit despite its highest calcium release among all the tested CaSi-containing sealers. In conclusion, CaSi-containing sealers met the required chemical and physical standards and released biologically relevant ions. Slight/limited apatite nucleation was observed in relation to the high carbonation processes
Occurrence of Bifidobacteriaceae in human hypochlorhydria stomach
open7noBackground: The human stomach, when healthy, is not a suitable host for microorganisms, but in pathological
conditions such as gastritis, when gastric acid secretion is impaired, microbial overgrowth can be observed.
Apart from Helicobacter pylori, the composition of microbiota, resident or exogenously introduced during
neutral/high pH conditions, has not been investigated thoroughly. Thus, it is possible that Bifidobacteriaceae,
important autochthonous and beneficial bacteria of human gastrointestinal microbiota, could over-colonize
the stomach of hypochlorhydria patients suffering from autoimmune atrophic gastritis (AAG) or omeprazoletreated
(OME) gastritis. This prompted us to characterize the Bifidobacteriaceae in such patients’ gastric
microbiota and to study its abnormal colonization.
Methods: Samples of gastric juices, and antrum and corpus mucosa from 23 hypochlorhydria patients (13
AAG and 10 OME) and from 10 control volunteers with base-line normochlorhydria, were cultivated in Brain
Heart Infusion (BHI) and selective Bifidobacterium-Tryptone-Phytone-Yeast extract (Bif-TPY) media. The
isolates were characterized by the fructose-6-phosphate phosphoketolase (F6PPK) test, electrophoresis of
cellular proteins, the fermentation test, guanine-cytosine% DNA content, and DNADNA hybridization.
Negative F6PPK isolates were characterized by order-specific polymerase chain reaction (PCR).
Results: A total of 125 isolates, assigned to the Bifidobacteriaceae family on the basis of their morphology,
were obtained from AAG and OME patients, but not from normal subjects. Of these isolates, 55 were
assigned to the Bifidobacteriaceae family on the basis of their fructose-6-phosphoketolase (PPK) activity,
PPK being the key taxonomic enzyme of this family. The remaining 70 isolates, which were PPK-negative,
were attributed to the Actinomycetales order following specific primer PCR analysis. We observed a
significantly higher abundance of Bifidobacteriaceae (Bifidobacterium dentium, Scardovia inopinata, and
Parascardovia denticolens) in OME group than the AAG group. Furthermore, the Actinomycetales
distribution was homogeneous for both hypochlorhydria patient groups.
Conclusions: This study suggests that the Bifidobacteriaceae species, typically found in the oral cavity, readily
colonizes the hypochlorhydria stomach of OME patients. The clinical relevance and the mechanism
underlying this Bifidobacteriaceae presence in OME gastritis requires further functional studies.openPaola Mattarelli; Giovanni Brandi; Carlo Calabrese; Fabio Fornari; Gian Maria Prati; Bruno Biavati; Barbara SgorbatiPaola Mattarelli; Giovanni Brandi; Carlo Calabrese; Fabio Fornari; Gian Maria Prati; Bruno Biavati; Barbara Sgorbat
Green Hydrogels Composed of Sodium Mannuronate/Guluronate, Gelatin and Biointeractive Calcium Silicates/Dicalcium Phosphate Dihydrate Designed for Oral Bone Defects Regeneration
Innovative green, eco-friendly, and biologically derived hydrogels for non-load bearing bone sites were conceived and produced. Natural polysaccharides (copolymers of sodium
D-mannuronate and L-guluronate) with natural polypeptides (gelatin) and bioactive mineral fillers
(calcium silicates CaSi and dicalcium phosphate dihydrate DCPD) were used to obtain eco-sustainable
biomaterials for oral bone defects. Three PP-x:y formulations were prepared (PP-16:16, PP-33:22,
and PP-31:31), where PP represents the polysaccharide/polypeptide matrix and x and y represent
the weight % of CaSi and DCPD, respectively. Hydrogels were tested for their chemical-physical
properties (calcium release and alkalizing activity in deionized water, porosity, solubility, water
sorption, radiopacity), surface microchemistry and micromorphology, apatite nucleation in HBSS by
ESEM-EDX, FT-Raman, and micro-Raman spectroscopies. The expression of vascular (CD31) and
osteogenic (alkaline phosphatase ALP and osteocalcin OCN) markers by mesenchymal stem cells
(MSCs) derived from human vascular walls, cultured in direct contact with hydrogels or with 10% of
extracts was analysed. All mineral-filled hydrogels, in particular PP-31:31 and PP-33:22, released
Calcium ions and alkalized the soaking water for three days. Calcium ion leakage was high at all the
endpoints (3 h–28 d), while pH values were high at 3 h–3 d and then significantly decreased after
seven days (p < 0.05). Porosity, solubility, and water sorption were higher for PP-31:31 (p < 0.05).
The ESEM of fresh samples showed a compact structure with a few pores containing small mineral
granules agglomerated in some areas (size 5–20 microns). PP-CTRL degraded after 1–2 weeks in
HBSS. EDX spectroscopy revealed constitutional compounds and elements of the hydrogel (C, O, N,
and S) and of the mineral powders (Ca, Si and P). After 28 days in HBSS, the mineral-filled hydrogels revealed a more porous structure, partially covered with a thicker mineral layer on PP-31:31.
EDX analyses of the mineral coating showed Ca and P, and Raman revealed the presence of B-type
carbonated apatite and calcite. MSCs cultured in contact with mineral-filled hydrogels revealed the
expression of genes related to vascular (CD31) and osteogenic (mainly OCN) differentiation. Lower
gene expression was found when cells were cultured with extracts added to the culture medium. The
incorporation of biointeractive mineral powders in a green bio-derived algae-based matrix allowed
to produce bioactive porous hydrogels able to release biologically relevant ions and create a suitable
micro-environment for stem cells, resulting in interesting materials for bone regeneration and healing
in oral bone defects
Boron-loaded liposomes in the treatment of hepatic metastases: preliminary investigation by autoradiography analysis.
Boronophenylalanine (BPA)-loaded conventional and stabilized liposomes were prepared by the reversed phase evaporation method to treat liver metastases by boron neutron capture therapy. Conventional vesicles were composed of phosphatidylcholine and cholesterol, molar ratio 1:1. To obtain stealth liposomes, GM1 or PEG were included in the lipidic bilayer at a concentration of 6.67 or 5 mol%, respectively. Large unilamellar vesicles were formulated encapsulating BPA in the liposome aqueous compartment as a complex with fructose; BPA free base also was embedded into the lipidic bilayer. In vivo experiments were carried out after intravenous injection of liposome suspensions in BD-IX strain rats in which liver metastases had been induced. Alpha particle spectroscopy associated with histological analysis was performed to visualize boron spatial distribution in liver. Simultaneously, tissue boron concentrations were determined using inductively coupled plasma-mass spectroscopy. Results showed that PEG-modified liposomes accumulated boron in therapeutic concentrations (30 micrograms boron/g tissue) in metastatic tissue. The PEG-liposomes could be further explored in enhancing boron delivery to tumor cells
Gut microbiota profiles and characterization of cultivable fungal isolates in IBS patients
Studies so far conducted on irritable bowel syndrome (IBS) have been focused mainly on the role of gut bacterial dysbiosis in modulating the intestinal permeability, inflammation, and motility, with consequences on the quality of life. Limited evidences showed a potential involvement of gut fungal communities. Here, the gut bacterial and fungal microbiota of a cohort of IBS patients have been characterized and compared with that of healthy subjects (HS). The IBS microbial community structure differed significantly compared to HS. In particular, we observed an enrichment of bacterial taxa involved in gut inflammation, such as Enterobacteriaceae, Streptococcus, Fusobacteria, Gemella, and Rothia, as well as depletion of health-promoting bacterial genera, such as Roseburia and Faecalibacterium. Gut microbial profiles in IBS patients differed also in accordance with constipation. Sequence analysis of the gut mycobiota showed enrichment of Saccharomycetes in IBS. Culturomics analysis of fungal isolates from feces showed enrichment of Candida spp. displaying from IBS a clonal expansion and a distinct genotypic profiles and different phenotypical features when compared to HS of Candida albicans isolates. Alongside the well-characterized gut bacterial dysbiosis in IBS, this study shed light on a yet poorly explored fungal component of the intestinal ecosystem, the gut mycobiota. Our results showed a differential fungal community in IBS compared to HS, suggesting potential for new insights on the involvement of the gut mycobiota in IBS. KEY POINTS: Comparison of gut microbiota and mycobiota between IBS and healthy subjects Investigation of cultivable fungi in IBS and healthy subjects Candida albicans isolates result more virulent in IBS subjects compared to healthy subjects
A pilot study of the role of corn dextrin and milk peptides supplementation on faecal microbiota in healthy adults
The gastrointestinal microbiota has an important role in human health. Dietary interventions are of great interest to modulate the
composition and metabolic functions of the gut microbial communities and to improve health, and prevent or treat diseases. Consumption
of prebiotics is one dietary strategy for beneficial manipulation of the gut microbiota, because it allows increasing the
fibre intake, especially in people with western dietary habits, who do not take the recommended daily amount of fiber. Interestingly,
milk peptides can also positively affect the beneficial gut microorganisms. The present work is a pilot study aimed to investigate
the effect of a prebiotic supplementation on composition and metabolic activity of microorganisms living in the human gut. In
this trial, 12 healthy subjects received 10g/die of supplement Biotransit\uae, composed by corn derived dextrin and milk peptides,
produced and marketed in Italy by Depofarma (Italy), for 4 weeks with a 2 weeks washout. Outcome measures were assessed
at four time points (before the supplementation T0-1, T0-2, at the end of intervention, T30 and after washout, T45), including
gut microbiota profiling by 16S rRNA gene sequencing and intestinal functional metabolism measuring faecal Short Chain Fatty
Acid concentrations (SCFAs). The effects of the Biotransit supplementation on bifidobacteria were also assessed with culture dependent
techniques. Gut microbiota analysis revealed that Biotransit\uae supplementation after 30 days did not exert effects on the
overall gut microbiota structure. Although no significant differences on alpha diversity were obtained, we observed an increase of
diversity after 30 days of treatment. Beta diversity analysis, calculated on Bray-Curtis distances revealed significant differences
comparing T0 vs T45 and T30 vs T45. Interestingly, at T45, we found an enrichment of Porphyromonadaceae. Biotransit\uae induced
quantitative changes in cultivable bifidobacteria with increased amount at T45, even if the total number of species has not been
influenced. Biotransit\uae supplementation is also associated to an increase total SCFAs concentration in T30 and T45, in particular
related to acetate, propionate and butyrate (p < 0.05). Future study will be aimed to follow the time course of the persistence of
this effect after the end of treatment
Colorimetric analysis of painting materials using polymer-supported polydiacetylene films
Polydiacetylenes, exhibiting different headgroups, distinguish among different painting materials
A new bio-based organogel for the removal of wax coating from indoor bronze surfaces
Abstract In this research, we propose an advanced system for the cleaning of wax-based coatings applied on indoor bronzes. To this aim we developed a new kind of eco-friendly gel based on PHB (poly-3-hydroxybutyrate) used as thickening agent, biodiesel (BD) and dimethyl carbonate (DMC). BD is a mixture of methyl esters obtained from palm oil, which acts as cleaning agent while DMC was added as additional solvent to partially solubilize PHB and forming a gelly phase. For the first time a PHB-based gel obtained by mixing two solvents with different proprieties was proposed, expanding the range of possible formulations, that can be used according to the specific restoration purpose. After the preliminary characterization of chemical and physical properties of the gel, an ad hoc analytical protocol was implemented to evaluate both the cleaning efficiency and the release of residues on the treated surfaces. Standard samples were prepared following ancient recipes and submitted to spectroscopic and chromatographic analysis before and after the cleaning procedures. Finally, the performances of PHB-DMC/BD gel were assessed on a real case of study presenting a wax-based coating: the Pulpito della passione attributed to Donatello and dated back to 1460. In situ analysis demonstrated the high cleaning efficiency of the proposed systems also for the removal of aged coatings
22Ne and 23Na ejecta from intermediate-mass stars: The impact of the new LUNA rate for 22Ne(p,gamma)23Na
We investigate the impact of the new LUNA rate for the nuclear reaction NeNa on the chemical ejecta of intermediate-mass stars, with particular focus on the thermally-pulsing asymptotic giant branch (TP-AGB) stars that experience hot-bottom burning. To this aim we use the PARSEC and COLIBRI codes to compute the complete evolution, from the pre-main sequence up to the termination of the TP-AGB phase, of a set of stellar models with initial masses in the range , and metallicities , , and . We find that the new LUNA measures have much reduced the nuclear uncertainties of the Ne and Na AGB ejecta, which drop from factors of to only a factor of few for the lowest metallicity models. Relying on the most recent estimations for the destruction rate of Na, the uncertainties that still affect the Ne and Na AGB ejecta are mainly dominated by evolutionary aspects (efficiency of mass-loss, third dredge-up, convection). Finally, we discuss how the LUNA results impact on the hypothesis that invokes massive AGB stars as the main agents of the observed O-Na anti-correlation in Galactic globular clusters. We derive quantitative indications on the efficiencies of key physical processes (mass loss, third dredge-up, sodium destruction) in order to simultaneously reproduce both the Na-rich, O-poor extreme of the anti-correlation, and the observational constraints on the CNO abundance. Results for the corresponding chemical ejecta are made publicly available
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