High and low cycle fatigue properties of selective laser melted AISI 316L and AlSi10Mg

In the last years, additive manufacturing has widely adopted to enable lightweight design based on the topological optimization. In fact, this technology allows generation of lattice structures with complex geometries and small thicknesses. In this work, both the low-cycle-fatigue and high-cycle-fatigue behaviors of selective laser melted AISI 316L and AlSi10Mg were investigated. Fatigue samples were designed to characterize small parts and tested in the as-built condition since reticular structures are usually adopted without any finishing operation. Microstructural features were studied by light-optical microscopy and scanning-electron microscopy. Finally, fatigue failures were studied considering the fracture mechanics principles with the Kitagawa-Takahashi diagram. The analysis of fracture surfaces revealed that crack nucleation mainly occurs close to the surface because of both poor surface quality and presence of near-surface defects. As expected, because of the face-centered cubic lattice, the final rupture of all the investigated alloys was characterized by micro-dimples confirming the presence of a ductile behavior

Experimental analysis of seasonal processes in shallow landslide in a snowy region through downscaled and in situ observation

The frequency and intensity of heavy precipitation events increased since the mid-20th century and, considering the climate crisis, it is important also to analyze the effects of processes and events that lead to faster snow mantle melting cycles in mountain areas. Shallow landslides are induced by extreme hydrological events such as the occurrence of short and intense rainfall or by events of medium intensity but prolonged over time. Such slips involve generally reduced portions of land both in area and in thickness, however, they are dangerous due to the absence of warning signals and the lack of knowledge regarding their possible evolution. This work deals with the experimental study of these landslides through the laboratory simulations on a small-scale slope, reproduced at the LIMAG Lab - Laboratory of mountain hydraulics and applied geology of the Lecco Campus and in situ seasonal processes observation at a mountain closed basin nearby Champoluc village in Aosta Valley region. The central objective is to study the evolution of shallow landslides in reduced scale caused by external factor as snowmelt and rainfall and to compare the observations done in laboratory with the ones in situ. In order to investigate the behaviour of shallow landslides in these critical conditions, a series of sensors have been installed on the simulator. This technology includes three modified pressure transmitters for the pore water pressure evaluation which have been accompanied by other support instrumentation consisting of GoPro’s cameras, TDR (Time Domain Reflectometry) and georesistivimeter; all of them provide a cross check of phenomena processes. Throughout the downscaled simulations with snow cover it was possible to observe several processes. The direct interaction between snow and ground does not favor the infiltration of a large amount of water. The protective role of snow lies in keeping the first film of soil at 0 degrees and loading the soil by decreasing its infiltrative capacity; this no longer occurs when the water melted by the snow flows downstream and begins to infiltrate into uncovered and warmer soils. Without thermal or overload barriers, the water pours into the ground. Therefore, a potential susceptible area can be the subject of different filtering and infiltrative contributions from upstream, saturating quickly and collapsing. These laboratory experiments are the starting point for the in-situ analyses and provide a comparison with the observations made by means of ad hoc instrumentation set up at the Champoluc station. Highly detailed information is obtained concerning the density and thickness of the snowpack during seasonal processes. These contribute to defining the hydrogeological processes within the terrain, already studied in the laboratory, and to establishing the water balance

Linking neuroanatomical abnormalities in autism spectrum disorder with gene expression of candidate ASD genes: A meta-analytic and network-oriented approach

BACKGROUND: Autism spectrum disorder (ASD) is a set of developmental conditions with widespread neuroanatomical abnormalities and a strong genetic basis. Although neuroimaging studies have indicated anatomical changes in grey matter (GM) morphometry, their associations with gene expression remain elusive. METHODS: Here, we aim to understand how gene expression correlates with neuroanatomical atypicalities in ASD. To do so, we performed a coordinate-based meta-analysis to determine the common GM variation pattern in the autistic brain. From the Allen Human Brain Atlas, we selected eight genes from the SHANK, NRXN, NLGN family and MECP2, which have been implicated with ASD, particularly in regards to altered synaptic transmission and plasticity. The gene expression maps for each gene were built. We then assessed the correlation between the gene expression maps and the GM alteration maps. Lastly, we projected the obtained clusters of GM alteration-gene correlations on top of the canonical resting state networks, in order to provide a functional characterization of the structural evidence. RESULTS: We found that gene expression of most genes correlated with GM alteration (both increase and decrease) in regions located in the default mode network. Decreased GM was also correlated with gene expression of some ASD genes in areas associated with the dorsal attention and cerebellar network. Lastly, single genes were found to be significantly correlated with increased GM in areas located in the somatomotor, limbic and ganglia/thalamus networks. CONCLUSIONS: This approach allowed us to combine the well beaten path of genetic and brain imaging in a novel way, to specifically investigate the relation between gene expression and brain with structural damage, and individuate genes of potential interest for further investigation in the functional domain

MRI Evidence of Cerebellar and Extraocular Muscle Atrophy Differently Contributing to Eye Movement Abnormalities in SCA2 and SCA28 Diseases.

PURPOSE Spinocerebellar ataxias type 2 and 28 (SCA2, SCA28) are autosomal dominant disorders characterized by progressive cerebellar and oculomotor abnormalities. We aimed to investigate cerebellar, brainstem, and extraocular muscle involvement in the mitochondrial SCA28 disease compared with SCA2. METHODS We obtained orbital and brain 1.5 T-magnetic resonance images (MRI) in eight SCA28 subjects, nine SCA2, and nine age-matched healthy subjects. Automated segmentation of cerebellum and frontal lobe was performed using Freesurfer software. Manual segmentations for midbrain, pons, and extraocular muscles were performed using OsiriX. RESULTS Eye movement abnormalities in SCA2 subjects were characterized by slow horizontal saccades. Subjects with SCA28 variably presented hypometric saccades, saccadic horizontal pursuit, impaired horizontal gaze holding, and superior eyelid ptosis. Quantitative brain MRI demonstrated that cerebellar and pons volumes were significantly reduced in both SCA2 and SCA28 subjects compared with controls (P < 0.03), and in SCA2 subjects compared with SCA28 (P < 0.01). Midbrain and frontal lobe volumes were also significantly reduced in SCA2 compared to controls (P < 0.03), whereas these volumes did not differ between SCA2 and SCA28 and between SCA28 and control subjects. The extraocular muscle areas were 37% to 48% smaller in SCA28 subjects compared with controls (P < 0.002), and 14% to 36% smaller compared with SCA2 subjects (P < 0.03). Extraocular muscle areas did not differ between SCA2 and controls. CONCLUSIONS Our MRI findings support the hypothesis of different cerebellar and extraocular myopathic contributions in the eye movement abnormalities in SCA2 and SCA28 diseases. In SCA28, a myopathic defect selectively involving the extraocular muscles supports a specific impairment of mitochondrial energy metabolism

Clinical utility of Next Generation Sequencing of plasma cell-free DNA for the molecular profiling of patients with NSCLC at diagnosis and disease progression

Background: The present study evaluates the utility of NGS analysis of circulating free DNA (cfDNA), which incorporates small amounts of tumor DNA (ctDNA), at diagnosis or at disease progression (PD) in NSCLC patients. Methods: Comprehensive genomic profiling on cfDNA by NGS were performed in NSCLC patients at diagnosis (if tissue was unavailable/insufficient) or at PD to investigate potential druggable molecular aberrations. Blood samples were collected as routinary diagnostic procedures, DNA was extracted, and the NextSeq 550 Illumina platform was used to run the Roche Avenio ctDNA Expanded Kit for molecular analyses. Gene variants were classified accordingly to the ESCAT score. Results: A total of 106 patients were included in this study; 44&nbsp;% of cases were requested because of tissue unavailability at the diagnosis and 56&nbsp;% were requested at the PD. At least one driver alteration was observed in 62&nbsp;% of cases at diagnosis. Driver druggable variants classified as ESCAT level I were detected in 34&nbsp;% of patients, including ALK-EML4, ROS1-CD74, EGFR, BRAF, KRAS p.G12C, PI3KCA. In the PD group, most patients were EGFR-positive, progressing to a first line-therapy. Sixty-three percent of patients had at least one driver alteration detected in blood and 17&nbsp;% of patients had a known biological mechanism of resistance allowing further therapeutic decisions. Conclusions: The present study confirms the potential of liquid biopsy to detect tumour molecular heterogeneity in NSCLC patients at the diagnosis and at PD, demonstrating that a significant number of druggable mutations and mechanisms of resistance can be detected by NGS analysis on ctDNA

High- and low-cycle-fatigue properties of additively manufactured Inconel 625

In the last years, additive manufacturing has become a widespread technology which enables lightweight-design based on topological optimization. Therefore, generation of lattice structures with complex geometries and small thicknesses is allowed. However, a complete metallurgical and mechanical characterization of these materials is crucial for their effective adoption as alternative to conventionally manufactured alloys. Industrial applications require good corrosion resistance and mechanical strength to provide sufficient reliability and structural integrity. Particularly, fatigue behavior becomes a crucial factor since presence of poor surface finishing can decrease fatigue limits significantly. In this work, both the low-cycle-fatigue and high-cycle-fatigue behaviors of Inconel 625, manufactured by Selective Laser Melting, were investigated. Fatigue samples were designed to characterize small parts and tested in the as-built condition since reticular structures are usually adopted without any finishing operation. Microstructural features were studied by light-optical microscopy and scanning-electron microscopy. Finally, fatigue failures were deeply investigated considering fracture mechanics principles with the Kitagawa-Takahashi diagram