23 research outputs found
An evaluation of the loading direction sensitivity of 3D woven composite with novel web-shaped Z-binder architecture
3D orthogonal (ORT) through-thickness woven composites have excellent out-of-plane properties but exhibit inferior in-plane properties and are loading sensitive. This study proposes a novel web-shaped orientation of z-binder architecture in 3D orthogonal through-thickness model with in-plane uniform elastic moduli to address this drawback. On-axis tensile, Off-axis tensile and In-plane Shear tests were performed on the four macroscale models (ORT _on , WEB _on , ORT _off , WEB _off ). The ORT model, validated by data from the literature, was used as a reference for the proposed WEB model. The overall fibre volume fraction of the WEB _on , and WEB _off models were 7.07% and 8.67% lower than the ORT _on , and ORT _off models. In the on-axis tensile test, the E _x and E _y were lower by 16.89% and 2.66% for WEB _on model, but in the off-axis tensile test, they were higher by 13.75% and 13.77% for WEB _off models. The G _xy was 10.96% lower for WEB _on model. The difference between the E _x and E _y under on-axis loading conditions was 14.81% for ORT model, whereas 0.22% for WEB model. Thus, the proposed WEB shaped z-binder architecture model outperforms the ORT model under the off-axis and is comparable in transverse loading conditions. It also possesses loading direction insensitivity, unlike the ORT model
Not Available
Not AvailableUsing natural processes as inspiration, the present
study demonstrates a positive correlation between zinc
metal tolerance ability of a soil fungus and its potential
for the synthesis of zinc oxide (ZnO) nanoparticles. A total
of 19 fungal cultures were isolated from the rhizospheric
soils of plants naturally growing at a zinc mine area in India
and identified on the genus, respectively the species level.
Aspergillus aeneus isolate NJP12 has been shown to have a
high zinc metal tolerance ability and a potential for extracellular
synthesis of ZnO nanoparticles under ambient conditions.
UV–visible spectroscopy, Fourier transform infrared
spectroscopy, X-ray diffraction analysis, transmission
electron microscopy, and energy dispersive spectroscopy
studies further confirmed the crystallinity, morphology,
and composition of synthesized ZnO nanoparticles. The
results revealed the synthesis of spherical nanoparticles
coated with protein molecules which served as stabilizing
agents. Investigations on the role of fungal extracellular
proteins in the synthesis of nanoparticles indicated that
the process is nonenzymatic but involves amino acids
present in the protein chains.Not Availabl
Not Available
Not AvailableUsing natural processes as inspiration, the present study demonstrates a positive correlation between zinc
metal tolerance ability of a soil fungus and its potential for the synthesis of zinc oxide (ZnO) nanoparticles. A total of 19 fungal cultures were isolated from the rhizospheric soils of plants naturally growing at a zinc mine area in India and identified on the genus, respectively the species level. Aspergillus aeneus isolate NJP12 has been shown to have a high zinc metal tolerance ability and a potential for extracellular synthesis of ZnO nanoparticles under ambient conditions. UV–visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction analysis, transmission electron microscopy, and energy dispersive spectroscopy studies further confirmed the crystallinity, morphology, and composition of synthesized ZnO nanoparticles. The results revealed the synthesis of spherical nanoparticles coated with protein molecules which served as stabilizing
agents. Investigations on the role of fungal extracellular proteins in the synthesis of nanoparticles indicated that
the process is nonenzymatic but involves amino acids present in the protein chains.Not Availabl
Silver Nanoparticles Induce a Triclosan-Like Antibacterial Action Mechanism in Multi-Drug Resistant Klebsiella pneumoniae
International audienceInfections associated with antimicrobial-resistant bacteria now represent a significant threat to human health using conventional therapy, necessitating the development of alternate and more effective antibacterial compounds. Silver nanoparticles (Ag NPs) have been proposed as potential antimicrobial agents to combat infections. A complete understanding of their antimicrobial activity is required before these molecules can be used in therapy. Lysozyme coated Ag NPs were synthesized and characterized by TEM-EDS, XRD, UV-vis, FTIR spectroscopy, zeta potential, and oxidative potential assay. Biochemical assays and deep level transcriptional analysis using RNA sequencing were used to decipher how Ag NPs exert their antibacterial action against multi-drug resistant Klebsiella pneumoniae MGH78578. RNAseq data revealed that Ag NPs induced a triclosan-like bactericidal mechanism responsible for the inhibition of the type II fatty acid biosynthesis. Additionally, released Ag+ generated oxidative stress both extra- and intracellularly in K. pneumoniae. The data showed that triclosan-like activity and oxidative stress cumulatively underpinned the antibacterial activity of Ag NPs. This result was confirmed by the analysis of the bactericidal effect of Ag NPs against the isogenic K. pneumoniae MGH78578 ΔsoxS mutant, which exhibits a compromised oxidative stress response compared to the wild type. Silver nanoparticles induce a triclosan-like antibacterial action mechanism in multi-drug resistant K. pneumoniae. This study extends our understanding of anti-Klebsiella mechanisms associated with exposure to Ag NPs. This allowed us to model how bacteria might develop resistance against silver nanoparticles, should the latter be used in therapy
Removal of Protein Capping Enhances the Antibacterial Efficiency of Biosynthesized Silver Nanoparticles
<div><p>The present study demonstrates an economical and environmental affable approach for the synthesis of “protein-capped” silver nanoparticles in aqueous solvent system. A variety of standard techniques viz. UV-visible spectroscopy, transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS) and X-ray diffraction (XRD) measurements were employed to characterize the shape, size and composition of nanoparticles. The synthesized nanoparticles were found to be homogenous, spherical, mono-dispersed and covered with multi-layered protein shell. In order to prepare bare silver nanoparticles, the protein shell was removed from biogenic nanoparticles as confirmed by UV-visible spectroscopy, FTIR and photoluminescence analysis. Subsequently, the antibacterial efficacy of protein-capped and bare silver nanoparticles was compared by bacterial growth rate and minimum inhibitory concentration assay. The results revealed that bare nanoparticles were more effective as compared to the protein-capped silver nanoparticles with varying antibacterial potential against the tested Gram positive and negative bacterial species. Mechanistic studies based on ROS generation and membrane damage suggested that protein-capped and bare silver nanoparticles demonstrate distinct mode of action. These findings were strengthened by the TEM imaging along with silver ion release measurements using inductively coupled plasma atomic emission spectroscopy (ICP-AES). In conclusion, our results illustrate that presence of protein shell on silver nanoparticles can decrease their bactericidal effects. These findings open new avenues for surface modifications of nanoparticles to modulate and enhance their functional properties.</p></div
(A) XRD spectrum of as-synthesized protein-capped silver nanoparticles with Bragg’s diffraction values shown in parentheses. (B) EDS spectrum showing the elemental composition of silver nanoparticles.
<p>(A) XRD spectrum of as-synthesized protein-capped silver nanoparticles with Bragg’s diffraction values shown in parentheses. (B) EDS spectrum showing the elemental composition of silver nanoparticles.</p
UV visible spectrum of reaction medium as a function of time (0, 12, 24, 48 and 72 h).
<p>Inset shows tubes containing fungal cell-free filtrate (a) without and (b) with silver nitrate solution after 72 h of reaction.</p
(A) A representative transmission electron micrograph showing spherical shaped silver nanoparticles (scale bar equivalent to 50 nm). Inset showing SAED pattern recorded from a single nanoparticle. Particle size distribution histogram of silver nanoparticles as determined using (B) transmission electron microscope and (C) dynamic light scattering measurements.
<p>(A) A representative transmission electron micrograph showing spherical shaped silver nanoparticles (scale bar equivalent to 50 nm). Inset showing SAED pattern recorded from a single nanoparticle. Particle size distribution histogram of silver nanoparticles as determined using (B) transmission electron microscope and (C) dynamic light scattering measurements.</p
Malondialdehyde (MDA) assay demonstrating the difference in membrane damage capability of protein-capped and bare silver nanoparticles.
<p>Vertical bars represent standard errors. Significant differences from control (p ≤ 0.05) are marked with asterisk.</p