Pasteurization profiles of cured meats products with respect to safety

A total of 162 heat penetration sets were collected during commercial pasteurization of 9 types of cured meats. Calculated FP7a-values (with z=10°C) ranged from 45 to 391 min. For L. monocytogenes (D70 =0.27 min) such FP70 represented at least 167 to 1448 decimal reductions (DR) to the initial population of the pathogen. Lack of healing uniformity in lots of the same product and samples in the same chamber was detected. Maximum core temperatures and FP70 values could vary by up to 6.4°C and up to 345 min respectively. Observed FP70 may affect product nutritional value, yield decrease and energy waste. In hundreds of samples of cooked in casing product no Salmonella spp, L.monocytogenes and E.coli were detected immediately after processing. During 2003-5 no Salmonella spp was detected in 404 in retail samples of sliced or pealed meats produced by 8 companies. L.monocytogenes incidence was 7.9%. Incidence per company for sliced and pealed products ranged from 2.5-40% and 0-20% respectively. Incidence decreased with age since production day. Commercial thermal processing of meat products is more than sufficient to assure safety from non-sporoforming pathogens. Cross contaminations lead to isolations of pathogens from cooked and later sliced and repackaged products

The effect of the antioxidant drug U-74389G on endometrial edema during ischemia reperfusion injury in rats

The aim of this experiment was to study the effects of the antioxidant drug U-74389G on rat model, particularly in ischemia reperfusion (IR) protocol. The beneficial or other effects of that molecule were studied pathologically using endometrial edema (EE) lesions. Forty rats were used of mean weight 231.875 gr. EE lesions were evaluated 60 min after reperfusion (groups A and C) and 120 min after reperfusion (groups B and D), with administration of the drug U-74389G in groups C and D. Results were that U-74389G administration non-significantly decreased without lesions the EE scores by 0.41 [-1.00 - 0.17] (p= 0.1607). This finding was in accordance with the results of Wilcoxon signed-rank test (p= 0.5229). Reperfusion time non-significantly increased without lesions the EE scores by 0.21 [-0.38 - 0.81] (p= 0.4701), also in accordance with Wilcoxon signed-rank test (p= 0.1022). However, U-74389G administration and reperfusion time together non-significantly decreased without lesions the EE scores by 0.17 [-0.53 - 0.18] (p= 0.3383). Results of this study indicate that U-74389G administration hardly non-significantly decreases without lesions the EE within short-term time context of 2 hours

Maternal Serum Levels of TNF-Alpha and IL-6 Long after Delivery in Preeclamptic and Normotensive Pregnant Women

Aim. To evaluate maternal TNF-alpha and IL-6 plasma levels in normotensive pregnant women, women with preeclampsia, and to examine the temporal changes in their levels from theantepartum to the postpartum period correlated with the regression of preeclampsia. Method. A prospective study was performed in the 2nd Department of Obstetrics and Gynecology, University of Athens. Blood samples were obtained: (1) antepartum at the time of clinical diagnosis of the syndrome, 2. 12-14 weeks postpartum. Results. No statistically significant differences were found in IL-6 levels, whereas a difference was found in TNF-alpha levels between preeclamptic and controls in antepartum period (0.80 pg/ml versus 0.60 pg/ml, P : .04). Long after delivery, TNF-alpha levels were significantly higher in preeclamptic compared to normotensive controls (0.86 pg/ml versus 0.60 pg/ml, P : .004). No difference was observed in TNF-alpha before and after delivery in both groups. No difference was noticed in IL-6 levels in women of normotensive group long after delivery compared to that before delivery. Long after delivery IL-6 levels were statistically significant higher in preeclamptic women compared to normal controls (3.53 ± 0.52 pg/ml versus 1.69 ± 0.48 pg/ml, P : .02). Conclusion. Preeclamptic women remain under a status of increased inflammatory stress up to 12-14 weeks postpartum despite the fact that all the other signs of preeclampsia are resolved

Prevalence of L.monocytogenes and Listeria spp., in the environment and raw meat products during pig slaughtering, deboning and meat cutting operations.

From 9/2001 –6/2002 we estimated the prevalence of Listeria monocytogenes and Listeria spp., in the environment, and raw pork products of a meat plant. Of 41environmental samples taken before and after initiation of slaughtering, in 2 visits, 10.7% and 7.7% of the samples respectively harbored L. monocytogenes. In each of 2 additional visits we collected 45 samples each time from carcass surfaces. L. monocytogenes was present 2.2% and 2.2% of the samples respectively. Of 109 environmental surface samples from the deboning room before and 104 taken 2-3 h after the beginning of the operation 3.7% and 5.8% harbored L. monocytogenes. Of 132 environmental surfaces samples taken before and 125 after the initiation of work in a special working area handling the cutting and packaging of modified atmosphere (MAP) consumer size meat cuts 3% and 5.6% harbored L. monocytogenes. Of 35 wholesale meat cuts from imported meat collected in the central deboning and cutting room 34.3% harbored L. monocytogenes. Finally of 201 consumer size MAP products prepared from the company`s own pig carcasses, 6% harbored L. monocytogenes. The results indicated the low prevalence of L.monocytogenes on local origin carcasses and MAP cuts prepared from such carcasses under strict sanitary conditions. Cross contaminations of equipment and worker’s hands from imported meats may result in excessive contamination of meat cuts whether the meat is imported or local

Влияние антиоксидантного препарата U-74389G на концентрацию магния при гипоксии–реоксигенации у крыс

Objective: The aim of this experimental study was to examine the effect of the antioxidant drug U-74389G in a rat model of hypoxia reoxygenation using the previously established protocol. Effects of treatments were evaluated by magnesium (Mg2+) levels in blood. Methods: Nonrandomized controlled study was performed. Mg2+ levels were determined in 60 min (groups A and C) and 120 min (groups B and D) after starting the reoxygenation. Groups A and B received no drugs, whereas rats from groups C and D were administered with U-74389G. Results: 40 rats 1618 weeks old of a mean weight of 2312 g were employed in the study. It is demonstrated that U-74389G administration did not alter the Mg2+ levels (decrease in Mg2+ concentration was 0.282.75%; p =0.917). Reoxygenation non-significantly increased the Mg2+ levels by 4.272.66% (p =0.107). Together, the U-74389G administration and reoxygenation non-significantly increased the Mg2+ levels by 0.361.64% (p =0.823). Conclusion: U-74389G administration, alone or in concert with reoxygenation did not significantly affect Mg2+ level in blood after experimental hypoxia in rats.Цель исследования: изучить влияние антиоксидантного лекарственного средства U-74389G на содержание магния (Mg2+) в сыворотке крови на примере крысиной модели гипоксииреоксигенации, используя ранее установленный протокол. Методы: проведено сравнительное нерандомизированное исследование. Эффективность лечения оценивали на основании данных о содержании Mg2+ в сыворотке крови. Концентрацию Mg2+ измеряли после эпизодов реоксигенации длительностью 60 (группы А и С) и 120 мин (группы B и D). Согласно протоколу, группам С и D вводили U-74389G, группам A и B не назначали никаких препаратов. Результаты: в исследовании использовали 40 крыс в возрасте 1618 нед со средней массой тела 231,875 г. Назначение U-74389G существенно не повлияло на концентрацию Mg2+ (отмечено снижение его содержания на 0,282,75%; p =0,917). Увеличение длительности эпизода реоксигенации несущественно повышало уровень Mg2+ (на 4,272,66%; p =0,107). Одновременное введение U-74389G и увеличение длительности эпизода реоксигенации незначительно увеличивали концентрацию Mg2+ (на 0,361,64%; p =0,823). Заключение: применение препарата U-74389G или единовременное его назначение вместе с процессом реоксигенации не оказывают существенного влияния на содержание Mg2+ в крови у крыс после экспериментальной гипоксии

The acute effect of the antioxidant drug “U-74389G” on mean platelet volume levels during hypoxia reoxygenation injury in rats

AbstractBackgroundThis experimental study examined the effect of the antioxidant drug “U-74389G”, on a rat model and particularly in a hypoxia – reoxygenation protocol. The effects of that molecule were studied hematologically using blood mean platelets volume (MPV) levels.Methods40 rats of mean weight 231.875g were used in the study. MPV levels were measured at 60min of reoxygenation (groups A and C) and at 120min of reoxygenation (groups B and D). The drug U-74389G was administered only in groups C and D.ResultsU-74389G administration kept significantly increased the predicted MPV levels by 12.77±3.07% (p=0.0001). Reoxygenation time non-significantly decreased the predicted MPV levels by 2.55±3.71% (p=0.4103). However, U-74389G administration and reoxygenation time together kept significantly increased the predicted MPV levels by 7.09±1.91% (p=0.0005).ConclusionsU-74389G administration whether it interacted or not with reoxygenation time kept significantly increased the predicted MPV levels. This finding has great clinical interest in blood clotting and coagulation pathophysiology

The effect of the antioxidant drug “U-74389G†on testosterone levels during ischemia reperfusion injury in rats

Background: This experimental study examined the effect of the antioxidant drug “U-74389Gâ€, on a rat model and particularly in an adrenal ischemia - reperfusion protocol. The effects of that molecule were studied biochemically using blood mean testosterone (T) levels.Materials and methods: 40 rats of mean weight 231.875 g were used in the study. Testosterone levels were measured at 60 min of reperfusion (groups A and C) and at 120 min of reperfusion (groups B and D), A and B without but C and D with U-74389G administration.Results: U-74389G administration significantly increased the T levels by 52.17%+28.69% (p=0.0451). Reperfusion time significantly decreased the T levels by 85.62%+26.33% (P= 0.0019). However, U-74389G administration and reperfusion time together produced a non-significant combined effect in increasing the T levels by 11.18%+17.97% (p= 0.5245).Conclusions: U-74389G administration interacted or not with reperfusion time increased short – term the testosterone levels.        Â

The effect of erythropoietin on ovarian epithelium edema during ischemia reperfusion injury in rats

Object: This experiment investigated a probable recessing effect of erythropoietin (Epo) in a rat model of ovarian ischemia-reperfusion (IR) injury concerning the mean ovarian epithelium edema (OE) lesions. Methods: 40 rats of mean weight 247.7 g were used totally. The OE lesions scores were evaluated at 60 min (groups A and C) and at 120 min (groups B and D) of reperfusion; after Epo administration in groups C and D. Results: Epo administration non-significantly decreased the OE scores by 0.3 without lesions [-0.8356043 - 0.2356043] (p= 0.2803). Reperfusion time kept non-significantly increased the OE by 0.35 without lesions [-0.2356043 - 0.8356043] (p=0.2557). However, erythropoietin administration and reperfusion time together non-significantly decreased the OE scores by 0.1272727 without lesions [-0.4530022 - 0.1984567] (p=0.4339). Conclusion: Epo administration showed a non-significant short-term recessing trend for OE scores without lesions alteration. Perhaps, a longer study time than 2 hours or a higher Epo dosage may reveal clearer and significant effects. &nbsp

Pasteurization profiles of cured meats products with respect to safety

A total of 162 heat penetration sets were collected during commercial pasteurization of 9 types of cured meats. Calculated FP7a-values (with z=10°C) ranged from 45 to 391 min. For L. monocytogenes (D70 =0.27 min) such FP70 represented at least 167 to 1448 decimal reductions (DR) to the initial population of the pathogen. Lack of healing uniformity in lots of the same product and samples in the same chamber was detected. Maximum core temperatures and FP70 values could vary by up to 6.4°C and up to 345 min respectively. Observed FP70 may affect product nutritional value, yield decrease and energy waste. In hundreds of samples of cooked in casing product no Salmonella spp, L.monocytogenes and E.coli were detected immediately after processing. During 2003-5 no Salmonella spp was detected in 404 in retail samples of sliced or pealed meats produced by 8 companies. L.monocytogenes incidence was 7.9%. Incidence per company for sliced and pealed products ranged from 2.5-40% and 0-20% respectively. Incidence decreased with age since production day. Commercial thermal processing of meat products is more than sufficient to assure safety from non-sporoforming pathogens. Cross contaminations lead to isolations of pathogens from cooked and later sliced and repackaged products.</p

The Effect of the Antioxidant Drug &quot;u-74389G&quot; on Creatinine Levels during Ischemia Reperfusion Injury in Rats

Objective: The aim of this experimental study was to examine the effect of the antioxidant drug &quot;U-74389G&quot; on a rat model using an ischemia reperfusion protocol. The effect of U-74389G was studied biochemically by measuring mean blood creatinine levels. Materials and Methods: Forty rats were used in the study. Creatinine levels were measured at 60 min of reperfusion (groups A and C) or at 120 min of reperfusion (groups B and D), where groups A and B were controls and groups C and D received U-74389G administration. Results: U-74389G administration significantly decreased the predicted creatinine levels by 21.02 ± 5.06% (p = 0.0001). Reperfusion time non-significantly increased the predicted creatinine levels by 4.20 ± 6.12% (p = 0.4103). However, U-74389G administration and reperfusion time together produced a significant combined effect in decreasing the predicted creatinine levels by 11.69 ± 3.16% (p = 0.0005). Conclusion: Independent of reperfusion time, U-74389G administration significantly decreased the creatinine levels in an ischemic rat model. This study demonstrates that short-term U-74389G administration improves renal function by increasing creatinine excretion. © 2015 S. Karger AG, Basel