Background: This experimental study examined the effect of the antioxidant drug “U-74389Gâ€, on a rat model and particularly in an adrenal ischemia - reperfusion protocol. The effects of that molecule were studied biochemically using blood mean testosterone (T) levels.Materials and methods: 40 rats of mean weight 231.875 g were used in the study. Testosterone levels were measured at 60 min of reperfusion (groups A and C) and at 120 min of reperfusion (groups B and D), A and B without but C and D with U-74389G administration.Results: U-74389G administration significantly increased the T levels by 52.17%+28.69% (p=0.0451). Reperfusion time significantly decreased the T levels by 85.62%+26.33% (P= 0.0019). However, U-74389G administration and reperfusion time together produced a non-significant combined effect in increasing the T levels by 11.18%+17.97% (p= 0.5245).Conclusions: U-74389G administration interacted or not with reperfusion time increased short – term the testosterone levels.        Â