L'applicazione della Direttiva ErP presso le piccole e medie imprese: punti di forza e criticità

La Direttiva ErP sulla progettazione ecocompatibile dei prodotti connessi all’energia richiede che le imprese applichino dei criteri di eco-design orientati alla riduzione degli impatti energetico-ambientali lungo tutte le fasi del ciclo di vita dei loro prodotti. In tale contesto la Life Cycle Assessment (LCA) rappresenta uno strumento di fondamentale importanza per individuare le “key issues” energetico-ambientali connesse ai processi produttivi. L’articolo presenta i risultati di una LCA applicata ad una caldaia a biomassa prodotta in Sicilia, evidenziando i punti di forza e le criticità connessi all’attuazione della Direttiva da parte delle piccole e medie imprese

Real-time reverse transcription polymerase chain reaction development for rapid detection of Tomato brown rugose fruit virus and comparison with other techniques

Background: Tomato brown rugose fruit virus (ToBRFV) is a highly infectious tobamovirus that causes severe disease in tomato (Solanum lycopersicum L.) crops. In Italy, the first ToBRFV outbreak occurred in 2018 in several provinces of the Sicily region. ToBRFV outbreak represents a serious threat for tomato crops in Italy and the Mediterranean Basin. Methods: Molecular and biological characterisation of the Sicilian ToBRFV ToB-SIC01/19 isolate was performed, and a sensitive and specific Real-time RT-PCR TaqMan minor groove binder probe method was developed to detect ToBRFV in infected plants and seeds. Moreover, four different sample preparation procedures (immunocapture, total RNA extraction, direct crude extract and leaf-disk crude extract) were evaluated. Results: The Sicilian isolate ToB-SIC01/19 (6,391 nt) showed a strong sequence identity with the isolates TBRFV-P12-3H and TBRFV-P12-3G from Germany, Tom1-Jo from Jordan and TBRFV-IL from Israel. The ToB-SIC01/19 isolate was successfully transmitted by mechanical inoculations in S. lycopersicum L. and Capsicum annuum L., but no transmission occurred in S. melongena L. The developed real-time RT-PCR, based on the use of a primer set designed on conserved sequences in the open reading frames3, enabled a reliable quantitative detection. This method allowed clear discrimination of ToBRFV from other viruses belonging to the genus Tobamovirus, minimising false-negative results. Using immunocapture and total RNA extraction procedures, the real-time RT-PCR and end-point RT-PCR gave the same comparable results. Using direct crude extracts and leaf-disk crude extracts, the end-point RT-PCR was unable to provide a reliable result. This developed highly specific and sensitive real-time RT-PCR assay will be a particularly valuable tool for early ToBRFV diagnosis, optimising procedures in terms of costs and time

Mid-term results of complex distal humeral fractures.

The aim of this study was to assess outcomes following open reduction and internal fixation in complex fractures of the distal humerus. Between 2000 and 2006, 34 patients were operated for complex fractures of the distal humerus. Bone fixation was obtained with a reverse Y-shaped reconstruction plate in 13 cases and with double plating in 21 cases. At final follow-up, all the patients were assessed with the Mayo Elbow Performance Score. Satisfactory results were observed in 71% of the cases despite a high rate of complications. Age over 65 years is correlated with increased risk for an inferior postoperative result. Complex distal humeral fractures are difficult to treat and are associated with a high incidence of complications. It is therefore mandatory to obtain good anatomical reduction and a stable fixation of lateral and medial columns of the distal humerus. The results observed in older patients suggest that an alternative treatment for these patients may be joint replacement

Carga y diversidad microbiana en aire ambiente de sectores de la región del Gran La Plata

Se evaluó la concentración y diversidad de bioaerosoles (hongos y bacterias cultivables) en aire ambiente de la región del Gran La Plata, Provincia de Buenos Aires. Se extrajeron muestras en dos períodos estacionales (invierno y primavera 2005) diferenciando sectores urbanos, industriales, residenciales y rurales en un área aproximada de 25 km2. Los muestreos se realizaron utilizando un método de captación forzada sobre medios selectivos para poblaciones bacterianas oligotróficas y hongos heterótrofos. La caracterización de hongos se realizó mediante observación microscópica hasta nivel de género. Las poblaciones bacterianas se analizaron por extracción de DNA y posterior amplificación del fragmento 16S rDNA mediante la reacción PCR con los iniciadores 341f y 907r. Utilizando la técnica de DGGE se obtuvo el perfil de bandas correspondiente a las poblaciones predominantes de cada sector, luego analizadas mediante taxonomía numérica. Los resultados mostraron un predominio en la concentración de bacterias respecto a hongos, sin evidenciar significativas diferencias entre los sectores seleccionados. Los hongos evidenciaron un predominio estacional (primavera), con prevalencia de Cladosporium sp. En total fueron determinados 18 géneros fúngicos. Se observó elevada diversidad poblacional bacteriana en cada sector estudiado. Se destaca la importancia que reviste la caracterización de bioaerosoles en la zona, tanto por la contribución al conocimiento sobre el diagnóstico de la calidad de aire en la zona, como por las implicancias que reviste sobre la salud ambiental

Road Thermal Collector for Building Heating in South Europe: Numerical Modeling and Design of an Experimental Set-Up

The combination/integration of renewable energy and storage systems appears to have significant potential, achieving high-energy results with lower costs and emissions. One way to cover the thermal needs of a building is through solar energy and its seasonal storage in the ground. The SMARTEP project aims to create an experimental area that provides for the construction of a road solar thermal collector directly connected to a seasonal low-temperature geothermal storage with vertical boreholes. The storage can be connected to a ground-to-water heat pump for building acclimatization. This system will meet the requirements of visual impact and reduction of the occupied area. Nevertheless, several constraints related to the radiative properties of the surfaces and the lack of proper thermal insulation have to be addressed. The project includes the study of several configurations and suitable materials, the set-up of a dynamic simulation model and the construction of a small-scale road thermal collector. These phases allowed for an experimental area to be built. Thanks to careful investigation in the field, it will be possible to identify the characteristics and the best operation strategy to maximize the energy management of the whole system in the Mediterranean area

Are community gardening and horticultural interventions beneficial for psychosocial well-being? A meta-analysis

Recent literature has revealed the positive effect of gardening on human health; however, empirical evidence on the effects of gardening-based programs on psychosocial well-being is scant. This meta-analysis aims to examine the scientific literature on the effect of community gardening or horticultural interventions on a variety of outcomes related to psychosocial well-being, such as social cohesion, networking, social support, and trust. From 383 bibliographic records retrieved (from 1975 to 2019), seven studies with a total of 22 effect sizes were selected on the basis of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Meta-analytic findings on 11 comparisons indicate a positive and moderate effect of horticultural or gardening interventions on psychosocial well-being. Moderation analysis shows a greater effect size in individualistic than collectivistic cultures. A greater effect size was also observed in studies involving community gardening compared to horticultural intervention. Nevertheless, an effect of publication bias and study heterogeneity has been detected. Despite the presence of a large number of qualitative studies on the effect of horticulture/gardening on psychosocial well-being, quantitative studies are lacking. There is a strong need to advance into further high-quality studies on this research topic given that gardening has promising applied implications for human health, the community, and sustainable city management

Public perceptions of forests across Italy: An exploratory national survey

In a context of progressive expansion of the Italian forest area, we present the results of a national survey exploring public perception of forests across different geographical scales in Italy. Perceptions of forests are assessed in rela-tion to popular beliefs on relevant environmental issues such as countering climate change, protecting biodiversity, and promoting social cohesion and environmental education. Participants (N = 1059) living in five different regions of Northern (Trentino-Alto Adige/Südtirol, Piemonte), Central (Lazio, Molise) and Southern Italy (Puglia), were recruited in the survey and completed a paper-and-pencil questionnaire. Survey questions regarded the estimated percentage of forest cover, the perceived importance of different environmental issues and of different material and non-material forest products, as well as partici-pants’ perceptions regarding connectedness to nature. Results revealed a gen-eralized tendency to overestimate the extension of forest surface area in the participants’ region, in Italy, and in the European Union. Results also showed high scores for participants’ perceived importance of environmental issues, such as climate change and biodiversity protection, and in their belief that forests could play a positive role in addressing these issues and providing im-portant outcomes and benefits for the quality of human life, such as health and well-being or social cohesion

Survey of five major grapevine viruses infecting Blatina and Žilavka cultivars in Bosnia and Herzegovina

The sanitary status of grapevines has not yet been considered sufficiently in vineyards throughout Bosnia and Herzegovina (BiH). An extensive survey of five major grapevine viruses in the country was carried out in 2019. A total of 630 samples from the two dominant autochthonous cultivars, namedZ? ilavka and Blatina, were tested by DAS-ELISA for the presence of grapevine leafroll-associated viruses (GLRaV-1 and 3), grapevine fleck virus (GFkV), grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV). Eighty-eight %of the samples were positive for at least one virus, and all five viruses were detected, thought with different incidence, i.e. GLRaV-3 (84%), GFLV (43%), GLRaV-1 (14%), GFkV (10%) and ArMV (0.2%). The majority of infected plants (about 75%) were asymptomatic. Specific virus symptoms were observed in the remaining infected plants, together with the reported GLRaV vectors, Planococcus ficus and Parthenolecanium corni, while nematodes of the Xiphinema genus were not found in the GFLV- or ArMV-infected vineyards. The GLRaV-3 CP phylogenetic analyses showed 75-100% nucleotide identity between the BiH and reference isolates, and the BiH isolates clustered into the major group. The dNS/dS ratio indicated a negative selection of the virus population, and the lack of geographical structuring within the population was observed. In addition, putative GLRaV-3 recombinants with breakpoints in the 5' of the CP gene were detected, while no recombinant strains were identified for the other four viruses. The obtained results indicate a deteriorated sanitary status of the cultivated grapevines, the prevalence and intraspecies genetic diversity of GLRaV-3 throughout the country. The establishment of certified grapevine material and adequate virus vector control is therefore of primary importance to prevent further spread of these viruses. This study presents the results of the first molecular characterisation of grapevine viruses in Bosnia and Herzegovina