Role of Artificial Intelligence in High Throughput Diagnostics for Colorectal Cancer Current Updates

The existence of cancer has been stated as a century’s oldest challenge for the entire human race around theglobe recording a large amount of mortality per year and as per the WHO data nearly 10 million deaths were reported in 2021 worldwide besides others. Colorectal cancer is considered a major threat as this is cancer-related to the colon and rectum with an incidence of 41/1,00,000 recorded annually to overcome this challenge our medical system requires more advanced, accurate and efficient high throughput techniques for the prognosis and effective treatment of this disease. Artificial intelligence’s role in healthcare has been a matter of discussion among experts over the past few years, but more recently the spotlight has focused more specifically on the role that this technology can play in improving patient outcomes and improving the effectiveness of diagnosis and treatment processes. Artificial intelligence refers to a broad category of technologies, including machine learning, natural language processing and deep learning. Exploration of Molecular pathways with characteristics that helps in subtyping of Colorectal Cancer (CRC) leading to specific treatment response or prognosis, for the effective treatment, classification and early detection done using Artificial Intelligence based technologies have shown promising results so far, that it may be utilized to create prediction models in the current environment to distinguish between polyps, metastases, or normal cells in addition to early detection and effective cancer therapy. Nowadays many scientists are putting effort into designing such fabricating models by combining natural language processes and deep learning that can differentiate between non-adenomatous and adenomatous polyps to identify hyper-mutated tumours, genetic mutations and molecular pathways known as IDaRS strategy or iterative draw-and-rank sampling. The review study primarily focuses on the significance of emerging AI-based approaches for the diagnosis, detection, and prognosis of colorectal cancer in light of existing obstacles

White Organic Light Emitting Diodes Via Complementary Color Mixing Approach Through Multilayer Device Architecture

105-110White organic light emitting diodes (WOLEDs) are emerging as potential light emitting technology and have found many applications from back light for liquid crystal displays to next generation displays and solid-state lighting. We report here the fabrication of organic light emitting diodes (OLEDs), which emit white light via multilayer color approach. These WOLEDs incorporated the materials emitting two complementary colors greenish-blue and reddish-orange to make white. The amount of light emitted from each layer was chosen in such a way that the mixture of the two emitted lights gave white light. The amount of light emitted from each layer was controlled by taking optimum thickness of each layer and incorporating a thin hole blocking layer of BCP in between the emissive layers. For greenish-blue light, we used a phosphorescent dopant FIrPic whereas for reddish-orange light we used the phosphorescent dopant Ir(btp)2(acac) and both of these dopants were doped in the host CBP. We studied the effect of operating voltage on the color of the emitted light of these WOLEDs and found that the color of the emitted light was almost independent of operating voltage. The WOLEDs emitted 1600 cd/m2 at 15 V with Commission Internationale de I’Eclairage (CIE) coordinates (0.25, 0.32). We also studied the current density vs voltage (J-V) and voltage vs luminescence (V-L) characteristics of these devices and calculated their efficiency which was found to be 0.35 cd/A. We also prepared some WOLEDs based numeric displays just of the demonstration purpose

Efficient Green Phosphorescent Organic Light Emitting Diode using Iridium Complex

415-421We report here the fabrication and characterization of an efficient green phosphorescent organic light emitting diodes (PhOLED) using phosphorescent iridium complex, tris(2-phenylpyridine) iridium (III) (Ir(ppy)3). Ir(ppy)3 is an organic phosphorescent material, which emits green light via both the singlet as well as triplet exciton relaxation. To avoid triplettriplet annihilation and for efficient light emission, the phosphorescent emitters should be doped in a suitable host material which has band-gap larger than the doped guest material and the band gap of the guest should lie within the band-gap of the host. We optimally doped phosphorescent Ir(ppy)3 into a fluorescent 4,4'-bis(9-carbazolyl) biphenyl (CBP) host and used it as the emitter to prepare PhOLEDs. The PhOLED exhibited green electroluminescence (EL) in the range ~ 480-650 nm with EL peak at 550 nm along with a shoulder at 510 nm. The Commission Internationale de-I’eclairage (CIE) colour coordinate of the device was measured to be (0.25, 0.60). The current density vs voltage vs luminescence (J-V-L) characteristics of the device led us to calculate its current efficiency, which was found to be 28.9 cd/A at 7V. The efficiency of PhOLED decreased with increment in the applied voltage beyond 7 V and has been attributed to the triplet-triplet annihilation due to high injected current densities

Determination of Electron Mobility in Small Molecular1,4-di(bis(8-hydroxyquinoline)aluminum-oxy)benzene by Transient Electroluminescence

Transient electroluminescence is an important tool to determine the charge carrier dynamics in light emitting organic semiconductors. We have used this method to determine the electron mobility in one of the important organic semiconductors 1,4-di(bis(8-hydroxyquinoline)aluminum-oxy)benzene (Alq(1)), used as emissive layer in organic light emitting diodes (OLEDs). For transient electroluminescence studies, we prepared OLEDs using Alq(1) as the emitter. The OLEDs were prepared on indium tin oxide (ITO) coated glass substrates using N, N’-diphenyl -N,N’- bis (3-methylphenyl)-(1,1’-biphenyl)-4,4- diamine (TPD) as hole transport layer (HTL) and lithium fluoride (LiF) as electron injecting buffer layer. The temporal evaluation of the electroluminescence (EL) was studies with respect to a voltage pulse of different amplitudes applied to the device at different temperatures. A delay was observed in the onset of EL from the device with respect to the applied voltage pulse. The EL exhibited a fast initial rise followed by tending to saturate. The EL decayed rapidly as the applied voltage became zero and the decay did not depend upon the amplitude of the applied voltage pulse. The delay time in the onset of EL with respect to the applied voltage pulse is correlated to the electron mobility in Alq(1). The electron mobility in Alq(1) calculated by transient EL method, showed strong dependency on the applied electric field and temperature at low electric fields however at quite high electric fields, the electron mobility in Alq(1) showed poor dependency on the applied electric field and temperature. This behavior of electron mobility in Alq(1) has been explained in terms of shallow charge carrier traps in Alq(1) film. The electron mobility in Alq(1) at 295 K and 2.7´106 V/cm, has been determined to be 5.4´10-6 cm2V-1s-1, which is much higher than that in the well-studied Alq3

A clinical profile of liver function tests in COVID-19 patients at tertiary care centre from north India

Background: The coronavirus is a large group of virus, which spread rapidly as an epidemic in china and was named initially as 2019 novel corona virus and subsequently named as Coronavirus disease 2019 (COVID-19) by World Health Organization (WHO). Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to a dramatic loss of human life globally and manifests a variety of clinical symptoms varying from fever, cough, headache, myalgias, nausea, vomiting to more severe pneumonia, ARDS, septic shock and multiorgan failure. SARS-CoV-2, primarily affect respiratory system but COVID-19 patients also have varying levels of liver injuries or liver dysfunction. This retrospective study was designed to analyze the clinical features, liver function and duration of hospital stay with confirmed cases of covid-19 in a tertiary care centre.Methods: We conducted a cross-sectional study in the Isolation ward, Level -2 Covid Hospital, Government Medical College, Kannauj, Uttar Pradesh (India), from April to June 2021. A detailed history and examination was carried out as per the pre-designed proforma. The liver function test included alanine transaminase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin. Patients were considered with abnormal LFTs when any value of these tests was higher than upper limit of normal.Results: One hundred and ten patients with covid-19 were enrolled during the study period. There were 74 males (67.2%) and 36 (32.7%) females.  The mean age of study population was 49.07±12.05 years. In present study, the mean value of serum bilirubin, SGPT, SGOT and ALP were 0.85±0.47 mg/dl, 74.6±66.9 IU/L, 48.45±36.86 IU/L and 229.25±69.79 IU/L, respectively. In present study, the abnormal liver function was seen in 67.2 % cases with COVID-19 patients. The mean duration of hospital stay among normal LFT and abnormal LFT patients group were 13.33±2.12 and 17.10±2.07 days, respectively.Conclusions: The present study highlighted that abnormal liver function was observed in 67.2% cases with COVID-19 patients. Further research should focus on the cause of liver injury in covid 19 and on treatment and outcome

A Novel Dimer-Tetramer Transition Captured by the Crystal Structure of the HIV-1 Nef

HIV-1 Nef modulates disease progression through interactions with over 30 host proteins. Individual chains fold into membrane-interacting N-terminal and C-terminal core (Nefcore) domains respectively. Nef exists as small oligomers near membranes and associates into higher oligomers such as tetramers or hexadecamers in the cytoplasm. Earlier structures of the Nefcore in apo and complexed forms with the Fyn-kinase SH3 domain revealed dimeric association details and the role of the conserved PXXP recognition motif (residues 72–78) of Nef in SH3-domain interactions. The crystal structure of the tetrameric Nef reported here corresponds to the elusive cytoplasmic stage. Comparative analyses show that subunits of Nefcore dimers (open conformation) swing out with a relative displacement of ∼22 Å and rotation of ∼174° to form the ‘closed’ tetrameric structure. The changes to the association are around Asp125, a conserved residue important for viral replication and the important XR motif (residues 107–108). The tetramer associates through C4 symmetry instead of the 222 symmetry expected when two dimers associate together. This novel dimer-tetramer transition agrees with earlier solution studies including small angle X-ray scattering, analytical ultracentrifugation, dynamic laser light scattering and our glutaraldehyde cross-linking experiments. Comparisons with the Nefcore—Fyn-SH3 domain complexes reveal that the PXXP motif that interacts with the SH3-domain in the dimeric form is sterically occluded in the tetramer. However the 151–180 loop that is distal to the PXXP motif and contains several protein interaction motifs remains accessible. The results suggest how changes to the oligomeric state of Nef can help it distinguish between protein partners

Role of Apoptotic Proteins in REC-2006 Mediated Radiation Protection in Hepatoma Cell Lines

The present study was carried out to evaluate the role of apoptotic proteins in REC-2006-mediated radiation protection in hepatoma cell lines. REC-2006 treatment 2 h before irradiation strongly inhibited the cleavage of ATM and PARP-1 in HepG2 cells. The expression of nuclear apoptosis inducing factor (AIF) was found to be more inhibited (~17%) in HepG2 cells in REC-2006 + radiation-treated group. More inhibition (~33%) of cytochrome c was observed in HepG2 cells upon REC-2006 treatment 2 h prior irradiation. Similarly, significantly more (P<.05) inhibition of Apaf-1, caspase-9 and caspase-3 was observed in REC-2006 + radition-treated group in HepG2 cells. REC-2006 treatment restored the expression of ICAD in HepG2 cells; however, no restoration was observed in Hep3B cells. Lower nuclear to cytoplasmic CAD ratio was observed in HepG2 cells (~0.6) as compared with Hep3B cells (~1.2) in REC-2006 + radiation-treated group. In conclusion, REC-2006 rendered higher protection in HepG2 cells by inhibiting the expression and translocation of AIF, inhibiting the cleavage of ATM and PARP-1, restoring the expression of ICAD, inhibiting the release of cytochrome c and thus modulating the expression of Apaf-1 caspase-9 and activity of caspase-3

Perceived usefulness of social media in financial decision-making: Differences and similarities

Financial decision-making through social media blogs and opinions is an area not much explored by the researchers. This study intends to understand the perceived usefulness of social media in financial decision-making amongst individuals and groups based on demographic similarities and social parameters. This paper aims to understand the perception of various subgroups in society within the large population sample. The paper applies Mann-Whitney and Kruskal-Wallis non-parametric tests to examine the proposed research questions from a dataset of 201 individuals residing in two most populated states in Northern India. The analysis reveals the differences between different groups categorized based on generation, financially dependent, educational background, occupation, and geographical location. In terms of social media's perceived utility in financial decision-making, results suggest that segregated groups based on cohort generation and occupation have significant variations relative to others. Based on the educational context, all other segments, number of financially dependent, geographical location, were found insignificant. The novelty of the paper lies in investigating the perceived usefulness of social media in financial decision-making amongst various homogenous groups based on demographics in a developing country. The study outcomes can be useful for the financial service providers and social media platforms in comprehending consumer behavior to devise an innovative marketing strategy for financial products targeting specific segments through enhanced coordination between them. © This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.IGA/FaME/2019/00

Impedance Spectroscopic Investigation of the Degraded Dye-Sensitized Solar Cell due to Ageing

This paper investigates the effect of ageing on the performance of dye-sensitized solar cells (DSCs). The electrical characterization of fresh and degraded DSCs is done under AM1.5G spectrum and the current density-voltage (J-V) characteristics are analyzed. Short circuit current density (JSC) decreases significantly whereas a noticeable increase in open circuit voltage is observed. These results have been further investigated electroanalytically using electrochemical impedance spectroscopy (EIS). An increase in net resistance results in a lower JSC for the degraded DSC. This decrease in current is mainly due to degradation of TiO2-dye interface, which is observed from light and dark J-V characteristics and is further confirmed by EIS measurements. A reduction in the chemical capacitance of the degraded DSC is observed, which is responsible for the shifting of Fermi level with respect to conduction band edge that further results in an increase of open circuit voltage for the degraded DSC. It is also confirmed from EIS that the degradation leads to a better contact formation between the electrolyte and Pt electrode, which improves the fill factor of the DSC. But the recombination throughout the DSC is found to increase along with degradation. This study suggests that the DSC should be used under low illumination conditions and around room temperature for a longer life