Auxin-binding proteins without KDEL sequence in the moss Funaria hygrometrica

Whereas the important plant growth regulator auxin has multiple effects in flowering plants, it induces a specific cell differentiation step in the filamentous moss protonema. Here, we analyse the presence of classical auxin-binding protein (ABP1) homologues in the moss Funaria hygrometrica. Microsomal membranes isolated from protonemata of F. hygrometrica have specific indole acetic acid-binding sites, estimated to be about 3–5 pmol/mg protein with an apparent dissociation constant (Kd) between 3 and 5 μM. Western analyses with anti-ABP1 antiserum detected the canonical endoplasmic reticulum (ER)-localised 22–24 kDa ABP1 in Zea mays, but not in F. hygrometrica. Instead, polypeptides of 31–33 and 46 kDa were labelled in the moss as well as in maize. In F. hygrometrica these proteins were found exclusively in microsomal membrane fractions and were confirmed as ABPs by photo-affinity labelling with 5-azido-[7-3H]-indole-3-acetic acid. Unlike the classical corn ABP1, these moss ABPs did not contain the KDEL ER retention sequence. Consistently, the fully sequenced genome of the moss Physcomitrella patens, a close relative of F. hygrometrica, encodes an ABP1-homologue without KDEL sequence. Our study suggests the presence of putative ABPs in F. hygrometrica that share immunological epitopes with ABP1 and bind auxin but are different from the classical corn ABP1

Bioremediation of Heavy Metals: A Substantive Potential for Clean Earth

Modernindustrialand agriculture developments, anthropogenic activities effectedterrestrial and aquatic pollution of heavy metalsposing serious threatsto biotic (human/animal/plant) and environmental health, as well as, welfareofany nation.Phytoremediation of metal pollutedsiteshas attracted worldwide significancedue to its being less expensiveand environment-friendlyproperties. Robust information is availableon heavy metal remediation, phytoremediation and severalplants are recordedworldwidewhich hyper accruewide category ofheavy metals. This communication emphasizesvariousremediation methods,especially phytoremediationfor heavy metalsalong withits advantage compared to othertechniques. Phytoremediation techniqueis specifically applicable for reclamationofsites with low to moderatepollutionleveldue to its being energyefficientand aesthetically pleasant, alsoit can be co-practised with variousconventionalremedial processesas anultimateprocedurefor remediationprocess

Salt and drought stress responses in cultivated beets (Beta vulgaris L.) and wild beet (Beta maritima L.)

Cultivated beets, including leaf beets, garden beets, fodder beets, and sugar beets, which belong to the species Beta vulgaris L., are economically important edible crops that have been originated from a halophytic wild ancestor, Beta maritima L. (sea beet or wild beet). Salt and drought are major abiotic stresses, which limit crop growth and production and have been most studied in beets compared to other environmental stresses. Characteristically, beets are salt- and drought-tolerant crops; however, prolonged and persistent exposure to salt and drought stress results in a significant drop in beet productivity and yield. Hence, to harness the best benefits of beet cultivation, knowledge of stress-coping strategies, and stress-tolerant beet varieties, are prerequisites. In the current review, we have summarized morpho-physiological, biochemical, and molecular responses of sugar beet, fodder beet, red beet, chard (B. vulgaris L.), and their ancestor, wild beet (B. maritima L.) under salt and drought stresses. We have also described the beet genes and noncoding RNAs previously reported for their roles in salt and drought response/tolerance. The plant biologists and breeders can potentiate the utilization of these resources as prospective targets for developing crops with abiotic stress tolerance

Integrated Expression Analysis of Small RNA, Degradome and Microarray Reveals Complex Regulatory Action of miRNA during Prolonged Shade in Swarnaprabha Rice

Prolonged shade during the reproductive stage can result in significant yield losses in rice. For this study, we elucidated the role of microRNAs in prolonged-shade tolerance (~20 days of shade) in a shade-tolerant rice variety, Swarnaprabha (SP), in its reproductive stage using small RNA and degradome sequencing with expression analysis using microarray and qRT-PCR. This study demonstrates that miRNA (miR) regulation for shade-tolerance predominately comprises the deactivation of the miR itself, leading to the upregulation of their targets. Up- and downregulated differentially expressed miRs (DEms) presented drastic differences in the category of targets based on the function and pathway in which they are involved. Moreover, neutrally regulated and uniquely expressed miRs also contributed to the shade-tolerance response by altering the differential expression of their targets, probably due to their differential binding affinities. The upregulated DEms mostly targeted the cell wall, membrane, cytoskeleton, and cellulose synthesis-related transcripts, and the downregulated DEms targeted the transcripts of photosynthesis, carbon and sugar metabolism, energy metabolism, and amino acid and protein metabolism. We identified 16 miRNAs with 21 target pairs, whose actions may significantly contribute to the shade-tolerance phenotype and sustainable yield of SP. The most notable among these were found to be miR5493-OsSLAC and miR5144-OsLOG1 for enhanced panicle size, miR5493-OsBRITTLE1-1 for grain formation, miR6245-OsCsIF9 for decreased stem mechanical strength, miR5487-OsGns9 and miR168b-OsCP1 for better pollen development, and miR172b-OsbHLH153 for hyponasty under shade

Cold Tolerance Mechanisms in Mungbean (<i>Vigna radiata</i> L.) Genotypes during Germination

Mungbean or greengram (Vigna radiata) is an important legume crop well known for its high protein with nitrogen-fixing abilities. However, the severe yield loss in mungbean occurs due to susceptibility to low temperatures at all stages of plant growth including germination and is a serious concern for its cultivation and productivity. To select cold-tolerant genotypes, a germination-based screening at 10 °C was performed in a total of 204 germplasms. The study showed that cold stress of the initial 8-days during seedling establishment imposed a negative impact throughout the life of mungbean genotypes, which were reflected in the vegetative and reproductive phase (plant height, days to 50% flowering and pods/plant, seeds/pod, yield/plant, and 100-seed weight). The biplot analysis showed that parameters such as germination rate index, Timson’s index, mean germination time, and coefficient of the velocity of germination are the key influential germination parameters for identifying cold tolerance in the seedling stage. Identified cold-tolerant genotype (PAU911) retained higher rootlet number, leaf area, and increased chlorophyll, carotenoid, and malondialdehyde (MDA) content at 10 °C. Based on the confocal microscopic study, it is noticed that the stomatal density, open pore percentage, and trichome density were significant differences in seedlings exposed to cold stress as compared to non-stress. On the basis of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) analysis, it is observed that a new protein identified as TETRATRICOPEPTIDE-REPEAT THIOREDOXIN-LIKE1 (TTL1) (UNIPROT Identifier: LOC106762419) which highly correlated with the cold stress response of in the cold-tolerant genotype. Our study identifies a noble member, TTL1, whose expression has a positive role in cold tolerance response at the protein level in V. radiata. This study will help breeding programs with regard to the sustainable growth of mungbean

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Not AvailableSoils deficient in P are widespread in major rice ecosystems. In view of declining reserves of rock phosphate and rising costs of P-fertilizers, breeding rice varieties tolerant to low P becomes important for future food security. Four different methods 1. Hydroponics without sand (H), 2. Hydroponics with sand (HS), 3. Large pots with soil (PS) and 4. Glasses with soil (GS) were evaluated using rice aus variety Nagina 22 (N22) and its known gain/loss of function (gof/lof) mutants to screen for low P-tolerance in field. In –P shoot dry weight was significantly more in gof mutant NH787 than in N22 in HS, PS and GS but not in H with fold increase of 1.8 in HS, 5.2 in GS and 9.4 in PS. In HS, in -P, out of 6 traits only shoot dry weight was significantly higher in gof and lower in lof mutants. However, in GS both root and shoot dry weight could confirm gof and lof mutants. It took 40d in GS and 70d in PS to differentiate between growth in –P/ low P and +P and also between gof and lof mutants. Thus shoot dry weight at 30d in HS and both root and shoot dry weight at 40d in GS are best to differentiate between genotypes grown in –P/lowP and +P and also between gof and lof mutants for low P tolerance. The HS method can be carried out in ambient conditions and needs 70% lesser medium compared to H. If germplasm is to be screened for low P tolerance on a large scale, and there is no access to low P soil, then screening using HS is best.Not Availabl

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Not AvailableHeat is one of the major factors that considerably limit rice production. Nagina 22 (N22) is a deep-rooted, drought and heat tolerant aus rice cultivar. This study reports the characterization of a previously isolated dark green leaf mutant N22-H-dgl219 (NH219) which showed reduced accumulation of reactive oxygen species in leaf under 40°C heat conditions. The mutant was characterized for several traits in field under ambient (38°C) and heat stress (44°C) conditions by raising temperature artificially from flowering stage till maturity by covering plants with polythene sheets during dry season 2011. Yield traits were mapped in 70 F2 segregants of IR64 × NH219 and 36 F2 segregants of its reciprocal cross.Not Availabl