Ocho respuestas sobre la Leucosis Enzoótica Bovina

En el artículo se responden las siguientes preguntas: - ¿Qué es la Leucosis Enzoótica Bovina? - ¿Cuáles son las vías de trasmisión de la enfermedad? - ¿Se curan los bovinos con LEB, cuál es la manifestación clínica de la enfermedad? - ¿Es transmisible el VLB al ser humano? - ¿Se puede consumir la leche y elaborar productos lácteos de animales positivos a LEB? - Epidemiología y control: ¿Qué hay que hacer con el rodeo infectado? - ¿Cuánto es la pérdida productiva de un rodeo lechero con LEB? - ¿Existen vacunas contra el VLB?Universidad Nacional de La Plata (UNLP) - Facultad de Ciencias Agrarias y Forestales (FCAF

Ocho respuestas sobre la Leucosis Enzoótica Bovina

En el artículo se responden las siguientes preguntas: - ¿Qué es la Leucosis Enzoótica Bovina? - ¿Cuáles son las vías de trasmisión de la enfermedad? - ¿Se curan los bovinos con LEB, cuál es la manifestación clínica de la enfermedad? - ¿Es transmisible el VLB al ser humano? - ¿Se puede consumir la leche y elaborar productos lácteos de animales positivos a LEB? - Epidemiología y control: ¿Qué hay que hacer con el rodeo infectado? - ¿Cuánto es la pérdida productiva de un rodeo lechero con LEB? - ¿Existen vacunas contra el VLB?Universidad Nacional de La Plata (UNLP) - Facultad de Ciencias Agrarias y Forestales (FCAF

Enzyme-Linked Immunosorbent Assays Using the Recombinant gp51 and p24 of Bovine Leukemia Virus for Immunodetection of the Disease

Enzyme-linked immunosorbent assay (ELISA) is often used to test bovineleukemia virus (BLV) infection. However, commercially available kits test inSouth America detect only antibodies against the gp51 protein. With the aimto improve the sensitivity of the test, we developed here a two-step indirectdual ELISA test that included both proteins p24 and gp51, expressed andproduced in E. coli and baculovirus expression system respectively. Two hundredten BLV sera, stated as double positive or double negative by the combinationof commercial agar gel immunodiffusion (AGID) assay and a gp51-ELISA test, were tested with our in house dual rp24/rgp51 ELISA. Firstly, we checked the purified, optimized and standardized proteins as antigen by the checkerboard technique, and set up our in house ELISA test. The concordancecorrelation coefficient (CCC) and coefficient of variation (CV) intraplate repeatability levels were within the values established by the international standards.The statistical analysis demonstrated the value of sera correctly rankedhighest (93.48%), and for 0.3 cutoff, the sensitivity was 95.65% and the specificity 91.30%. In conclusion, the rp24/rgp51 ELISA developed and standardized here demonstrated to have good analytical characteristics to be considered for screening of BLV.Fil: Larsen, Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Corva, Santiago. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Panei, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; ArgentinaFil: Geisler, Christoph. University Of Wyoming; Estados UnidosFil: Mortola, Eduardo. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentin

Morphological and Molecular Characterization of a New Trichuris Species (Nematoda- Trichuridae), and Phylogenetic Relationships of Trichuris Species of Cricetid Rodents from Argentina

Populations of Trichuris spp. isolated from six species of sigmodontine rodents from Argentina were analyzed based on morphological characteristics and ITS2 (rDNA) region sequences. Molecular data provided an opportunity to discuss the phylogenetic relationships among the Trichuris spp. from Noth and South America (mainly from Argentina). Trichuris specimens were identified morphologically as Trichuris pardinasi, T. navonae, Trichuris sp. and Trichuris new species, described in this paper. Sequences analyzed by Maximum Parsimony, Maximum Likelihood and Bayesian inference methods showed four main clades corresponding with the four different species regardless of geographical origin and host species. These four species from sigmodontine rodents clustered together and separated from Trichuris species isolated from murine and arvicoline rodents (outgroup). Different genetic lineages observed among Trichuris species from sigmodontine rodents which supported the proposal of a new species. Moreover, host distribution showed correspondence with the different tribes within the subfamily Sigmodontinae.Fil: Robles, Maria del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaFil: Cutilla, Cristina. Universidad de Sevilla; EspañaFil: Panei, Carlos Javier. Universidad Nacional de la Plata. Facultad de Ciencias Veterinarias. Departamento de Microbiología. Cátedra de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Callejón, Rocío. Universidad de Sevilla; Españ

Morphological and Molecular Characterization of a New Trichuris Species (Nematoda- Trichuridae), and Phylogenetic Relationships of Trichuris Species of Cricetid Rodents from Argentina

Populations of Trichuris spp. isolated from six species of sigmodontine rodents from Argentina were analyzed based on morphological characteristics and ITS2 (rDNA) region sequences. Molecular data provided an opportunity to discuss the phylogenetic relationships among the Trichuris spp. from Noth and South America (mainly from Argentina). Trichuris specimens were identified morphologically as Trichuris pardinasi, T. navonae, Trichuris sp. and Trichuris new species, described in this paper. Sequences analyzed by Maximum Parsimony, Maximum Likelihood and Bayesian inference methods showed four main clades corresponding with the four different species regardless of geographical origin and host species. These four species from sigmodontine rodents clustered together and separated from Trichuris species isolated from murine and arvicoline rodents (outgroup). Different genetic lineages observed among Trichuris species from sigmodontine rodents which supported the proposal of a new species. Moreover, host distribution showed correspondence with the different tribes within the subfamily Sigmodontinae.Centro de Estudios Parasitológicos y de VectoresFacultad de Ciencias Veterinaria

Enzyme-Linked Immunosorbent Assays Using the Recombinant gp51 and p24 of Bovine Leukemia Virus for Immunodetection of the Disease

Enzyme-linked immunosorbent assay (ELISA) is often used to test bovine leukemia virus (BLV) infection. However, commercially available kits test in South America detect only antibodies against the gp51 protein. With the aim to improve the sensitivity of the test, we developed here a two-step indirect dual ELISA test that included both proteins p24 and gp51, expressed and produced in E. coli and baculovirus expression system respectively. Two hundred ten BLV sera, stated as double positive or double negative by the combination of commercial agar gel immunodiffusion (AGID) assay and a gp51- ELISA test, were tested with our in house dual rp24/rgp51 ELISA. Firstly, we checked the purified, optimized and standardized proteins as antigen by the checkerboard technique, and set up our in house ELISA test. The concordance correlation coefficient (CCC) and coefficient of variation (CV) intraplate repeatability levels were within the values established by the international standards. The statistical analysis demonstrated the value of sera correctly ranked highest (93.48%), and for 0.3 cutoff, the sensitivity was 95.65% and the specificity 91.30%. In conclusion, the rp24/rgp51 ELISA developed and standardized here demonstrated to have good analytical characteristics to be considered for screening of BLV.Facultad de Ciencias Veterinaria

Examen para WSSV en crustáceos de áreas marinas de Buenos Aires, Argentina

In total 374 specimens corresponding to four species of shrimp: Artemesia longinaris, Pleoticus muelleri, Peisospetrunkevitchii, Palaemon macrodactylus, and two species of crabs: Neohelice granulata and Cyrtograpsus angulatus were studiedin the search of white spot syndrome virus (WSSV). The crustaceans were collected in the estuary of Bahia Blanca, the port of Mardel Plata and the Samborombón Bay, Argentina, between 2010 and 2014. A polymerase chain reaction (PCR) analysis in all of themhas not detected any virus presence. These new results suggest that the discovery of infected shrimp in 2008-09 in the BahiaBlanca estuary may have been a one-time local event, promoted by special conditions of seawater temperature inside the estuary,coincident with the presence of a virus carrier or transporter. To determine if the virus was finally installed in the estuary of BahiaBlanca more screening are needed coming from a larger number of shrimp samples. These studies will be necessary mainly in A.longinaris (Penaeidae) since this family of shrimps is most susceptible to be affected by WSSV.Centro de Estudios Parasitológicos y de VectoresFacultad de Ciencias Veterinaria

First isolation and nucleotide comparison of the gag gene of the caprine arthritis encephalitis virus circulating in naturally infected goats from Argentina

Caprine arthritis encephalitis virus (CAEV) has been reported in different countries worldwide, based on serological and molecular detection. In Argentina, the prevalence of CAEV infections is increasing, with goats showing symptoms associated mostly with cachexia and arthritis. Although in Argentina the virus has been detected by serology, it has never been isolated or characterized. Thus, the objectives of this work were to isolate and analyze the nucleotide sequences of the gag gene of Argentine CAEV strains and compare them with those of other SRLVs previously reported. Nucleotide sequence comparison showed homology with CAEV-Co, the CAEV prototype. Phylogenetic analyses showed that the Argentine strains clustered with genotype B, subtype B1. Because the molecular characterization of the gag region is suitable for phylogenetic studies and may be applied to monitor the control of SRLV, molecularly characterizing the Argentine CAEV strains may help develop a proper plan of eradication of CAEV infections.Facultad de Ciencias Veterinaria

Evaluation of apoptosis markers in different cell lines infected with equine arteritis virus

Equine arteritis virus (EAV) induces apoptosis in infected cells. Cell death caused by EAV has been studied mainly using three cell lines, BHK-21, RK-13 and Vero cells. The mechanism of apoptosis varies among cell lines and results cannot be correlated owing to differences in EAV strains used. We evaluated different markers for apoptosis in BHK-21, RK-13 and Vero cell lines using the Bucyrus EAV reference strain. Acridine orange/ethidium bromide staining revealed morphological 10 changes in infected cells, while flow cytometry indicated the extent of apoptosis. We also observed DNA fragmentation, but the DNA ladder was detected at different times post-infection depending on the cell line, i.e., 48, 72 and 96 h post-infection in RK-13, Vero and BHK-21 cells, respectively. Measurement of viral titers obtained with each cell line indicated that apoptosis causes interference with viral replication and therefore decreased viral titers. As an unequivocal 15 marker of apoptosis, we measured the expression of caspase-3 and caspases-8 and −9 as extrinsic and intrinsic markers of apoptosis pathways, respectively. Caspase-8 in BHK-21 cells was the only protease that was not detected at any of the times assayed. We found that Bucyrus EAV strain exhibited a distinctive apoptosis pathway depending on the cell line.Facultad de Ciencias Veterinaria

Evaluation of apoptosis markers in different cell lines infected with equine arteritis virus

Equine arteritis virus (EAV) induces apoptosis in infected cells. Cell death caused by EAV has been studied mainly using three cell lines, BHK-21, RK-13 and Vero cells. The mechanism of apoptosis varies among cell lines and results cannot be correlated owing to differences in EAV strains used. We evaluated different markers for apoptosis in BHK-21, RK-13 and Vero cell lines using the Bucyrus EAV reference strain. Acridine orange/ethidium bromide staining revealed morphological 10 changes in infected cells, while flow cytometry indicated the extent of apoptosis. We also observed DNA fragmentation, but the DNA ladder was detected at different times post-infection depending on the cell line, i.e., 48, 72 and 96 h post-infection in RK-13, Vero and BHK-21 cells, respectively. Measurement of viral titers obtained with each cell line indicated that apoptosis causes interference with viral replication and therefore decreased viral titers. As an unequivocal 15 marker of apoptosis, we measured the expression of caspase-3 and caspases-8 and −9 as extrinsic and intrinsic markers of apoptosis pathways, respectively. Caspase-8 in BHK-21 cells was the only protease that was not detected at any of the times assayed. We found that Bucyrus EAV strain exhibited a distinctive apoptosis pathway depending on the cell line.Facultad de Ciencias Veterinaria