2,440 research outputs found

    Computational approaches to shed light on molecular mechanisms in biological processes

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    Computational approaches based on Molecular Dynamics simulations, Quantum Mechanical methods and 3D Quantitative Structure-Activity Relationships were employed by computational chemistry groups at the University of Milano-Bicocca to study biological processes at the molecular level. The paper reports the methodologies adopted and the results obtained on Aryl hydrocarbon Receptor and homologous PAS proteins mechanisms, the properties of prion protein peptides, the reaction pathway of hydrogenase and peroxidase enzymes and the defibrillogenic activity of tetracyclines. © Springer-Verlag 2007

    Fatigue behavior and cyclic damage of peek short fiber reinforced composites

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    Fatigue strength and failure mechanisms of short fiber reinforced (SFR) PEEK have been investigated in the past by several research groups. However some relevant aspects of the fatigue behavior of these materials, like cyclic creep and fatigue damage accumulation and modeling, have not been studied yet, in particular in presence of both fillers and short fibers as reinforcement. In the present research these aspects were considered by carrying out uni-axial fatigue tests in load control (cycle ratio R = 0) on neat PEEK and PEEK based composites reinforced either with short carbon fibers only or with addition of fillers (graphite and PTFE). For each material stress-life curves were obtained and compared. Fatigue fracture surfaces were analyzed to identify failure mechanisms in presence of different reinforcement types. The evolution of cyclic creep strain was also monitored as a function of the number of cycles, thus allowing investigation on the correlation between cyclic creep parameters and fatigue life. The evolution of cyclic damage with loading cycles was then compared by defining a damage parameter related to the specimen stiffness reduction observed during the tests. Progressive cyclic damage evolution of short fiber reinforced PEEK composites presented significantly different patterns depending on applied stress level and on the presence of different reinforcement typologies. In order to reproduce the different fatigue damage kinetics and stages of progressive damage accumulation observed experimentally, a cyclic damage model was finally developed and implemented into a finite element code by which a satisfactory agreement between numerical prediction and experimental data at different stress levels for each examined material

    Structural and functional diversity of ferredoxin-NADP+ reductases

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    Although all ferredoxin-NADP+ reductases (FNRs) catalyze the same reaction, i.e. the transfer of reducing equivalents between NADP(H) and ferredoxin, they belong to two unrelated families of proteins: the plant-type and the glutathione reductase-type of FNRs. Aim of this review is to provide a general classification scheme for these enzymes, to be used as a framework for the comparison of their properties. Furthermore, we report on some recent findings, which significantly increased the understanding of the structure–function relationships of FNRs, i.e. the ability of adrenodoxin reductase and its homologs to catalyze the oxidation of NADP+ to its 4-oxo derivative, and the properties of plant-type FNRs from non-photosynthetic organisms. Plant-type FNRs from bacteria and Apicomplexan parasites provide examples of novel ways of FAD- and NADP(H)-binding. The recent characterization of an FNR from Plasmodium falciparum brings these enzymes into the field of drug design

    BCR-ABL residues interacting with ponatinib are critical to preserve the tumorigenic potential of the oncoprotein

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    Patients with chronic myeloid leukemia in whom tyrosine kinase inhibitors (TKIs) fail often present mutations in the BCR-ABL catalytic domain. We noticed a lack of substitutions involving 4 amino acids (E286, M318, I360, and D381) that form hydrogen bonds with ponatinib. We therefore introduced mutations in each of these residues, either preserving or altering their physicochemical properties. We found that E286, M318, I360, and D381 are dispensable for ABL and BCR-ABL protein stability but are critical for preserving catalytic activity. Indeed, only a "conservative" I360T substitution retained kinase proficiency and transforming potential. Molecular dynamics simulations of BCR-ABLI360T revealed differences in both helix αC dynamics and protein-correlated motions, consistent with a modified ATP-binding pocket. Nevertheless, this mutant remained sensitive to ponatinib, imatinib, and dasatinib. These results suggest that changes in the 4 BCR-ABL residues described here would be selected against by a lack of kinase activity or by maintained responsiveness to TKIs. Notably, amino acids equivalent to those identified in BCR-ABL are conserved in 51% of human tyrosine kinases. Hence, these residues may represent an appealing target for the design of pharmacological compounds that would inhibit additional oncogenic tyrosine kinases while avoiding the emergence of resistance due to point mutations.This work was supported by an investigator grant to P.V. from Associazione Italiana per la Ricerca sul Cancro (AIRC) and by funding from the Biotechnology and Biological Sciences Research Council (BB/I023291/1 and BB/H018409/1 to AP and FF). P.B. is the recipient of an AIRC - Marie Curie fellowship

    Stress-Free Two-Way Shape Memory Effect of Poly(ethylene glycol)/ Poly(epsilon-caprolactone) Semicrystalline Networks

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    In this work, poly(ethylene glycol) (PEG)/poly(epsilon- caprolactone) (PCL) semicrystalline networks were prepared by photo-cross-linking of methacrylated macromonomers with different molecular weights and in different proportions to obtain amphiphilic materials capable of displaying properly designed shape memory effects. Networks based on PCL 10 kDa and PEG 3 kDa showed suitable thermal and mechanical properties with well-separated crystallization and melting regions to achieve a self-standing two-way shape memory effect. Particularly, after the application of a specific thermomechanical history, these materials are capable of cyclically changing their shape between two configurations upon cooling-heating cycles in the absence of any external load applied. The effect of the composition of the networks and of the employed thermomechanical parameters, such as the applied strain and the actuation temperature, was investigated to shed light on the shape memory mechanism for this class of materials, which are considered promising for applications in the biomedical field and as reversible actuators for soft robotics

    Reversible Stress-Driven and Stress-Free Two-Way Shape Memory Effect in a Sol-Gel Crosslinked Polycaprolactone

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    The two-way shape memory effect is the ability of a material to change its shape between two configurations upon application and removal of a stimulus, and, among shape memory polymers, it is featured only by few systems, such as semicrystalline networks. When studied under tensile conditions, it consists of elongation-contraction cycles along cooling and heating across the crystallization and melting region, typically under the application of a constant load. However, recent studies on crosslinked semicrystalline co-polymers demonstrate that also a completely stress-free, or self-sustained, two-way effect may be achieved through specific thermomechanical cycles. This effect is currently regarded with interest for the development of intrinsically reversible sensors and actuators, and it may also be displayed by simpler materials, as homopolymer-based semicrystalline networks. Only seldom articles investigate this possibility, therefore in this work the two-way shape memory behavior is studied on a poly(e-caprolactone) system, crosslinked by means of a sol-gel approach. The effect is studied both under stress-driven and stress-free condition, by applying properly set-up thermo-mechanical histories. The results allow to describe the effect as a function of temperature, to reveal the dependence on specific testing parameters and to compare the extent of the reversible strain variation under these two conditions

    Is renalase a novel player in catecholaminergic signaling? The mystery of the catalytic activity of an intriguing new flavoenzyme

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    Renalase is a flavoprotein recently discovered in humans, preferentially expressed in the proximal tubules of the kidney and secreted in blood and urine. It is highly conserved in vertebrates, with homologs identified in eukaryotic and prokaryotic organisms. Several genetic, epidemiological, clinical and experimental studies show that renalase plays a role in the modulation of the functions of the cardiovascular system, being particularly active in decreasing the catecholaminergic tone, in lowering blood pressure and in exerting a protective action against myocardial ischemic damage. Deficient renalase synthesis might be the cause of the high occurrence of hypertension and adverse cardiac events in kidney disease patients. Very recently, recombinant human renalase has been structurally and functionally characterized in vitro. Results show that it belongs to the p-hydroxybenzoate hydroxylase structural family of flavoenzymes, contains non-covalently bound FAD with redox features suggestive of a dehydrogenase activity, and is not a catecholamine-degrading enzyme, either through oxidase or NAD(P)H-dependent monooxygenase reactions. The biochemical data now available will hopefully provide the basis for a systematic and rational quest toward the identification of the reaction catalyzed by renalase and of the molecular mechanism of its physiological action, which in turn are expected to favor the development of novel therapeutic tools for the treatment of kidney and cardiovascular diseases

    The Inorganic Side of NGF: Copper(II) And Zinc(II) Affect the NGF Mimicking Signalling of the N-Terminus Peptides Encompassing the Recognition Domain of TrkA Receptor

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    The nerve growth factor (NGF) N-terminus peptide, NGF(1-14), and its acetylated form, Ac-NGF(1-14), were investigated to scrutinise the ability of this neurotrophin domain to mimic the whole protein. Theoretical calculations demonstrated that non-covalent forces assist the molecular recognition of TrkA receptor for both peptides. Combined parallel tempering/docking simulations discriminated the effect of the N-terminal acetylation on the recognition of NGF(1-14) towards the domain 5 of TrkA (TrkA-D5). Experimental findings demonstrated that both NGF(1-14) and Ac-NGF(1-14) activate TrkA signaling pathways essential for neuronal survival. The NGF-induced TrkA internalization was slightly inhibited in the presence of Cu2+ and Zn2+ ions, whereas the metal ions elicited the NGF(1-14)-induced internalization of TrkA and no significant differences were found in the weak Ac-NGF(1-14)-induced receptor internalization. The crucial role of the metals was confirmed by experiments with the metal-chelator bathocuproine disulfonic acid, which discriminated different levels of inhibitory effects in the signalling cascade, due to different metal affinity of NGF, the free amino and the acetylated peptides. The NGF signaling cascade, activated by NGF (1−14) and Ac-NGF(1-14), induced CREB phosphorylation, but the copper addition further stimulated the Akt, ERK and CREB phosphorylation only for NGF and NGF(1-14). A dynamic and quick influx of both peptides into PC12 cells was tracked by live cell imaging with confocal microscopy. A significant role of copper ions was found in the modulation of peptide sub-cellular localization, especially at the nuclear level. Furthermore, a strong copper ionophoric ability of NGF(1-14) was measured. The Ac-NGF(1-14) peptide, which binds copper ions with a lower stability constant than NGF(1-14), exhibited a lower nuclear localization with respect to the total cellular uptake. These findings were correlated to the metal-induced increase of CREB and BDNF expression upon NGF(1-14) stimulation. In summary, we here validate NGF(1-14) and Ac-NGF(1-14) as first examples of monomer and linear peptides able to activate the NGF-TrkA signaling cascade. Metal ions modulate the activity of both NGF protein and the NGF-mimicking peptides. Such findings demonstrate that NGF(1-14) sequence can reproduce the signal transduction of whole protein, therefore represent a very promising drug candidate for further preclinical studies

    RNA-seq dataset of subcutaneous adipose tissue: Transcriptional differences between obesity and healthy women

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    In this data article, we present the dataset from the RNA-Seq analysis of subcutaneous adipose tissue collected from 5 healthy normal weight women (NW, age 37 +/- 6.7 years, BMI 24.3 +/- 0.9 kg/m(2)) and 5 obese women (OBF, age 41 +/- 12.5 years, BMI 38.2 +/- 4.6 kg/m(2)). Raw data obtained from Illumina NextSeq 500 sequencer were processed through BlueBee (R) Genomics Platform while differential expression analysis was performed with the DESeq2 R package and deposited in the GEO public repository with GSE166047 as accession number. Specifically, 20 samples divided between NW (control), OBF (obese women), OBM (obese male) and OBT2D (obese women with diabetes) are deposited in the GSE166047. We hereby describe only 10 samples (5 healthy normal weight women reported as NW and 5 obese women reported as OBF) because we refer to the data published in the article "Transcriptional characterization of Subcutaneous Adipose Tissue in obesity affected women highlights metabolic dysfunction and implications for lncRNAs" (DOI: 10.1016/j.ygeno.2021.09.014). Pathways analyses were performed on g:Profiler, Enrichr, ClueGO and GSEA to gain biological insights on gene expression. Raw data reported in GEO database along with detailed methods description reported in this data article could be reused for comparisons with other datasets on the topic to obtain transcriptional differences in a wider co-hort. Moreover, detailed pathways analysis along with cross-referenced data with other datasets will allow to identify novel dysregulated pathways and genes responsible for this regulation. The biological interpretation of this dataset, along with related in vitro experiments, is reported by Rey et al., in Genomics (DOI: 10.1016/j.ygeno.2021.09.014). (C) 2021 Published by Elsevier Inc
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