Spin-dependent dark matter-electron interactions

Detectors with low thresholds for electron recoil open a new window to direct searches of sub-GeV dark matter (DM) candidates. In the past decade, many strong limits on DM-electron interactions have been set, but most on the one which is spin-independent (SI) of both dark matter and electron spins. In this work, we study DM-atom scattering through a spin-dependent (SD) interaction at leading order (LO), using well-benchmarked, state-of-the-art atomic many-body calculations. Exclusion limits on the SD DM-electron cross section are derived with data taken from experiments with xenon and germanium detectors at leading sensitivities. In the DM mass range of 0.1 - 10 GeV, the best limits set by the XENON1T experiment: $\sigma_e^{\textrm{(SD)}}<10^{-41}-10^{-40}\,\textrm{cm}^2$ are comparable to the ones drawn on DM-neutron and DM-proton at slightly bigger DM masses. The detector's responses to the LO SD and SI interactions are analyzed. In nonrelativistic limit, a constant ratio between them leads to an indistinguishability of the SD and SI recoil energy spectra. Relativistic calculations however show the scaling starts to break down at a few hundreds of eV, where spin-orbit effects become sizable. We discuss the prospects of disentangling the SI and SD components in DM-electron interactions via spectral shape measurements, as well as having spin-sensitive experimental signatures without SI background.Comment: Data files and README are provided in the ancillary folde

Aspergillus spp., a versatile cell factory for enzymes and metabolites: Interventions through genome editing

Aspergillus sp. is widely distributed in nature and plays significant roles in the degradation of lignocellulose biomass and extensively used in bioprocess and fermentation technology and many species are also a generally regarded safe. Many of the Aspergillus species are established cell factories due to their inherent capacity in secreting large number of hydrolytic enzymes. With the advent of next generation genomic technologies and metabolic engineering technologies, the production potential of Aspergillus cell factory has improved over the years. Various genome editing tools has been developed for Aspergillus like engineered nucleases, zinc finger nucleases, TALEN and CRISPR-Cas9 system. Currently, the CRISPR/Cas9-based technique is extensively used to enhance the effectiveness of gene manipulation in model system Aspergillus nidulans and other strains like Aspergillus oryzae, Aspergillus niger and Aspergillus fumigatus. This review describes the recent developments of genome editing technologies in Aspergillus the synthesis of heterologous proteins and secondary metabolites in the Aspergillus species

Effect of weaning on milking behaviour and temperament of Murrah buffaloes

The study was conducted to compare the milking behaviour and temperament of Murrah buffaloes due to suckling and weaning at different ages. Initially, 42 recently calved buffaloes were selected and assigned alternately in to 3 groups as suckling (Gr 1), weaning at birth (Gr 2) and weaning on 45th day (Gr 3). The different behavioural parameters of the buffaloes were recorded for the first 15 days of milking/weaning during each milking. The overall average milk let down time was non-significant among the groups. The average milk yield per milking (morning and evening) was significantly (P<0.01) higher in Gr 1 (3.44±0.14 kg) and Gr 2 (3.18±0.46 kg) in comparison to Gr 3 (2.24±0.25 kg). The overall average milking time was significantly (P<0.01) higher in Gr 1 (4.41±0.14 min) than Gr 3 (3.05±0.21 min); however milking time in Gr 2 (4.29±0.41min) was not significantly different from Gr 1. The percent of dams showing stepping behaviour in Gr 1, Gr 2 and Gr 3 was 20.23, 27.57 and 44.11, respectively, and the corresponding value for kicking behaviour was 2.61, 10.60 and 13.39, respectively. The average number of dams showing bellowing behaviour in Gr 1, Gr 2 and Gr 3 was 3.56, 9.09 and 12.66, respectively. Similar trends were observed among the groups as mentioned in stepping and kicking. The percentage of dams which licked calf or looked at milker in Gr 1, Gr 2 and Gr 3 was 34.99, 9.38 and 33.33 respectively. The percentage of dams showing head shaking activity during milking operation in Gr 1, Gr 2 and Gr 3 was 26.42, 0.00 and 10.00 percent, respectively. The overall mean milking temperament score was significantly (P<0.05) higher in Gr 3 (1.73±0.18) than Gr 1 (1.20±0.05) and Gr 2 (1.48±0.17). The success rate of weaning practice in dairy buffaloes was higher at calving than later stage of lactation

Synthesis of macromolecular systems via lipase catalyzed biocatalytic reactions: applications and future perspectives

Enzymes, being remarkable catalysts, are capable of accepting a wide range of complex molecules as substrates and catalyze a variety of reactions with a high degree of chemo-, stereo- and regioselectivity in most of the reactions. Biocatalysis can be used in both simple and complex chemical transformations without the need for tedious protection and deprotection chemistry that is very common in traditional organic synthesis. This current review highlights the applicability of one class of biocatalysts viz. ‘‘lipases’’ in synthetic transformations, the resolution of pharmaceutically important small molecules including polyphenols, amides, nucleosides and their precursors, the development of macromolecular systems (and their applications as drug/gene carriers), flame retardants, polymeric antioxidants and nanocrystalline solar cells, etc

NGS QC Toolkit: A Toolkit for Quality Control of Next Generation Sequencing Data

Next generation sequencing (NGS) technologies provide a high-throughput means to generate large amount of sequence data. However, quality control (QC) of sequence data generated from these technologies is extremely important for meaningful downstream analysis. Further, highly efficient and fast processing tools are required to handle the large volume of datasets. Here, we have developed an application, NGS QC Toolkit, for quality check and filtering of high-quality data. This toolkit is a standalone and open source application freely available at http://www.nipgr.res.in/ngsqctoolkit.html. All the tools in the application have been implemented in Perl programming language. The toolkit is comprised of user-friendly tools for QC of sequencing data generated using Roche 454 and Illumina platforms, and additional tools to aid QC (sequence format converter and trimming tools) and analysis (statistics tools). A variety of options have been provided to facilitate the QC at user-defined parameters. The toolkit is expected to be very useful for the QC of NGS data to facilitate better downstream analysis

Cloning, function, and localization of human, canine, and Drosophila ZIP10 (SLC39A10), a Zn2+ transporter

Zinc (Zn2+) is the second most abundant trace element, but is considered a micronutrient, as it is a cofactor for many enzymes and transcription factors. Whereas Zn2+ deficiency can cause cognitive immune or metabolic dysfunction and infertility, excess Zn2+ is nephrotoxic. As for other ions and solutes, Zn2+ is moved into and out of cells by specific membrane transporters: ZnT, Zip, and NRAMP/DMT proteins. ZIP10 is reported to be localized at the apical membrane of renal proximal tubules in rats, where it is believed to play a role in Zn2+ import. Renal regulation of Zn2+ is of particular interest in light of growing evidence that Zn2+ may play a role in kidney stone formation. The objective of this study was to show that ZIP10 homologs transport Zn2+, as well as ZIP10, kidney localization across species. We cloned ZIP10 from dog, human, and Drosophila (CG10006), tested clones for Zn2+ uptake in Xenopus oocytes and localized the protein in renal structures. CG10006, rather than foi (fear-of-intimacy, CG6817) is the primary ZIP10 homolog found in Drosophila Malpighian tubules. The ZIP10 antibody recognizes recombinant dog, human, and Drosophila ZIP10 proteins. Immunohistochemistry reveals that ZIP10 in higher mammals is found not only in the proximal tubule, but also in the collecting duct system. These ZIP10 proteins show Zn2+ transport. Together, these studies reveal ZIP10 kidney localization, a role in renal Zn2+ transport, and indicates that CG10006 is a Drosophila homolog of ZIP10