10 research outputs found
Electronic supplementary material Appendix S1 from Genome-wide single nucleotide polymorphism scan suggests adaptation to urbanization in an important pollinator, the red-tailed bumblebee (<i>Bombus lapidarius</i> L.)
Urbanization is considered a global threat to biodiversity; the growth of cities results in an increase in impervious surfaces, soil and air pollution, fragmentation of natural vegetation and invasion of non-native species along with numerous environmental changes, including the heat island phenomenon. The combination of these effects constitutes a challenge for both the survival and persistence of many native species, while also imposing altered selective regimes. Here, using 110 314 single nucleotide polymorphisms generated by restriction site-associated DNA sequencing, we investigated the genome-wide effects of urbanization on putative neutral and adaptive genomic diversity in a major insect pollinator, <i>Bombus lapidarius</i>, collected from nine German cities and nine paired rural sites. Overall, genetic differentiation among sites was low and there was no obvious genome-wide genetic structuring, suggesting the absence of strong effects of urbanization on gene flow. We, nevertheless, identified several loci under directional selection, a subset of which was associated with urban land use, including the percentage of impervious surface, surrounding each sampling site. Overall, our results provide evidence of local adaptation to urbanization in the face of gene flow in a highly mobile insect pollinator
Electronic supplementary material tables and figures from Genome-wide single nucleotide polymorphism scan suggests adaptation to urbanization in an important pollinator, the red-tailed bumblebee (<i>Bombus lapidarius</i> L.)
Urbanization is considered a global threat to biodiversity; the growth of cities results in an increase in impervious surfaces, soil and air pollution, fragmentation of natural vegetation and invasion of non-native species along with numerous environmental changes, including the heat island phenomenon. The combination of these effects constitutes a challenge for both the survival and persistence of many native species, while also imposing altered selective regimes. Here, using 110 314 single nucleotide polymorphisms generated by restriction site-associated DNA sequencing, we investigated the genome-wide effects of urbanization on putative neutral and adaptive genomic diversity in a major insect pollinator, <i>Bombus lapidarius</i>, collected from nine German cities and nine paired rural sites. Overall, genetic differentiation among sites was low and there was no obvious genome-wide genetic structuring, suggesting the absence of strong effects of urbanization on gene flow. We, nevertheless, identified several loci under directional selection, a subset of which was associated with urban land use, including the percentage of impervious surface, surrounding each sampling site. Overall, our results provide evidence of local adaptation to urbanization in the face of gene flow in a highly mobile insect pollinator
Sequences and trees
Tree files in newick format for BQCV and SBPV; raw cloned sequences in fasta format for BQCV, SBPV and ABP
Misc statistics
R code script and data for true prevalence estimates, tests of proportions and kolmogorov-smirnov test
GLMM and GLM models
Includes raw MLPA data files, R scripts and outpu
Script for CCA analysis and visualization
File with the script for execute the CCA analysis and visualize the result
Species
abundance and identity of species for the CCA analysi
data for SEM all
Data containing landscape proportion and abundance and richness (Chao-1 corrected) for the most abundant bees and for transect, pan traps and total dataset