Guarding Embryo Development of Zebrafish by Shell Engineering: A Strategy to Shield Life from Ozone Depletion

Background: The reduced concentration of stratospheric ozone results in an increased flux of biologically damaging midultraviolet radiation (UVB, 280 to 320 nm) reaching earth surfaces. Environmentally relevant levels of UVB negatively impact various natural populations of marine organisms, which is ascribed to suppressed embryonic development by increased radiation. Methodology/Principal Findings: Inspired by strategies in the living systems generated by evolution, we induce an extra UVB-adsorbed coat on the chorion (eggshell surrounding embryo) of zebrafish, during the blastula period. Short and long UV exposure experiments show that the artificial mineral-shell reduces the UV radiation effectively and the enclosed embryos become more robust. In contrast, the uncoated embryos cannot survive under the enhanced UVB condition. Conclusions: We suggest that an engineered shell of functional materials onto biological units can be developed as a strategy to shield lives to counteract negative changes of global environment, or to provide extra protection for the living units in biological research

Linguistic Focus Promotes the Ease of Discourse Integration Processes in Reading Comprehension: Evidence From Event-Related Potentials

Psycholinguistic studies of focus processing have yielded varying results regarding how focus affects language processing. We report the results of an event-related potential (ERP) experiment that used question-answer pairs in a discourse to manipulate whether a target word was contextually focused, contrastively focused, contextually defocused, or contextually neutral. We found a negative-going waveform that was sustained in the time-course (250–800 ms after the target word onset) with a maximum over frontal-central scalp sites. As the structure of the discourse made the target word more focused, the negative-going deflection was systematically reduced. We also observed a frontal positive-going waveform that was larger for the focus-marked words relative to the neutral target word in an earlier time window (150–250 ms, P200), which may reflect increased attention allocated to the focused items. We propose that the reduced negative ERPs for the focused words reflects facilitation of meaning integration when focus functions to establish reference in the discourse representation. This can be attributed to extra attention paid to the focus-marked items that in turn promotes the prominence of focus-marked referent and prompts the contextual priming mechanism that facilitates the access of propositionally relevant items in text memory during reading

DDX5 facilitates HIV-1 replication as a cellular co-factor of Rev.

HIV-1 Rev plays an important role in the late phase of HIV-1 replication, which facilitates export of unspliced viral mRNAs from the nucleus to cytoplasm in infected cells. Recent studies have shown that DDX1 and DDX3 are co-factors of Rev for the export of HIV-1 transcripts. In this report, we have demonstrated that DDX5 (p68), which is a multifunctional DEAD-box RNA helicase, functions as a new cellular co-factor of HIV-1 Rev. We found that DDX5 affects Rev function through the Rev-RRE axis and subsequently enhances HIV-1 replication. Confocal microscopy and co-immunoprecipitation analysis indicated that DDX5 binds to Rev and this interaction is largely dependent on RNA. If the DEAD-box motif of DDX5 is mutated, DDX5 loses almost all of its ability to bind to Rev, indicating that the DEAD-box motif of DDX5 is required for the interaction between DDX5 and Rev. Our data indicate that interference of DDX5-Rev interaction could reduce HIV-1 replication and potentially provide a new molecular target for anti-HIV-1 therapeutics

SkyMath: Technical Report

Large language models (LLMs) have shown great potential to solve varieties of natural language processing (NLP) tasks, including mathematical reasoning. In this work, we present SkyMath, a large language model for mathematics with 13 billion parameters. By applying self-compare fine-tuning, we have enhanced mathematical reasoning abilities of Skywork-13B-Base remarkably. On GSM8K, SkyMath outperforms all known open-source models of similar size and has established a new SOTA performance

Für | For Manfred from his Students

Dieses Buch enthält Beiträge von Personen, die ihre Magister- oder Doktorarbeit unter der Betreuung von Manfred Krifka geschrieben haben. Es ist als kleines Abschiedsgeschenk für Manfred Krifka zum Ende seiner Amtszeit als Direktor des Leibniz-Zentrums für Allgemeine Sprachwissenschaft gedacht. Die Herausgeberin und der Herausgeber haben Beiträge zu sprachwissenschaftlichen und nicht-sprachwissenschaftlichen Themen in einer Vielzahl von Genres gesammelt. Diese Vielfalt spiegelt die Interessen und Forschungsthemen von Manfred Krifka wider. Sie spiegelt auch die Vielfalt der Menschen wider, denen Manfred Krifka geholfen hat