IN VITRO TECHNIQUES FOR ANALYZING THE PHARMACOKINETIC CHARACTERISTICS OF BIOACTIVE COMPOUNDS DERIVED FROM PLANTS: A SYSTEMATIC REVIEW

Natural products are the main materials of studies in which they are manufactured, mainly the raw materials of studies of the companies, due to the many numbers of compounds that are manufactured to all studies of factories, mainly of studies manufactured in large numbers of plants , with most plant studies presented to many vegetables. Seen, the general objective of the study is to know the pharmacokinetic techniques and in vitro investigation methods applied in compositions with bioactive research results. In order to fulfill the proposal, a survey of scientific articles will be carried out in international databases, containing the main methods of investigation of the main kinetic characteristics in vitro, tracing the profile of bioactive compounds with plants of plants. Furthermore, an assessment of the methodological quality and risk of bias of the selected studies will be carried out. The review question consists of: What are the techniques used for pharmacokinetic characterization of natural products investigated by in vitro assays? In this way, a review will be prepared according to the PICOS strategy, which briefly addresses P= problem; I= intervention; C= control; O= determination; S= type of study. Contractors were determined from cyclopedia queries of terms controlled by the National Library of Medicine's Medical Subject Headings (MeSH) control and by the diversity of options and trilin National Library of Medicine (Health Descriptors). Thus, for literature search, three electronic databases will be used: PubMed, Scopus and Web of Science. The selection of selected studies will be considered as a result of two independent experts, by an evaluator study, with the studies reviewed and will be evaluated using the abstracts and abstracts application, with the use of the Rayyan abstracts application, with clear criteria of inclusion and exclusion. Finally, extremely important study projects, given the study in search of applied techniques for pharmacocin of bioactive compounds resulting from plants, develop technical procedures with new methods of quality, safety and analysis of studies, as well as future quality procedures

Evaluation of a quality improvement intervention to reduce anastomotic leak following right colectomy (EAGLE): pragmatic, batched stepped-wedge, cluster-randomized trial in 64 countries

Background Anastomotic leak affects 8 per cent of patients after right colectomy with a 10-fold increased risk of postoperative death. The EAGLE study aimed to develop and test whether an international, standardized quality improvement intervention could reduce anastomotic leaks. Methods The internationally intended protocol, iteratively co-developed by a multistage Delphi process, comprised an online educational module introducing risk stratification, an intraoperative checklist, and harmonized surgical techniques. Clusters (hospital teams) were randomized to one of three arms with varied sequences of intervention/data collection by a derived stepped-wedge batch design (at least 18 hospital teams per batch). Patients were blinded to the study allocation. Low- and middle-income country enrolment was encouraged. The primary outcome (assessed by intention to treat) was anastomotic leak rate, and subgroup analyses by module completion (at least 80 per cent of surgeons, high engagement; less than 50 per cent, low engagement) were preplanned. Results A total 355 hospital teams registered, with 332 from 64 countries (39.2 per cent low and middle income) included in the final analysis. The online modules were completed by half of the surgeons (2143 of 4411). The primary analysis included 3039 of the 3268 patients recruited (206 patients had no anastomosis and 23 were lost to follow-up), with anastomotic leaks arising before and after the intervention in 10.1 and 9.6 per cent respectively (adjusted OR 0.87, 95 per cent c.i. 0.59 to 1.30; P = 0.498). The proportion of surgeons completing the educational modules was an influence: the leak rate decreased from 12.2 per cent (61 of 500) before intervention to 5.1 per cent (24 of 473) after intervention in high-engagement centres (adjusted OR 0.36, 0.20 to 0.64; P < 0.001), but this was not observed in low-engagement hospitals (8.3 per cent (59 of 714) and 13.8 per cent (61 of 443) respectively; adjusted OR 2.09, 1.31 to 3.31). Conclusion Completion of globally available digital training by engaged teams can alter anastomotic leak rates. Registration number: NCT04270721 (http://www.clinicaltrials.gov)