Risk communication and community right to know: A public relations obligation to inform

Risk communication and community right to know are increasingly important functions of public relations within communities that face a considerable amount of health, safety and environmental risk related to chemical manufacturing. Following Susan G. Hadden’s (1989) claim that community right to know is not only a legal subject but a powerful approach to risk communication and by extension public relations, this research project analyzed, through an ethnographic case study of participant observations (n=193 days), interviews (n=27) and focus groups (n=15), how community residents perceive and construct their awareness and understanding of significant federally mandated and industry initiated community-right-to-know initiatives within risk communication. Findings include a general lack of awareness and understanding of community-right-to-know programs and risk management protocols, with differences among communities, cultures and genders, and the social justification of risks via narratives

A Study of West Virginia Elementary Special Education Teachers\u27 Roles, Responsibilities, and Practices within a Multitiered Instructional Support System: Implications for Policy and Practice

The purpose of the study was to provide a data-based description of West Virginia special education teachers’ roles, responsibilities, and practices relevant to their participation in selected components of the Response to Intervention (RTI) process. Special educators’ practices related to assessment, tiered instruction, decision making, and collaboration comprise the main components of study and were based on a conceptual framework developed by Hoover and Patton (2008). A researcher-developed survey, the Special Educator Response to Intervention Inventory (SERTII), was used to explore the extent and characteristics of special educators’ participation in RTI. The study population included all elementary special education teachers in West Virginia. The sample for the study included 341 special educators. Statistical analyses showed significant levels of participation across each of the four areas examined. Elementary special educators participated in progress monitoring, provided weekly intervention sessions to at-risk students, and used research-based, explicit instructional strategies when delivering intervention. Teachers analyzed data and consulted regularly with general educators. They reported significant increases in the amount of collaboration between general and special educators since the initiation of RTI. Special education teachers participated in RTI decision-making activities such as determining students’ needs for intervention, selecting and developing interventions, and making referrals for special education evaluations. Self-reported qualitative data revealed special educators identify benefits of RTI as offering additional assistance to at-risk students, meeting individual needs, and delivering early intervention. Most commonly noted challenges of RTI for special educators included having sufficient time to implement the process, meeting the simultaneous needs of at-risk and IEP students, and interference with timely referrals for special education evaluation

Another part of the risk communicatin model: Analysis of risk communication process and message content

The authors undertook a study to define the messages that exist in 2 communities of risk (e.g., high concentration of chemical facilities) using the principles of fantasy theme analysis and symbolic convergence theory. Through several methodological steps including a document review, interviews, focus groups, and a telephone survey (N = 450), the researchers determined the messages that dominate in the community, and were able to segment them into rhetorical visions based on master analogues. Analysis indicated that persons who adhere to different perspectives or opinions (measured as rhetorical visions) experience different amounts of uncertainty, control, and support or opposition for the industries that create the risks. This analysis adds depth to the risk communication literature and suggests that public relations practitioners can and should attempt to understand risk discourse content as well as the communication processes and risk perceptions held by key publics

Emergency Text Messaging Systems and Higher Education Campuses: Expanding Crisis Communication and Chaos Theory

Recent public safety threats affecting college and university campuses during episodes of natural disasters and mass violence have exposed numerous challenges and opportunities in risk and crisis communication. This study addresses how colleges and universities have incorporated emergency text messaging systems into their crisis communication plans; how these institutions have tested such emergency notification systems; and what, if any, prevalent gaps exist between audience expectations and actual practices. Using grounded theory, the data collected in this study through in-depth phone interviews (N=10) of university public relations practitioners, as well as a document analysis of media coverage of campus crises (N=36), offered a humanistic and constructivist perspective about circumstances related to emergency text message alert systems that few researchers have explored. The analysis of the data also revealed and confirmed that chaos theory can play a role as a significant theory and potentially guiding paradigm of crisis communications research

Genome-wide patterns of carbon and nitrogen regulation of gene expression validate the combined carbon and nitrogen (CN)-signaling hypothesis in plants

BACKGROUND: Carbon and nitrogen are two signals that influence plant growth and development. It is known that carbon- and nitrogen-signaling pathways influence one another to affect gene expression, but little is known about which genes are regulated by interactions between carbon and nitrogen signaling or the mechanisms by which the different pathways interact. RESULTS: Microarray analysis was used to study global changes in mRNA levels due to carbon and nitrogen in Arabidopsis thaliana. An informatic analysis using InterAct Class enabled us to classify genes on the basis of their responses to carbon or nitrogen treatments. This analysis provides in vivo evidence supporting the hypothesis that plants have a carbon/nitrogen (CN)-sensing/regulatory mechanism, as we have identified over 300 genes whose response to combined CN treatment is different from that expected from expression values due to carbon and nitrogen treatments separately. Metabolism, energy and protein synthesis were found to be significantly affected by interactions between carbon and nitrogen signaling. Identified putative cis-acting regulatory elements involved in mediating CN-responsive gene expression suggest multiple mechanisms for CN responsiveness. One mechanism invokes the existence of a single CN-responsive cis element, while another invokes the existence of cis elements that promote nitrogen-responsive gene expression only when present in combination with a carbon-responsive cis element. CONCLUSION: This study has allowed us to identify genes and processes regulated by interactions between carbon and nitrogen signaling and take a first step in uncovering how carbon- and nitrogen-signaling pathways interact to regulate transcription

Genome-wide investigation of light and carbon signaling interactions in Arabidopsis

BACKGROUND: Light and carbon are two essential signals influencing plant growth and development. Little is known about how carbon and light signaling pathways intersect or influence one another to affect gene expression. RESULTS: Microarrays are used to investigate carbon and light signaling interactions at a genome-wide level in Arabidopsis thaliana. A classification system, 'InterAct Class', is used to classify genes on the basis of their expression profiles. InterAct classes and the genes within them are placed into theoretical models describing interactions between carbon and light signaling. Within InterAct classes there are genes regulated by carbon (201 genes), light (77 genes) or through carbon and light interactions (1,247 genes). We determined whether genes involved in specific biological processes are over-represented in the population of genes regulated by carbon and/or light signaling. Of 29 primary functional categories identified by the Munich Information Center for Protein Sequences, five show over-representation of genes regulated by carbon and/or light. Metabolism has the highest representation of genes regulated by carbon and light interactions and includes the secondary functional categories of carbon-containing-compound/carbohydrate metabolism, amino-acid metabolism, lipid metabolism, fatty-acid metabolism and isoprenoid metabolism. Genes that share a similar InterAct class expression profile and are involved in the same biological process are used to identify putative cis elements possibly involved in responses to both carbon and light signals. CONCLUSIONS: The work presented here represents a method to organize and classify microarray datasets, enabling one to investigate signaling interactions and to identify putative cis elements in silico through the analysis of genes that share a similar expression profile and biological function

Expansion of the Knockdown Resistance Frequency Map for Human Head Lice (Phthiraptera: Pediculidae) in the United States Using Quantitative Sequencing

Pediculosis is a prevalent parasitic infestation of humans, which is increasing due, in part, to the selection of lice resistant to either the pyrethrins or pyrethroid insecticides by the knockdown resistance (kdr) mechanism. To determine the extent and magnitude of thekdr-type mutations responsible for this resistance, lice were collected from 138 collection sites in 48 U.S. states from 22 July 2013 to 11 May 2015 and analyzed by quantitative sequencing. Previously published data were used for comparisons of the changes in the frequency of thekdr-type mutations over time. Mean percent resistance allele frequency (mean % RAF) values across the three mutation loci were determined from each collection site. The overall mean % RAF (+/-SD) for all analyzed lice was 98.3 +/- 10%. 132/138 sites (95.6%) had a mean % RAF of 100%, five sites (3.7%) had intermediate values, and only a single site had no mutations (0.0%). Forty-two states (88%) had a mean % RAF of 100%. The frequencies ofkdr-type mutations did not differ regardless of the human population size that the lice were collected from, indicating a uniformly high level of resistant alleles. The loss of efficacy of the Nix formulation (Prestige Brand, Tarrytown, NY) from 1998 to 2013 was correlated to the increase inkdr-type mutations. These data provide a plausible reason for the decrease in the effectiveness of permethrin in the Nix formulation, which is the parallel increase ofkdr-type mutations in lice over time

Quantitative Sequencing for the Determination of Kdr-type Resistance Allele (V419L,L925I, I936F) Frequencies in Common Bed Bug, Cimex lectularius L., (Hemiptera: Cimicidae) Populations Collected from Israel

Human bed bug infestations have dramatically increased worldwide since the mid-1990s. A similar phenomenon was also observed in Israel since 2005, when infestations were reported from all over the country. Two single nucleotide polymorphisms (V419L and L925I) in the bed bug voltage sensitive sodium channel confer kdr-type resistance to pyrethroids. Using quantitative sequencing (QS), the resistance allele frequencies of Israeli bed bug populations from across the country were determined. Genomic DNA was extracted from samples of 12 populations of bed bugs collected from Israel and DNA fragments containing the V419L or L925I and I936F mutations sites were PCR amplified. The PCR products were analyzed by QS and the nucleotide signal ratios calculated and used to predict the resistance allele frequencies of the unknown populations. Results of the genetic analysis show that resistant nucleotide signals are highly correlated to resistance allele frequencies for both mutations. Ten of the 12 tested populations had 100% of the L925I mutation and 0% of the V419L mutation. One population was heterogeneous for the L925I mutation and had 0% of the V419L mutation and another population was heterozygous for the V419L mutation and had 100% of the L925I mutation. I936F occurred only at low levels. These results indicate that bed bugs in Israel are genetically resistant to pyrethroids. Thus, pyrethroids should only be used for bed bug management with caution using effective application and careful monitoring procedures. Additionally, new and novel-acting insecticides and non-chemical means of controlling bed bugs should be explored

Expressed sequence tag analysis in Cycas, the most primitive living seed plant

BACKGROUND: Cycads are ancient seed plants (living fossils) with origins in the Paleozoic. Cycads are sometimes considered a 'missing link' as they exhibit characteristics intermediate between vascular non-seed plants and the more derived seed plants. Cycads have also been implicated as the source of 'Guam's dementia', possibly due to the production of S(+)-beta-methyl-alpha, beta-diaminopropionic acid (BMAA), which is an agonist of animal glutamate receptors. RESULTS: A total of 4,200 expressed sequence tags (ESTs) were created from Cycas rumphii and clustered into 2,458 contigs, of which 1,764 had low-stringency BLAST similarity to other plant genes. Among those cycad contigs with similarity to plant genes, 1,718 cycad 'hits' are to angiosperms, 1,310 match genes in gymnosperms and 734 match lower (non-seed) plants. Forty-six contigs were found that matched only genes in lower plants and gymnosperms. Upon obtaining the complete sequence from the clones of 37/46 contigs, 14 still matched only gymnosperms. Among those cycad contigs common to higher plants, ESTs were discovered that correspond to those involved in development and signaling in present-day flowering plants. We purified a cycad EST for a glutamate receptor (GLR)-like gene, as well as ESTs potentially involved in the synthesis of the GLR agonist BMAA. CONCLUSIONS: Analysis of cycad ESTs has uncovered conserved and potentially novel genes. Furthermore, the presence of a glutamate receptor agonist, as well as a glutamate receptor-like gene in cycads, supports the hypothesis that such neuroactive plant products are not merely herbivore deterrents but may also serve a role in plant signaling