Biochemical Evaluation of Withania Somnifera Root Powder on Adjuvant-Induced Arthritis in Rats

The present investigation was carried out to evaluate the biochemical effect of Withania somnifera Linn. Solanaceae, commonly known as ashwagandha on adjuvant induced arthritic rats. Results were compared to Indomethacin, a non steroidal anti-inflammatory drug. Arthritis was induced by an intra dermal injection of Complete Freund’s Adjuvant (0.1 ml) into the right hind paw of Wistar albino rats. Withania somnifera root powder (1000 mg/kg/day) and Indomethacin (3 mg/kg/day) were orally administered for 8 days (from 11th to 18th day) after adjuvant injection. After the experimental period, all the animals were sacrificed and serum, liver and spleen samples were collected for further biochemical analysis. A significant increase in the activities of gluconeogenic enzymes, tissue marker enzymes, blood glucose level, WBC, platelet count, erythrocyte sedimentation rate, and acute phase proteins (hyaluronic acid, fibrinogen and ceruloplasmin) was observed in adjuvant-induced arthritic rats, whereas the activities of glycolytic enzymes, body weight, levels of hemoglobin, RBC count, and packed cell volume were found to be decreased. These biochemical alterations observed in arthritic animals were ameliorated significantly after the administration of Withania somnifera root powder (1000 mg/kg/b.wt) and Indomethacin (3 mg/kg/b.wt). Our results suggest that Withania somnifera root powder is capable of rectifying the above biochemical changes in adjuvant arthritis and it may prove to be useful in treating rheumatoid arthritis

Biochemical Evaluation of Withania somnifera Root Powder on Adjuvant-Induced Arthritis in Rats

The present investigation was carried out to evaluate the biochemical effect of Withania somnifera Linn. Solanaceae, commonly known as ashwagandha on adjuvant induced arthritic rats. Results were compared to Indomethacin, a non steroidal anti-inflammatory drug. Arthritis was induced by an intra dermal injection of Complete Freund's Adjuvant (0.1 ml) into the right hind paw of Wistar albino rats. Withania somnifera root powder (1000 mg/kg/day) and Indomethacin (3 mg/kg/day) were orally administered for 8 days (from 11th to 18th day) after adjuvant injection. After the experimental period, all the animals were sacrificed and serum, liver and spleen samples were collected for further biochemical analysis. A significant increase in the activities of gluconeogenic enzymes, tissue marker enzymes, blood glucose level, WBC, platelet count, erythrocyte sedimentation rate, and acute phase proteins (hyaluronic acid, fibrinogen and ceruloplasmin) was observed in adjuvant-induced arthritic rats, whereas the activities of glycolytic enzymes, body weight, levels of hemoglobin, RBC count, and packed cell volume were found to be decreased. These biochemical alterations observed in arthritic animals were ameliorated significantly after the administration of Withania somnifera root powder (1000 mg/kg/b.wt) and Indomethacin (3 mg/kg/b.wt). Our results suggest that Withania somnifera root powder is capable of rectifying the above biochemical changes in adjuvant arthritis and it may prove to be useful in treating rheumatoid arthritis

Biochemical Evaluation of Withania somnifera Root Powder on Adjuvant-Induced Arthritis in Rats

The present investigation was carried out to evaluate the biochemical effect of Withania somnifera Linn. Solanaceae, commonly known as ashwagandha on adjuvant induced arthritic rats. Results were compared to Indomethacin, a non steroidal anti-inflammatory drug. Arthritis was induced by an intra dermal injection of Complete Freund’s Adjuvant (0.1 ml) into the right hind paw of Wistar albino rats. Withania somnifera root powder (1000 mg/kg/day) and Indomethacin (3 mg/kg/day) were orally administered for 8 days (from 11th to 18th day) after adjuvant injection. After the experimental period, all the animals were sacrificed and serum, liver and spleen samples were collected for further biochemical analysis. A significant increase in the activities of gluconeogenic enzymes, tissue marker enzymes, blood glucose level, WBC, platelet count, erythrocyte sedimentation rate, and acute phase proteins (hyaluronic acid, fibrinogen and ceruloplasmin) was observed in adjuvant-induced arthritic rats, whereas the activities of glycolytic enzymes, body weight, levels of hemoglobin, RBC count, and packed cell volume were found to be decreased. These biochemical alterations observed in arthritic animals were ameliorated significantly after the administration of Withania somnifera root powder (1000 mg/kg/b.wt) and Indomethacin (3 mg/kg/b.wt). Our results suggest that Withania somnifera root powder is capable of rectifying the above biochemical changes in adjuvant arthritis and it may prove to be useful in treating rheumatoid arthritis

Role of sulphated polysaccharides from in Cyclosporine A-induced oxidative liver injury in rats-1

and Mg-ATPase: μmole of inorganic phosphorous liberated/min/mg protein. Group I – Control; Group II – CsA; Group III – Sulphated polysaccharides; Group IV – CsA+sulphated polysaccharides Comparisons are made between: a-Group I and Group II, III, IV; b-Group II and Group IV. The symbols (***), (**) and (*) represent statistical significance at < 0.001, < 0.01 and < 0.05, respectively.<p><b>Copyright information:</b></p><p>Taken from "Role of sulphated polysaccharides from in Cyclosporine A-induced oxidative liver injury in rats"</p><p>BMC Pharmacology 2008;8():4-4.</p><p>Published online 20 Feb 2008</p><p>PMCID:PMC2291455.</p><p></p

Histopathological findings in the liver tissue of CsA-induced and treated groups

Control and drug control groups show normal liver architecture (Figure 3a and 3c). Liver sections treated with CsA produces marked changes like inflammation around portal triad (Triaditis) with patchy microvesicular fatty degeneration (Figure 3b). Sulphated polysaccharides treated rats (Figure 3d) show considerable reduction in the pathological changes compared to CsA-induced animals.<p><b>Copyright information:</b></p><p>Taken from "Role of sulphated polysaccharides from in Cyclosporine A-induced oxidative liver injury in rats"</p><p>http://www.biomedcentral.com/1471-2210/8/4</p><p>BMC Pharmacology 2008;8():4-4.</p><p>Published online 20 Feb 2008</p><p>PMCID:PMC2291455.</p><p></p

Effect of CsA and sulphated polysaccharides on hepatic oxidants production

Values are expressed as mean ± S.D. for six animals in each group. Group I – Control; Group II – CsA; Group III – Sulphated polysaccharides; Group IV – CsA+sulphated polysaccharides. Comparisons are made between: a-Group I and Groups II, III, IV; b-Group II and Group IV. The symbols (***) and (**) represent statistical significance at < 0.001 and < 0.01, respectively.<p><b>Copyright information:</b></p><p>Taken from "Role of sulphated polysaccharides from in Cyclosporine A-induced oxidative liver injury in rats"</p><p>http://www.biomedcentral.com/1471-2210/8/4</p><p>BMC Pharmacology 2008;8():4-4.</p><p>Published online 20 Feb 2008</p><p>PMCID:PMC2291455.</p><p></p