8 research outputs found
Outbreak and control of haemorrhagic pneumonia due to Streptococcus equi subspecies zooepidemicus in dogs
This work was supported by the Brain Korea 21 Program for
Veterinary Science and the Korea Research Foundation (KRF-
2004-005-E00077)
Deepnglypred: A deep neural network-based approach for human n-linked glycosylation site prediction
Protein N-linked glycosylation is a post-translational modification that plays an important role in a myriad of biological processes. Computational prediction approaches serve as comple-mentary methods for the characterization of glycosylation sites. Most of the existing predictors for N-linked glycosylation utilize the information that the glycosylation site occurs at the N-X-[S/T] se-quon, where X is any amino acid except proline. Not all N-X-[S/T] sequons are glycosylated, thus the N-X-[S/T] sequon is a necessary but not sufficient determinant for protein glycosylation. In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem. Here, we report DeepNGlyPred a deep learning-based approach that encodes the positive and negative sequences in the human proteome dataset (extracted from N-GlycositeAtlas) using sequence-based features (gapped-dipeptide), predicted structural features, and evolutionary information. DeepNGlyPred produces SN, SP, MCC, and ACC of 88.62%, 73.92%, 60%, and 79.41%, respectively on N-GlyDE independent test set, which is better than the compared approaches. These results demonstrate that DeepNGlyPred is a robust computational technique to predict N-Linked glycosylation sites confined to N-X-[S/T] sequon. DeepNGlyPred will be a useful resource for the glycobiology community
LMPhosSite: A Deep Learning-Based Approach for General Protein Phosphorylation Site Prediction Using Embeddings from the Local Window Sequence and Pretrained Protein Language Model
Phosphorylation is one of the most important post-translational modifications and plays a pivotal role in various cellular processes. Although there exist several computational tools to predict phosphorylation sites, existing tools have not yet harnessed the knowledge distilled by pretrained protein language models. Herein, we present a novel deep learning-based approach called LMPhosSite for the general phosphorylation site prediction that integrates embeddings from the local window sequence and the contextualized embedding obtained using global (overall) protein sequence from a pretrained protein language model to improve the prediction performance. Thus, the LMPhosSite consists of two base-models: one for capturing effective local representation and the other for capturing global per-residue contextualized embedding from a pretrained protein language model. The output of these base-models is integrated using a score-level fusion approach. LMPhosSite achieves a precision, recall, Matthew\u27s correlation coefficient, and F1-score of 38.78%, 67.12%, 0.390, and 49.15%, for the combined serine and threonine independent test data set and 34.90%, 62.03%, 0.298, and 44.67%, respectively, for the tyrosine independent test data set, which is better than the compared approaches. These results demonstrate that LMPhosSite is a robust computational tool for the prediction of the general phosphorylation sites in proteins
LMPhosSite: A Deep Learning-Based Approach for General Protein Phosphorylation Site Prediction Using Embeddings from the Local Window Sequence and Pretrained Protein Language Model
Phosphorylation is one of the most important post-translational
modifications and plays a pivotal role in various cellular processes.
Although there exist several computational tools to predict phosphorylation
sites, existing tools have not yet harnessed the knowledge distilled
by pretrained protein language models. Herein, we present a novel
deep learning-based approach called LMPhosSite for the general phosphorylation
site prediction that integrates embeddings from the local window sequence
and the contextualized embedding obtained using global (overall) protein
sequence from a pretrained protein language model to improve the prediction
performance. Thus, the LMPhosSite consists of two base-models: one
for capturing effective local representation and the other for capturing
global per-residue contextualized embedding from a pretrained protein
language model. The output of these base-models is integrated using
a score-level fusion approach. LMPhosSite achieves a precision, recall,
Matthew's correlation coefficient, and F1-score of 38.78%, 67.12%,
0.390, and 49.15%, for the combined serine and threonine independent
test data set and 34.90%, 62.03%, 0.298, and 44.67%, respectively,
for the tyrosine independent test data set, which is better than the
compared approaches. These results demonstrate that LMPhosSite is
a robust computational tool for the prediction of the general phosphorylation
sites in proteins
Report on association of plant parasitic nematodes in large cardamom (Amomum subulatum Roxb.) at Sikkim, Himalaya region of India: Plant parasitic nematodes in large cardamom in Sikkim
Large cardamom (Amomum subulatum Roxb.), is a major cash crop grown in Sikkim Himalaya region. The crop is found to be affected by many pests (insects and diseases); which hinders the production, productivity as well as quality of the produce. Based on symptoms in this crop, similar to nematode infestation, soil and root samples were collected from the rhizosphere of different large cardamom fields and analyzed for presence of nematodes. Laboratory analysis revealed the association of six nematode species with large cardamom viz., Meloidogyne incognita, Helicotylenchus spp., Hoplolaimus sp., Tylenchorhynchus annulatus, Pratylenchus sp. and Macroposthonia spp., of which Meloidogyne incognita and Pratylenchus sp. were recorded in roots also and the percentage of occurance was 59.09. Apart from Meloidogyne incognita, all other five nematode species were recorded for the first time in large cardamom. Helicotylenchus spp. was the dominant genera among the six genera with 50% occurrence, 61.56 % relative abundance and 60 nematodes per 200 cc soil