11 research outputs found
Participation of glutamate dehydrogenase (GDH) in hetero-enzyme complexes:Relation to neurotransmitter homeostasis and brain energy metabolism
Characterization of primary and secondary cultures of astrocytes prepared from mouse cerebral cortex
Coupled lateral-longitudinal vehicle dynamics and control design with three-dimensional state portraits
Independent Wheel Effects in Real Time Estimation of Tire-Road Friction Coefficient from Steering Torque
Glutamate dehydrogenase Isoforms with N-Terminal (His)6- or FLAG-tag retain their kinetic properties and cellular localization
siRNA knock down of glutamate dehydrogenase in astrocytes affects glutamate metabolism leading to extensive accumulation of the neuroactive amino acids glutamate and aspartate
GDH-Dependent Glutamate Oxidation in the Brain Dictates Peripheral Energy Substrate Distribution
Glucose, the main energy substrate used in the CNS, is continuously supplied by the periphery. Glutamate, the major excitatory neurotransmitter, is foreseen as a complementary energy contributor in the brain. In particular, astrocytes actively take up glutamate and may use it through oxidative glutamate dehydrogenase (GDH) activity. Here, we investigated the significance of glutamate as energy substrate for the brain. Upon glutamate exposure, astrocytes generated ATP in a GDH-dependent way. The observed lack of glutamate oxidation in brain-specific GDH null CnsGlud1−/− mice resulted in a central energy-deprivation state with increased ADP/ATP ratios and phospho-AMPK in the hypothalamus. This induced changes in the autonomous nervous system balance, with increased sympathetic activity promoting hepatic glucose production and mobilization of substrates reshaping peripheral energy stores. Our data reveal the importance of glutamate as necessary energy substrate for the brain and the role of central GDH in the regulation of whole-body energy homeostasis