Online Learning Development 2021 as Skill Refresh Solution for Shell’s Gas Station Employee during the Covid-19 Pandemic

One form of investments a company can take is employee training. But it was constrained by the Covid-19 pandemic. Many companies have ended up having to temporarily suspend their employee training activities during this pandemic. But actually, this condition should not prevent the companies from carrying out their employee training programs. There are many training methods to choose, in this pandemic condition. And the online training is one of the methods to choose.This research aims to discover the implementation of the Online Learning Development 2021 as Skill Refresh Solution for Shell’s Gas Station Employee during the Covid-19 Pandemic. The research employed the descriptive qualitative research method. Data used in this research consist of primary and secondary data. The techniques for gathering data to answer the research problem are observations, questionnaires, interviews, and documentations.This research found that the Online Learning Development 2021 was an online work training held by PT. Shell. The purpose of the training is to develop the skills of competent gas station operators and to maintain the safety and also to decrease the rate of accidents at the gas stations. The participants that were involved in the training program are (1) Shift Managers; (2) Forecourt Service Champions (FSC); (3) Select Service Champions (SSC); and (4) mechanics. The Online Learning Development was implemented for a full year in 2021. The training materials were relevant to the current challenge, which is how to handle customer complaints, how to give satisfactory services to customers, good customer service principles, the usage technique of APAR for early fire extinguishing in gas stations, and the usage of dispensers and nozzles. Considering the risks at gas stations are still big, and the commitment in work safety and health, and also service quality, the training of human resources is something not to be ignored in order to sharpen the employee skills. Keywords: Online Learning Development, Shell gas stations, Covid-19 Pandemi

Um jeito gostoso e divertido de conhecer o leite.

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Severity of oxidative stress and inflammatory activation in end-stage heart failure patients are unaltered after 1 month of left ventricular mechanical assistance

This study investigates the impact of early left ventricular (LV)-mechanical unloading on systemic oxidative stress and inflammation in terminal heart failure patients and their impact both on multi organ failure and on intensive care unit (ICU) stay. Circulating levels of urinary 15-isoprostane-F2t (8-epi-PGF2a) and pro-inflammatory markers [plasma interleukin (IL)-6, IL-8, and urinary neopterin, a monocyte activation index] were analyzed in 20 healthy subjects, 22 stable end-stage heart failure (ESHF) patients and in 23 LV assist device (LVAD) recipients at pre-implant and during first post-LVAD (PL) month. Multiorgan function was evaluated by total Sequential Organ Failure Assessment (tSOFA) score. In LVAD recipients the levels of oxidative-inflammatory markers and tSOFA score were higher compared to other groups. After device implantation 8-epi-PGF2a levels were unchanged, while IL-6, and IL-8 levels increased during first week, and at 1 month returned to pre-implant values, while neopterin levels increased progressively during LVAD support. The tSOFA score worsened at 1 PL-week with respect to pre-implant value, but improved at 1 PL-month. The tSOFA score related with IL-6 and IL-8 levels, while length of ICU stay related with pre-implant IL-6 levels. These data suggest that hemodynamic instability in terminal HF is associated to worsening of systemic inflammatory and oxidative milieu that do not improve in the early phase of hemodynamic recovery and LV-unloading by LVAD, affecting multi-organ function and length of ICU stay. This data stimulate to evaluate the impact of inflammatory signals on long-term outcome of mechanical circulatory support

Association of pre-operative interleukin-6 levels with Interagency Registry for Mechanically Assisted Circulatory Support profiles and intensive care unit stay in left ventricular assist device patients

BACKGROUND: Inflammatory mechanisms are associated with worse prognosis in end-stage heart failure (ESHF) patients who require left ventricular assist device (LVAD) support. Interagency Registry for Mechanically Assisted Circulatory Support (INTERMACS) profiles describe patient condition at pre-implant and outcome. This study assessed the relationship among inflammation patterns and INTERMACS profiles in LVAD recipients. METHOD: Thirty ESHF patients undergoing LVAD implantation as bridge to transplant were enrolled. Blood and urine samples were collected pre-operatively and serially up to 2 weeks post-operatively for assessment of inflammatory markers (plasma levels of interleukin [IL]-6, IL-8, IL-10, and osteopontin, a cardiac inflammatory-remodeling marker; and the urine neopterin/creatinine ratio, a monocyte activation marker). Multiorgan function was evaluated by the total sequential organ failure assessment (tSOFA) score. Outcomes of interest were early survival, post-LVAD tSOFA score, and intensive care unit (ICU) length of stay. RESULTS: Fifteen patients had INTERMACS profiles 1 or 2 (Group A), and 15 had profiles 3 or 4 (Group B). At pre-implant, only IL-6 levels and the IL-6/IL-10 ratio were higher in Group A vs B. After LVAD implantation, neopterin/creatinine ratio and IL-8 levels increased more in Group A vs B. Osteopontin levels increased significantly only in Group B. The tSOFA score at 2 weeks post-LVAD and ICU duration were related with pre-implant IL-6 levels. CONCLUSIONS: The INTERMACS profiles reflect the severity of the pre-operative inflammatory activation and the post-implant inflammatory response, affecting post-operative tSOFA score and ICU stay. Therefore, inflammation may contribute to poor outcome in patients with severe INTERMACS profile

The case report of a 31-year-old man with giant cell myocarditis successfully treated with combined immunosuppression and veno-arterial extracorporeal membrane oxygenation for 21 days

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The Extra-Membranous Domains of the Competence Protein HofQ Show DNA Binding, Flexibility and a Shared Fold with Type I KH Domains

Secretins form large oligomeric assemblies in the membrane that control both macromolecular secretion and uptake. Several Pasteurellaceae are naturally competent for transformation, but the mechanism for DNA assimilation is largely unknown. In Haemophilus influenzae, the secretin ComE has been demonstrated to be essential for DNA uptake. In closely related Aggregatibacter actinomycetemcomitans, an opportunistic pathogen in periodontitis, the ComE homolog HofQ is believed to be the outer membrane DNA translocase. Here, we report the structure of the extramembranous domains of HofQ at 2.3 å resolution by X-ray crystallography. We also show that the extra-membranous domains of HofQ are capable of DNA binding. The structure reveals two secretin-like folds, the first of which is formed by means of a domain swap. The second domain displays extensive structural similarity to K homology (KH) domains, including the presence of a GxxG motif, which is essential for the nucleotide-binding function of KH domains, suggesting a possible mechanism for DNA binding by HofQ. The data indicate a direct involvement in DNA acquisition and provide insight into the molecular basis for natural competence. <br /

Avaliação sistemática do grau de satisfação dos clientes da Embrapa.

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Polymer microparticles with defined surface chemistry and topography mediate the formation of stem cell aggregates and cardiomyocyte function

Surface-functionalized microparticles are relevant to fields spanning engineering and biomedicine, with uses ranging from cell culture to advanced cell delivery. Varying topographies of biomaterial surfaces are also being investigated as mediators of cell-material interactions and subsequent cell fate. To investigate competing or synergistic effects of chemistry and topography in three-dimensional (3D) cell cultures, methods are required to introduce these onto microparticles without modification of their underlying mor-phology or bulk properties. In this study, a new approach for surface functionalization of poly(lactic acid) (PLA) microparticles is reported that allows decoration of the outer shell of the polyesters with additional polymers via aqueous atom transfer radical polymerization (ATRP) routes. PLA microparticles with smooth or dimpled surfaces were functionalized with poly(poly(ethylene glycol) methacrylate) (pPEG-MA) and poly[N-(3-aminopropyl)methacrylamide] (pAPMA) brushes, chosen for their potential abilities to mediate cell adhesion. X-ray Photoelectron Spectroscopy (XPS) and Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) analysis indicated homogeneous coverage of the microparticles with pol-ymer brushes while maintaining the original topographies. These materials were used to investigate the relative importance of surface chemistry and topography both on the formation of human immortalized mesenchymal stem cell (hiMSCs) particle-cell aggregates and on the enhanced contractility of cardiomyo-cytes derived from human induced pluripotent stem cells (hiPSC-CMs). The influence of surface chemis-try was found to be more important on the size of particle-cell aggregates than topographies. In addition, surface chemistries that best promoted hiMSC attachment also improved hiPSC-CM attachment and con-tractility. These studies demonstrated a new route to obtain topo-chemical combinations on polyester-based biomaterials, and provided clear evidence for the predominant effect of surface functionality over micron-scale dimpled topography in cell-microparticle interactions. These findings thus provide new guiding principles for the design of biomaterial interfaces to direct cell function

Heavy Metal Concentrations in Dairy Products from Sheep Milk Collected in Two Regions of Southern Italy

The aim of this work was to detect the concentrations of some heavy metals in milk collected from ewes in 8 farms located in Calabria and Campania and to evaluate to what extent these metals may be present in dairy products for human consumption. The analysis of chromium, cadmium, lead and mercury was performed in a atomic absorption spectrophotometer equipped with a graphite furnace. The determination of Hg content in dry samples was carried out by means of an automatic Mercury analyser. Chromium was the metal detected at highest levels in milk and lead was highest in fresh, mature cheese and in ricotta. In Italy, human consumption of sheep milk is very limited and addressed to milk products. In our study the levels of some metals were higher than those reported in literature. However, the results indicate that sheep milk and milk products from the two regions of Italy investigated in this study are safe for consumers