Antimicrobial activities of various essential oils against foodborne pathogenic or spoilage moulds

The use of essential oils in the food industry, as natural sanitizing agents, requires the definition of optimal conditions. The aim of the present work was to evaluate some antimicrobial activity parameters as mycelial growth inhibition, minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of six essential oils against Aspergillus niger, Aspergillus terreus,Chaetomium globosum, Penicillium chrysogenum, Penicillium pinophilum, Trichoderma harzianum and Trichoderma viride. The antimicrobial activity of essential oils was monitored by the macrodiluition technique. The mycelial growth inhibition, fungistatic and fungicidal concentrations were recorded for each strain that showed sensitivity to the essential oils. The essential oils of catnip, cinnamon, tea tree and thyme essential oils exhibited a large spectrum antimicrobial activities; those of clary sage and laurel inhibited the mycelial growth in a few fungal strains. The essential oils of cinnamon and thyme had the lowest MIC and MFC values against all the fungi assayed, followed by catnip, tea tree, clary sage and laurel. The use of these natural products rather than, the currently used antifungal chemicals, may be of interest given that: i) essential oils are of natural origin which means they are safer for human health and the environment and ii) there is less chance that the pathogenic microorganisms will develop resistance

The roles of news media as democratic fora, agenda setters, and strategic instruments in risk governance

This study analyzes news media’s role in governmental decision-making processes related to a gradually intensifying series of earthquakes resulting from gas drilling in the Netherlands, and catastrophic natural earthquakes in Italy. According to the risk governance actors interviewed in both cases, media play three roles, as: democratic fora, agenda setters, and strategic instruments. Media attention for risk can create ripple effects in governmental decision-making processes. However, media attention tends to be risk-event driven and focuses on direct newsworthy consequences of events. For ‘non-event risks’, or when newsworthiness after a risk-event fades, the media’s agenda setting and democratic fora roles are limited. This contributes to risk attenuation in society, potentially resulting in limited risk prevention and preparedness. Governmental actors report difficulties in using news media for strategic communication to facilitate risk governance because of media’s tendency towards sensationalism. Our research suggests that, in the governance of earthquake-risk news, media logic overrules other institutional logics only for a short while and not in the long run when the three roles of media do not reinforce each other

Phytochemical Analysis and Antiradical Properties of Sarcodon imbricatus (L.:Fr) Karsten:

The chemical composition of Sarcodon imbricatus (L.:Fr) Karsten (Hydnaceae) was evaluated to assess it as source of nutrients and nutraceuticals. The analyzed mushroom contains ergosterol and ergosterol peroxide. S. imbricatus methanolic extract showed a moderate antiradical activity (measured by DPPH radical scavenging activity). The combination of bioactive compounds and rich nutritional composition (high contents in protein, low content in fat, and its content of unsaturated fatty acids) makes the mushroom a good food

An Integrated Approach to the Evaluation of a Metabolomic Fingerprint for a Phytocomplex. Focus on Artichoke [Cynara cardunculus subsp. scolymus] Leaf:

The availability of reliable herbal formulations is essential in order to assure the maximal activity and to limit unwanted side-effects. The correct concentration of declared components of herbal products is a matter of health legislation and regulation, but is still a topic under debate in the field of quality control assessment. In the present work specific constituents of artichoke leaf extracts, considered as a test herbal product, were measured by standard spectrophotometric and HPLC methods (for quantitative determination of some components only), and results were correlated with the ESI-MS (showing the full metabolomic fingerprint). Phytocomplex stability over time was also investigated in batches submitted to different storage conditions. The results indicated excellent agreement between the two approaches in the measurement of total caffeoylquinic acids and chlorogenic acid contents, but the metabolomic ESI-MS method approach provides a more complete evaluation and monitoring of the composition of a herbal product, without focusing only on a single/few compound measurements. Therefore, the ESI-MS method can be proposed for the evaluation of the quality of complex matrices, such as those in a phytocomplex. Another aspect lies in the possibility to obtain a broad-spectrum stability control of herbal formulations, requiring minimal sample pre-processing procedures

In vitro testing of estragole in HepG2 cells: Cytokinesis-block micronucleus assay and cell-cycle analysis

The alkenylbenzene estragole (systematic name, 1-allyl-4-methoxybenzene) is a natural component of essential oils from various spices and herbs, including fennel, and it is used as a food and beverage flavouring agent. Estragole has been reported to be hepatocarcinogenic at high doses in rodents. However, in a previous in vitro study, we found that estragole did not exhibit cytotoxic effects after 4 hours of exposure, nor did it induce DNA damage or apoptosis in human HepG2 hepatoblastoma cells. As fennel tea is widely used for symptomatic treatment of spasmodic gastrointestinal conditions in infants, we aimed at further assessing its safety in a different experimental setting. We thus searched for possible cytogenetic effects and interference with cell-cycle progression in the same human hepatoblastoma cell line. Estragole did not show any clastogenic/aneugenic activities in the cytokinesis-block micronucleus assay, and no effects on cell-cycle checkpoints were observed

Anti-inflammatory and analgesic effects of Spartium junceum L. flower extracts: a preliminary study

Our objective was to study the anti-inflammatory and analgesic activity of extract of Spartium junceum L. flowers. Samples of flowers were collected from wild plants, dried, powdered, and extracted with hexane and methanol. The extracts were evaporated to dryness and then suspended in suitable solvent. They were then tested for anti-inflammatory activity in the carrageenin rat paw edema test and for analgesic activity in the Randall and Selitto mechanical pressure test and in the tail-flick test. Twenty-four hours after treatment, the gastric mucosa of each rat was observed macroscopically. Based on these results the hexane extract was fractioned by column chromatography, and the fractions obtained were tested in the same way. The results showed good anti-inflammatory activity only for a single fraction of the hexane extract, while all the extracts and all the other hexane fractions showed both peripheral and central analgesic activity. In rats treated with the tested compounds hyperemia and ulcers were absent. The data from this preliminary study reveal interesting pharmacological properties of S. junceum L. flowers extract related to the marked analgesic activity and the absence of gastric ulcerogenic activity

Analysis of Deterioration in the Crypt of the Abbey of Montecorona with Integrated Methods

A study of the problem of deterioration in the crypt of the Abbey of Montecorona (Perugia, Northen Umbria-Italy) is proposed, with the aim to achieve a knowledge of the indoor and subsoil conditions. First of all we studied the microbial biodiversity of the crypt, analyzing the presence of microorganisms by microscopic and cultivation methods. Then we investigated the influence of the environment upon colonisation and growth of those micro-organisms, monitoring the microclimate, especially the thermo-hygrometric conditions. Various Ground Penetrating Radar (GPR) System surveys were carried out in order to localise archaeological remains and wet buried structures involved in the deterioration process and, by means a velocity analysis, we evaluated the underground water content. Microclimatic, biological and geophysical results were compared, in order to eliminate the ambiguity inherent each method. The results permits us to identify properly the causes of the deterioration in the crypt