Angular analysis and branching fraction measurement of the decay B0→K*0μ+μ−

The angular distributions and the differential branching fraction of the decay B0→K*(892)0μ+μ− are studied using a data sample corresponding to an integrated luminosity of 5.2 fb−1collected with the CMS detector at the LHC in pp collisions at √s=7 TeV. From more than 400 signal decays, the forward–backward asymmetry of the muons, the K*(892)0 longitudinal polarization fraction, and the differential branching fraction are determined as a function of the square of the dimuon invariant mass. The measurements are in good agreement with standard model predictions

Measurement of jet multiplicity distributions in tt¯ production in pp collisions at s√=7TeV

This is the published version

The evaluation of arrangements for effective operation of the new Local Safeguarding Children Boards in England – final report

The evaluation of arrangements for effective operation of the new Local Safeguarding Children Boards in England – final repor

Increased sensitivity for detecting malaria parasites in human umbilical cord blood using scaled-up DNA preparation

BACKGROUND: All mothers donating umbilical cord blood units to the NHS cord blood bank undergo an assessment for the likelihood of prior exposure to malaria infection. Those deemed at risk due to a history of travel to, or residence in, malaria endemic regions are screened serologically to detect anti-malaria antibodies. A positive result excludes the use of the cord blood for transplant therapy unless a risk assessment can ensure that malaria transmission is extremely unlikely. This paper details the screening of cord blood units from malaria serology positive mothers to detect malaria parasite DNA using a highly sensitive nested PCR. METHODS: Uninfected blood from a healthy volunteer was spiked with known quantities of malaria parasites and 5 millilitre and 200 microlitre aliquots were subjected to DNA extraction using QIAamp DNA maxi and DNA mini kits respectively. Nested PCR, to detect malarial SSU rRNA sequences, was performed on the purified DNA samples to determine the limit of detection for this assay with both extraction methodologies. Following assay validation, 54 cord blood units donated by mothers who were positive for anti-malaria antibodies were screened by this approach. RESULTS: When DNA was purified from 5 millilitres of blood it was possible to routinely detect as few as 50 malaria parasites per millilitre using nested PCR. This equates to a significant increase in the sensitivity of the current gold standard nucleic acid amplification technique used to detect malaria parasites (routinely performed from > 200 microlitre volumes of blood). None of the 54 donated cord blood units from serology positive mothers tested positive for malaria parasites using this scaled up DNA preparation method. CONCLUSION: Serological testing for malaria parasites may be overly conservative, leading to unnecessary rejection of cord blood donations that lack malaria parasites and which are, therefore, safe for use in stem cell therapy