Otoacaríase psoróptica dos caprinos-Infestação subclínica

Naturally occuring subclinical psoroptic otoacariasis in goats is reported in Pernambuco and Rio de Janeiro State. Infestation rates of 59.3% and 46% were observed in goats for about twelve months of age in the States of Pernambuco and Rio de Janeiro, respectively.A otoacaríase psoróptica subclínica dos caprinos é assinalada nos Estados de Pernambuco e Rio de Janeiro. Índices de infestação de 59,3% e 46% foram observados em animais com idade aproximada de doze meses, criados no Estados de Pernambuco e Rio de Janeiro, respectivamente.

Use of a candidate gene array to delineate gene expression patterns in cattle selected for resistance or susceptibility to intestinal nematodes

In the present study, we use microarray technology to investigate the expression patterns of 381 genes with known association to host immune responses. Hybridization targets were derived from previously characterized bovine cDNAs. A total of 576 reporters (473 sequence-validated cDNAs and 77 controls) were spotted onto glass slides in two sets of four replicates. Two color, comparative hybridizations across both mesenteric lymph node (MLN) and small intestine mucosa (SIM) RNA samples were done between animals with previously demonstrated phenotypic differences based on natural exposure to gastro- intestinal (GI) nematodes over a 6-month exposure period. A total of 138 significant hybridization differences were detected by mixed model analysis of variance. A subset of these significant differences was validated by quantitative, real-time RT-PCR to assay transcript levels for 18 genes. These results confirmed that in the SIM, susceptible animals showed significantly higher levels in the genes encoding IGHG1, CD3E, ACTB, IRF1, CCL5 and C3, while in the MLN of resistant animals, higher levels of expression were confirmed for PTPRC, CD1D and ITGA4. Combined, the results indicate that immune responses against GI nematode infections involve multiple response pathways. Higher levels of expression for IgE receptor, integrins, complement, monocyte/macrophage and tissue factors are related to resistance. In contrast, higher levels of expression for immunoglobulin chains and TCRs are related to susceptibility. Identification of these genes provides a framework to better understand the genetic variation underlying parasite resistance