569 research outputs found
Fast and efficient generation of influenza A virus like particles from synthetic genes
Co-transfection of bacmids resulted in the generation of influenza A virus like particles in the supernatant of Sf9 cells. VLPs were purified by means of Sucrose gradient centrifugation and the expected results were confirmed by Electron microscopy, Western Blot analysis and hemagglutination assays
An Unusual Foreign Body in the Maxillary Antrum: A Case Report and Pertinent Review of Literature
Maxillary sinus is the largest of the paranasal sinuses and occupies the body of Maxilla. It is pyramidal in shape with base toward lateral wall of nose and apex directed laterally into thezygomatic process. It is roof is formed by the floor of the orbit and grooved by infraorbital nerve. Foreign bodies may present in the paranasal sinuses through a variety of traumatic andiatrogenic events. We describe a case where a sharp foreign body penetrated into the maxillary sinus. We used the open surgical approach, for the removal of sinus foreign body
Slow flows of an relativistic perfect fluid in a static gravitational field
Relativistic hydrodynamics of an isentropic fluid in a gravitational field is
considered as the particular example from the family of Lagrangian
hydrodynamic-type systems which possess an infinite set of integrals of motion
due to the symmetry of Lagrangian with respect to relabeling of fluid particle
labels. Flows with fixed topology of the vorticity are investigated in
quasi-static regime, when deviations of the space-time metric and the density
of fluid from the corresponding equilibrium configuration are negligibly small.
On the base of the variational principle for frozen-in vortex lines dynamics,
the equation of motion for a thin relativistic vortex filament is derived in
the local induction approximation.Comment: 4 pages, revtex, no figur
Impact of HuR inhibition by the small molecule MS-444 on colorectal cancer cell tumorigenesis.
Colorectal cancer (CRC) is the third most common cancer and a leading cause of cancer-related mortality. Observed during CRC tumorigenesis is loss of post-transcriptional regulation of tumor-promoting genes such as COX-2, TNFα and VEGF. Overexpression of the RNA-binding protein HuR (ELAVL1) occurs during colon tumorigenesis and is abnormally present within the cytoplasm, where it post-transcriptionally regulates genes through its interaction with 3\u27UTR AU-rich elements (AREs). Here, we examine the therapeutic potential of targeting HuR using MS-444, a small molecule HuR inhibitor. Treatment of CRC cells with MS-444 resulted in growth inhibition and increased apoptotic gene expression, while similar treatment doses in non-transformed intestinal cells had no appreciable effects. Mechanistically, MS-444 disrupted HuR cytoplasmic trafficking and released ARE-mRNAs for localization to P-bodies, but did not affect total HuR expression levels. This resulted in MS-444-mediated inhibition of COX-2 and other ARE-mRNA expression levels. Importantly, MS-444 was well tolerated and inhibited xenograft CRC tumor growth through enhanced apoptosis and decreased angiogenesis upon intraperitoneal administration. In vivo treatment of MS-444 inhibited HuR cytoplasmic localization and decreased COX-2 expression in tumors. These findings provide evidence that therapeutic strategies to target HuR in CRC warrant further investigation in an effort to move this approach to the clinic
The hodograph method applicability in the problem of long-scale nonlinear dynamics of a thin vortex filament near a flat boundary
Hamiltonian dynamics of a thin vortex filament in ideal incompressible fluid
near a flat fixed boundary is considered at the conditions that at any point of
the curve determining shape of the filament the angle between tangent vector
and the boundary plane is small, also the distance from a point on the curve to
the plane is small in comparison with the curvature radius. The dynamics is
shown to be effectively described by a nonlinear system of two
(1+1)-dimensional partial differential equations. The hodograph transformation
reduces that system to a single linear differential equation of the second
order with separable variables. Simple solutions of the linear equation are
investigated at real values of spectral parameter when the filament
projection on the boundary plane has shape of a two-branch spiral or a smoothed
angle, depending on the sign of .Comment: 9 pages, revtex4, 6 eps-figure
Finite time singularities in a class of hydrodynamic models
Models of inviscid incompressible fluid are considered, with the kinetic
energy (i.e., the Lagrangian functional) taking the form in 3D Fourier representation, where
is a constant, . Unlike the case (the usual Eulerian
hydrodynamics), a finite value of results in a finite energy for a
singular, frozen-in vortex filament. This property allows us to study the
dynamics of such filaments without the necessity of a regularization procedure
for short length scales. The linear analysis of small symmetrical deviations
from a stationary solution is performed for a pair of anti-parallel vortex
filaments and an analog of the Crow instability is found at small wave-numbers.
A local approximate Hamiltonian is obtained for the nonlinear long-scale
dynamics of this system. Self-similar solutions of the corresponding equations
are found analytically. They describe the formation of a finite time
singularity, with all length scales decreasing like ,
where is the singularity time.Comment: LaTeX, 17 pages, 3 eps figures. This version is close to the journal
pape
A survey of performance enhancement of transmission control protocol (TCP) in wireless ad hoc networks
This Article is provided by the Brunel Open Access Publishing Fund - Copyright @ 2011 Springer OpenTransmission control protocol (TCP), which provides reliable end-to-end data delivery, performs well in traditional wired network environments, while in wireless ad hoc networks, it does not perform well. Compared to wired networks, wireless ad hoc networks have some specific characteristics such as node mobility and a shared medium. Owing to these specific characteristics of wireless ad hoc networks, TCP faces particular problems with, for example, route failure, channel contention and high bit error rates. These factors are responsible for the performance degradation of TCP in wireless ad hoc networks. The research community has produced a wide range of proposals to improve the performance of TCP in wireless ad hoc networks. This article presents a survey of these proposals (approaches). A classification of TCP improvement proposals for wireless ad hoc networks is presented, which makes it easy to compare the proposals falling under the same category. Tables which summarize the approaches for quick overview are provided. Possible directions for further improvements in this area are suggested in the conclusions. The aim of the article is to enable the reader to quickly acquire an overview of the state of TCP in wireless ad hoc networks.This study is partly funded by Kohat University of Science & Technology (KUST),
Pakistan, and the Higher Education Commission, Pakistan
Hamiltonian magnetohydrodynamics: symmetric formulation, Casimir invariants, and equilibrium variational principles
The noncanonical Hamiltonian formulation of magnetohydrodynamics (MHD) is
used to construct variational principles for symmetric equilibrium
configurations of magnetized plasma including flow. In particular, helical
symmetry is considered and results on axial and translational symmetries are
retrieved as special cases of the helical configurations. The symmetry
condition, which allows the description in terms of a magnetic flux function,
is exploited to deduce a symmetric form of the noncanonical Poisson bracket of
MHD. Casimir invariants are then obtained directly from the Poisson bracket.
Equilibria are obtained from an energy-Casimir principle and reduced forms of
this variational principle are obtained by the elimination of algebraic
constraints.Comment: submitted to Physics of Plasmas, 16 page
Examining health-related quality of life in pediatric cancer patients with febrile neutropenia: Factors predicting poor recovery in children and their parents
Background
The impact febrile neutropenia (FN) has on the health-related quality of life (HRQoL) of children with cancer and their families is poorly understood. We sought to characterize the course of child and parent HRQoL during and following FN episodes.
Method
Data on HRQoL were collected in the multisite Australian Predicting Infectious ComplicatioNs in Children with Cancer (PICNICC) study. Participants were enrolled between November 2016 to January 2018. The Child Health Utility (CHU9D) was used to assess HRQoL in children (N = 167 FN events) and the Assessment of Quality of Life (AQoL-8D) was used to assess HRQoL parents (N = 218 FN events) at three time points: 0â3 days, 7-days and 30-days following the onset of FN. Group-based trajectory modeling (GBTM) was used to characterize the course of HRQoL.
Findings
For children, three distinct groups were identified: persistently low HRQoL over the 30-day course of follow-up (chronic: N = 78/167; 47%), increasing HRQoL after the onset of FN to 30 days follow-up (recovering: N = 36/167; 22%), and persistently high HRQoL at all three timepoints (resilient: N = 53/167; 32%). Applying these definitions, parents were classified into two distinct groups: chronic (N = 107/218, 49%) and resilient (N = 111/218, 51%). The child being male, having solid cancer, the presence of financial stress, and relationship difficulties between the parent and child were significant predictors of chronic group membership for both parents and children. Children classified as high-risk FN were significantly more likely to belong to the recovery group. Being female, having blood cancers and the absence of financial or relationship difficulties were predictive of both parents and children being in the resilient group.
Interpretation
Approximately half the children and parents had chronically low HRQoL scores, which did not improve following resolution of the FN episode. The child's sex, cancer type, and presence of financial and relationship stress were predictive of chronic group membership for both parents and children. These families may benefit from increased financial and psychosocial support during anti-cancer treatment
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