In vitro tolerance of Fusarium semitectum and Fusarium solani (anamorphic, Ascomycota) exposed to surface disinfectant commonly used in aquaculture

Se evaluó in vitro el efecto del: acido acético (AcH), ácido acético tamponado (AcH+AcNa), ioduro de potasio (IK) y Iodopovidona (IP) sobre la tasa de crecimiento (TC) de Fusarium solani (Mart.) Sacc. 1881 y Fusarium semitectum Berk. & Ravenel, 1875. Ambas especies fueron aisladas de ovas de Odontesthes bonariensis (Cuvier & Valenciennes, 1835). Las sustancias estudiadas son comúnmente utilizadas en acuicultura como desinfectantes de superficie. Las concentraciones ensayadas con actividad fungicida para ambas cepas fueron mucho más elevadas que las correspondientes concentraciones letales medias a 96 horas (CL50-96h) para ovas de pejerrey. Concentraciones inferiores a la CL50 - 96h exhibieron un efecto fungistático escaso o nulo.An in vitro evaluation of the antifungal effect of acetic acid (AcH), buffered acetic acid (AcH+AcNa), potassium iodide (KI) and Iodopovidone (IP); on the growth rate (GR) of Fusarium solani (Mart.) Sacc. 1881 and Fusarium semitectum Berk. & Ravenel, 1875 was performed. Both fungal species were isolated from eggs of the Argentinian silverside Odontesthes bonariensis (Cuvier & Valenciennes, 1835). The studied substances are commonly used in fish farming as surface disinfectants. The tested concentrations of this substances exhibited antifungal activity for both strains at concentrations higher than their 96h median lethal concentrations (LC50-96h) for the treated eggs; concentrations below the (LC50-96h) exhibited little or no effect.Fil: Pacheco Marino, Suani Giovanna. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cabello, Marta N.. Universidad Nacional de la Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Salibián, Alfredo. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina. Universidad Nacional de Luján; Argentin

In vitro tolerance of Fusarium semitectum and Fusarium solani (anamorphic, Ascomycota) exposed to surface disinfectant commonly used in aquaculture

An in vitro evaluation of the antifungal effect of acetic acid (AcH), buffered acetic acid (AcH+AcNa), potassium iodide (KI) and Iodopovidone (IP); on the growth rate (GR) of Fusarium solani (Mart.) Sacc. 1881 and Fusarium semitectum Berk. & Ravenel, 1875 was performed. Both fungal species were isolated from eggs of the Argentinian silverside Odontesthes bonariensis (Cuvier & Valenciennes, 1835). The studied substances are commonly used in fish farming as surface disinfectants. The tested concentrations of this substances exhibited antifungal activity for both strains at concentrations higher than their 96h median lethal concentrations (LC50-96h) for the treated eggs; concentrations below the (LC50-96h) exhibited little or no effect.Se evaluó in vitro el efecto del: acido acético (AcH), ácido acético tamponado (AcH+AcNa), ioduro de potasio (IK) y Iodopovidona (IP) sobre la tasa de crecimiento (TC) de Fusarium solani (Mart.) Sacc. 1881 y Fusarium semitectum Berk. & Ravenel, 1875. Ambas especies fueron aisladas de ovas de Odontesthes bonariensis (Cuvier & Valenciennes, 1835). Las sustancias estudiadas son comúnmente utilizadas en acuicultura como desinfectantes de superficie. Las concentraciones ensayadas con actividad fungicida para ambas cepas fueron mucho más elevadas que las correspondientes concentraciones letales medias a 96 horas (CL50-96h) para ovas de pejerrey. Concentraciones inferiores a la CL50 - 96h exhibieron un efecto fungistático escaso o nulo.Facultad de Ciencias Naturales y Muse

Compatibility of chemical insecticides and entomopathogenic fungi for control of soybean defoliating pest, Rachiplusia nu.

Fil: Pelizza, Sebastián Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios en Parasitología y Vectores; ArgentinaFil: Schalamuk, Santiago. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica; ArgentinaFil: Simón, María Rosa. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; ArgentinaFil: Stenglein, Sebastián Alberto. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Agronomía. Laboratorio de Biología Funcional y Biotecnología. Azul; ArgentinaFil: Pacheco Marino, Suani Giovanna. Instituto de Genética Veterinaria Ingeniero Fernando Noel Dulout. La Plata; ArgentinaFil: Scorsetti, Ana Clara. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Carlos Spegazzini; Argentin

Compatibility between entomopathogenic fungi and biorational insecticides in toxicity against <i>Ronderosia bergi</i> under laboratory conditions

Our aim was to evaluate the efficacy of combinations between two biorational insecticides (luphenuron, methoxyfenozide), a new synthetic chemical pesticide (rynaxypyr), and three entomopathogenic fungi strains (Beauveria bassiana LPSc 1067, LPSc1082), and Metarhizium anisopliae (LPSc 907) in the biocontrol of the pest grasshopper Ronderosia bergi (Sta°l) under laboratory conditions. The insecticides were tested at three concentrations: the average concentration recommended for application in the field (100 %) and 50 % and finally 25 % of that level. The fungal strains used were adjusted to 1 9 108, 1 9 106, and 1 9 104 conidia ml-1. The combinations of those insecticides with B. bassiana (LPSc 1067, LPSc 1082) and M. anisopliae (LPSc 907) caused higher mortality to R. bergi nymphs than any of the individual agents used alone. The three insecticides tested did not affect the isolates of the two species of entomopathogenic fungi employed. In conclusion, the use of these biorational insecticides in an IPM program aimed at control of the grasshopper R. bergi could be of value.Facultad de Ciencias Naturales y Muse

Compatibility between entomopathogenic fungi and biorational insecticides in toxicity against <i>Ronderosia bergi</i> under laboratory conditions

Our aim was to evaluate the efficacy of combinations between two biorational insecticides (luphenuron, methoxyfenozide), a new synthetic chemical pesticide (rynaxypyr), and three entomopathogenic fungi strains (Beauveria bassiana LPSc 1067, LPSc1082), and Metarhizium anisopliae (LPSc 907) in the biocontrol of the pest grasshopper Ronderosia bergi (Sta°l) under laboratory conditions. The insecticides were tested at three concentrations: the average concentration recommended for application in the field (100 %) and 50 % and finally 25 % of that level. The fungal strains used were adjusted to 1 9 108, 1 9 106, and 1 9 104 conidia ml-1. The combinations of those insecticides with B. bassiana (LPSc 1067, LPSc 1082) and M. anisopliae (LPSc 907) caused higher mortality to R. bergi nymphs than any of the individual agents used alone. The three insecticides tested did not affect the isolates of the two species of entomopathogenic fungi employed. In conclusion, the use of these biorational insecticides in an IPM program aimed at control of the grasshopper R. bergi could be of value.Facultad de Ciencias Naturales y Muse

Morphological techniques used in ichthyopathological diagnosis

In this review, different diagnostic techniques for identifying etiology of fish disease are described. Among microscopic techniques, we find rapid diagnostic tests to observe parasites, gill diseases and bacteria; routine histopathological techniques are also used. In samples having bone tissue, decalcification methods are employed. In recent years, apart from using immunohistochemistry and electron microscopy, morphological techniques allow a deep study of disease pathogenesis. At present, molecular techniques for pathogen identification and atomic-absorption spectrophotometry to measure the concentration and distribution of toxic metals in tissues they also use. In conclusion, different techniques can contribute to the evaluation of disorders and provide tools for a better understanding in fish medicine.Facultad de Ciencias Veterinaria

Morphological techniques used in ichthyopathological diagnosis

In this review, different diagnostic techniques for identifying etiology of fish disease are described. Among microscopic techniques, we find rapid diagnostic tests to observe parasites, gill diseases and bacteria; routine histopathological techniques are also used. In samples having bone tissue, decalcification methods are employed. In recent years, apart from using immunohistochemistry and electron microscopy, morphological techniques allow a deep study of disease pathogenesis. At present, molecular techniques for pathogen identification and atomic-absorption spectrophotometry to measure the concentration and distribution of toxic metals in tissues they also use. In conclusion, different techniques can contribute to the evaluation of disorders and provide tools for a better understanding in fish medicine.Facultad de Ciencias Veterinaria

Distribución del parásito <i>Lernaea cyprinacea</i> en el territorio argentino

Lernaea cyprinacea fue introducida en América, a principios del siglo XX, junto con peces tropicales de cultivo y/u ornamentales. Los efectos deletéreos sobre los hospedadores se identifican de forma sinèrgica, los propios de la actividad parasitaria y los causados por la colonización de agentes oportunistas. El presente trabajo pretende divulgar los hallazgos de L. cyprinacea en diferentes ambientes de Argentina.Facultad de Ciencias VeterinariasFacultad de Ciencias Naturales y Muse

Compatibility between entomopathogenic fungi and biorational insecticides in toxicity against <i>Ronderosia bergi</i> under laboratory conditions

Our aim was to evaluate the efficacy of combinations between two biorational insecticides (luphenuron, methoxyfenozide), a new synthetic chemical pesticide (rynaxypyr), and three entomopathogenic fungi strains (Beauveria bassiana LPSc 1067, LPSc1082), and Metarhizium anisopliae (LPSc 907) in the biocontrol of the pest grasshopper Ronderosia bergi (Sta°l) under laboratory conditions. The insecticides were tested at three concentrations: the average concentration recommended for application in the field (100 %) and 50 % and finally 25 % of that level. The fungal strains used were adjusted to 1 9 108, 1 9 106, and 1 9 104 conidia ml-1. The combinations of those insecticides with B. bassiana (LPSc 1067, LPSc 1082) and M. anisopliae (LPSc 907) caused higher mortality to R. bergi nymphs than any of the individual agents used alone. The three insecticides tested did not affect the isolates of the two species of entomopathogenic fungi employed. In conclusion, the use of these biorational insecticides in an IPM program aimed at control of the grasshopper R. bergi could be of value.Facultad de Ciencias Naturales y Muse

Morphological techniques used in ichthyopathological diagnosis

In this review, different diagnostic techniques for identifying etiology of fish disease are described. Among microscopic techniques, we find rapid diagnostic tests to observe parasites, gill diseases and bacteria; routine histopathological techniques are also used. In samples having bone tissue, decalcification methods are employed. In recent years, apart from using immunohistochemistry and electron microscopy, morphological techniques allow a deep study of disease pathogenesis. At present, molecular techniques for pathogen identification and atomic-absorption spectrophotometry to measure the concentration and distribution of toxic metals in tissues they also use. In conclusion, different techniques can contribute to the evaluation of disorders and provide tools for a better understanding in fish medicine.Facultad de Ciencias Veterinaria