Resistencia antibiótica en Klebsiella pneumoniae y Acinetobacter baumanni

En estos últimos años, bacterias saprófitas del tracto intestinal como la Klebsiella pneumoniae, uno de los principales patógenos de las infecciones nosocomiales, han adquirido tales resistencias enzimáticas a través de plásmidos que son muy limitados los tratamientos antimicrobianos que existen actualmente para combatirlas. A la aparición de las betalactamasas de espectro extendido durante los 90, en el año 2011 se ha sumado y masificado la serin carbapenemasa denominada KPC, que ya es considerada una endemia en nuestro país y que ha modificado sustancialmente el concepto de resistencia antimicrobiana, recurriendo a terapias combinadas de antibióticos tanto sensibles como resistentes.Facultad de Ciencias Veterinaria

Resistencia antibiótica en Klebsiella pneumoniae y Acinetobacter baumanni

En estos últimos años, bacterias saprófitas del tracto intestinal como la Klebsiella pneumoniae, uno de los principales patógenos de las infecciones nosocomiales, han adquirido tales resistencias enzimáticas a través de plásmidos que son muy limitados los tratamientos antimicrobianos que existen actualmente para combatirlas. A la aparición de las betalactamasas de espectro extendido durante los 90, en el año 2011 se ha sumado y masificado la serin carbapenemasa denominada KPC, que ya es considerada una endemia en nuestro país y que ha modificado sustancialmente el concepto de resistencia antimicrobiana, recurriendo a terapias combinadas de antibióticos tanto sensibles como resistentes.Facultad de Ciencias Veterinaria

Acute Toxicity of Opuntia Ficus Indica and Pistacia Lentiscus Seed Oils in Mice

Opuntia ficus indica and Pistacia lentiscus L. seeds are used in traditional medicine. The objective of this study was to investigate the toxicity of the fixed oil of Opuntia ficus indica and Pistacia lentiscus L. seeds in mice through determination of LD50 values, and also the physicochemical characteristics of the fixed oil of these oils. The acute toxicity of their fixed oil were also investigated in mice using the method of Kabba and Berhens. The fixed oil of Pistacia lentiscus and Opuntia ficus indica seeds were extracted and analyzed for its chemical and physical properties such as acid value, free fatty acid percentage (% FFA), iodine index, and saponification value as well as refractive index and density. LD50 values obtained by single doses, orally and intraperitoneally administered in mice, were respectively 43 ± 0,8 ;[40.7- 45.4 ] ml/kg body wt. p.o. and 2.72 ± 0,1 ;[2.52–2.92] ml/kg body wt. i.p. for Opuntia ficus indica; and 37 ± 1 ;[34.4 – 39.8 ] ml/kg body wt. p.o. and 2.52 ± 0,2 ;[2.22 – 2.81 ] ml/kg body wt. i.p. for Pistacia lentiscus respectively. The yields of seed oil were respectively calculated as 20.25% and 10.41%. The acid and free fatty acid values indicated that the oil has a low acidity

A comparison of children's experiences with fixed and removable functional appliances: A qualitative study.

INTRODUCTION: The objective of this study was to compare children's experiences and perceptions of treatment with Hanks-Herbst (HH) and modified Twin-block (MTB) functional appliances. METHODS: A pragmatic nested qualitative study was undertaken in a single hospital setting. Participants from a randomized controlled trial (International Standard Randomised Controlled Trial Number 11717011) wearing HH and/or MTB appliances were interviewed using a topic guide in a one-to-one, semistructured format. Interviews were recorded and transcribed verbatim for framework methodology analysis until data saturation was reached. RESULTS: Eighteen participants (HH, 7: MTB, 4; switched group, 7) were interviewed. Thirteen codes were constructed and grouped into 3 themes: (1) functional impairment and symptoms, (2) psychosocial factors and impacts, and (3) feedback on appliances and patient care. Both appliances had a negative impact on quality of life, with disruption to children's daily routines and psychological well-being. Speaking was more problematic for MTB participants, whereas HH participants encountered mastication and breakage issues. HH was preferred by most participants, as its nonremovable feature meant less managing and self-discipline was required. MTB was considered a suitable option for children with good self-discipline and who preferred a versatile lifestyle. Feedback included wishes for the availability of multiple appliance options and a degree of autonomy in decision-making processes. CONCLUSIONS: HH and MTB can negatively affect children's quality of life. Participants preferred HH over MTB because of its nonremovable feature, and children requested to be empowered during decision-making processes

USE OF CHROMAGAR ORIENTATION FOR PRESEMPTIVE IDENTIFICATION OF ENTEROCOCCI AND CHARACTERIZATION OF ANTIMICROBIAL RESISTANCE OF THE ISOLATES

This study was conducted to evaluate the use of CHROMagar Orientation for presumptive identification of enterococci from poultry, and to characterize the antimicrobial resistance of the isolates. Strains identification with conventional methods allowed confirmation of Enterococcus genus membership of all of the isolates on CHROMagar orientation (100%). Of the 141 enterococci isolates obtained from chicken, 81 (57.45%) were identified as Enterococcus faecalis and 60 (42.55%) as Enterococcus faecium. The antimicrobial susceptibility test presented high level of resistance to Tetracycline (89%) and Erythromycin (65%), low level of resistance to High Level of Streptomycin, Penicillin and Ciprofloxacin (13%, 10% and 9% respectively). Few of isolates were resistant to Chloramphenicol (2%). All the strains were susceptible to High Level of Gentamycin, Ampicillin, Vancomycin and Nitrofurantoin. The predominant phenotype of resistance pattern identified in both E.faecalis and E.faecium was (Erythromycin -Tetracycline)

EVALUATION OF SEMEN QUALITY IN RABBIT OF LOCAL ALGERIAN POPULATION AND SYNTHETIC LINE IN THE SUMMER SEASON: A COMPARATIVE STUDY

The aim of our study was to compare the libido and semen characteristics in 24 rabbits of local and synthetic line during summer season. Results showed that both breeds had similar (p ˃0.05) libido (13.92 vs 16.85 s). Gel free volume (0.88 vs. 0.87 mL), pH (7.51 vs. 7.65), and live sperm (56.21 vs. 55.88%) were similar. Local population had higher semen concentration (398.50 x106 /mL vs. 328.90 x106 /mL) and percentage of abnormal spermatozoa (36.54 vs 30.28%). Massal and individual motility (p=0.006 and p=0.008) were significantly increased in local population. Kinetic traits for Local population were significantly greater (P<0.05), except for VCL, ALH and BCF. We conclude that, rabbit bucks of local population had a good ability of adaptation to produce in a hot climate