This study was conducted to evaluate the use of CHROMagar Orientation for presumptive identification of enterococci from poultry, and to characterize the antimicrobial resistance of the isolates. Strains identification with conventional methods allowed confirmation of Enterococcus genus membership of all of the isolates on CHROMagar orientation (100%). Of the 141 enterococci isolates obtained from chicken, 81 (57.45%) were identified as Enterococcus faecalis and 60 (42.55%) as Enterococcus faecium. The antimicrobial susceptibility test presented high level of resistance to Tetracycline (89%) and Erythromycin (65%), low level of resistance to High Level of Streptomycin, Penicillin and Ciprofloxacin (13%, 10% and 9% respectively). Few of isolates were resistant to Chloramphenicol (2%). All the strains were susceptible to High Level of Gentamycin, Ampicillin, Vancomycin and Nitrofurantoin. The predominant phenotype of resistance pattern identified in both E.faecalis and E.faecium was (Erythromycin -Tetracycline)
