Embryonic POU5F1 is Required for Expanded Bovine Blastocyst Formation.

POU5F1 is a transcription factor and master regulator of cell pluripotency with indispensable roles in early embryo development and cell lineage specification. The role of embryonic POU5F1 in blastocyst formation and cell lineage specification differs between mammalian species but remains completely unknown in cattle. The CRISPR/Cas9 system was utilized for targeted disruption of the POU5F1 gene by direct injection into zygotes. Disruption of the bovine POU5F1 locus prevented blastocyst formation and was associated with embryonic arrest at the morula stage. POU5F1 knockout morulas developed at a similar rate as control embryos and presented a similar number of blastomeres by day 5 of development. Initiation of SOX2 expression by day 5 of development was not affected by lack of POU5F1. On the other hand, CDX2 expression was aberrant in embryos lacking POU5F1. Notably, the phenotype observed in bovine POU5F1 knockout embryos reveals conserved functions associated with loss of human embryonic POU5F1 that differ from Pou5f1- null mice. The similarity observed in transcriptional regulation of early embryo development between cattle and humans combined with highly efficient gene editing techniques make the bovine a valuable model for human embryo biology with expanded applications in agriculture and assisted reproductive technologies

Effects on soybean growth and yield of wheat-soybean intercropping system

It has been demonstrated that soybean (Glycine max) produces lower yields at relay intercropping with wheat (Triticum aestivum) than if it is sown as a sole crop. However, most studies considered wider or irregular soybean row spacing, compromising its capacity to recover after wheat harvest. This work studied the stress effects in relay soybean intercropping and suggests narrowing row spacing to improve soybean performance. The aims were (i) to compare growth and yield of two planting patterns and (ii) to separate the effect of water stress (WS) from the effects of other stress factors (OSF) induced by wheat on intercropping soybean. WS was evaluated comparing above-ground dry and grain yield of irrigated and non irrigated intercropping soybean, and OSF was evaluated comparing intercropping soybean with another treatment in which wheat straw (aerial biomass) was eliminated at soybean emergence, both irrigated treatments. In wheat, similar yields were obtained in treatments with an intercropping planting pattern with two rows for wheat and one for soybean (2:1) compared to three rows for wheat and one row for soybean (3:1). However, intercropping soybean at narrow row spacing (52 cm; 2:1) improve yielded 23% more than intercropping at 70 cm (3:1). During wheat-soybean coexistence, OSF prevailed on soybean and this effect persisted in later stages. After wheat harvest, OSF reduced the amount of light interception from R1 to R5 and depressed the crop growth rate (CGR) in 34%. However, in this period, WS also affected the radiation use efficiencies (RUE) which explained the greater fraction (66%) of the total stress induced by wheat in soybean CGR. Intercrop soybean yielded 182 g m-2 less compared to the unstressed sole crop control. Considering the wheat effects on soybean growth, 63% (116.5 g m-2) of the total yield lost were due to WS. Therefore, most of the performance of relay intercropping soybean was linked with water disponibility since early stages. However, at optimum water condition wheat competition by light and resources also affected soybean yield (OSF: 37%).EEA BarrowFil: Ross, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Barrow; ArgentinaFil: Abbate, Pablo Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentin

Hydrologic Restoration of the Biscayne Bay Coastal Wetlands: mosquito and drainage ditch inventory and recommendations

The management and restoration of the Biscayne Bay Coastal Wetlands (BBCW) is a complex issue. Unlike other natural areas under the supervision of the National Park System, the BBCW had endured many years of neglect and abuse by homesteaders who, prior to the establishment of Biscayne National Monument in 1968, had free reign of the area and tried to farm and develop the land by ditching and infilling. Furthermore, public works projects, dating back to the early 1900’s for mosquito control, land reclamation, and storm surge protection along with homesteader activities have combined to compartmentalize the coastal wetlands of present Biscayne National Park and adjacent marshes

Building a Global Process Standard at the Most International Company on Earth: DHL Express

As “the most international company on earth,” DHL Express promised to deliver packages between almost any pair of countries within a defined time-frame. To fulfill this promise, the company had introduced a set of global business and technology standards. While standardization had many advantages (improving service for multinational customers, faster response to changes in import/export regulations, sharing of best practices, etc.), it created impediments to local innovation and responsiveness in DHL Express’ network of 220 countries/territories. Reconciling standardization-innovation tradeoffs is a critical management issue for global companies in the digital economy. This case study describes one large, successful company’s approach to the tradeoff of standardization versus innovation

Hypercolumns for Object Segmentation and Fine-grained Localization

Recognition algorithms based on convolutional networks (CNNs) typically use the output of the last layer as feature representation. However, the information in this layer may be too coarse to allow precise localization. On the contrary, earlier layers may be precise in localization but will not capture semantics. To get the best of both worlds, we define the hypercolumn at a pixel as the vector of activations of all CNN units above that pixel. Using hypercolumns as pixel descriptors, we show results on three fine-grained localization tasks: simultaneous detection and segmentation[22], where we improve state-of-the-art from 49.7[22] mean AP^r to 60.0, keypoint localization, where we get a 3.3 point boost over[20] and part labeling, where we show a 6.6 point gain over a strong baseline.Comment: CVPR Camera read

Landscape Pattern – Marl Prairie/Slough Gradient: Vegetation Composition along the Gradient and Decadal Vegetation Change Pattern in Shark Slough: Annual Report 2012

In the southern Everglades, vegetation in both the marl prairie and ridge and slough landscapes is sensitive to large-scale restoration activities associated with the Comprehensive Everglades Restoration Plan (CERP) authorized by the Water Resources Development Act (WRDA) 2000 to restore the south Florida ecosystem. More specifically, changes in hydrologic regimes at both local and landscape scales are likely to affect vegetation composition along marl prairie-slough gradient resulting in a shift in boundary between plant communities in these landscapes. To strengthen our ability to assess how vegetation would respond to changes in underlying ecosystem drivers along the gradient, an improved understanding of reference conditions of plant community structure and function, and their responses to major stressors is important. In this regard, a study of vegetation structure and composition in relation to physical and biological processes along the marl prairie-slough gradient was initiated in 2005, and has continued through 2012 with funding from US Army Corps of Engineers (USACOE) (Cooperative Agreement # W912HZ-09-2-0018 Modification No.: P00002). This study addresses the hypothesis with respect to RECOVER-MAP monitoring item 3.1.3.5 – “Marl Prairie/Slough Gradients; patterns and trends in Shark Slough marshes and associated marl prairies”

The 2008 Terrestrial Vegetation of Biscayne National Park FL, USA Derived From Aerial Photography, NDVI, and LiDAR

Established as a National Park in 1980, Biscayne National Park (BISC) comprises an area of nearly 700 km2 , of which most is under water. The terrestrial portions of BISC include a coastal strip on the south Florida mainland and a set of Key Largo limestone barrier islands which parallel the mainland several kilometers offshore and define the eastern rim of Biscayne Bay. The upland vegetation component of BISC is embedded within an extensive coastal wetland network, including an archipelago of 42 mangrove-dominated islands with extensive areas of tropical hardwood forests or hammocks. Several databases and vegetation maps describe these terrestrial communities. However, these sources are, for the most part, outdated, incomplete, incompatible, or/and inaccurate. For example, the current, Welch et al. (1999), vegetation map of BISC is nearly 10 years old and represents the conditions of Biscayne National Park shortly after Hurricane Andrew (August 24, 1992). As a result, a new terrestrial vegetation map was commissioned by The National Park Service Inventory and Monitoring Program South Florida / Caribbean Network