Bibliography of Structuralism III

In two occasions a Bibliography of Structuralism has been published in Erkenntnis (1989, 1994). Since then a lot of water has flowed under the bridge and the structuralist program has shown a continuous development. The aim of the present bibliography is to celebrate the 25th anniversary of the publication of An Architectonic for Science –structuralism’s main reference work– and of its recent translation into Spanish by updating the previous bibliographies with titles which have appeared since 1994 as well as before that year but which are not included in them. As in the former deliveries, this bibliography only covers books and articles that are concerned directly with the structuralist approach in the philosophy of science. We would like to thank the many colleagues who have helped us in collecting all the information. Notwithstanding we apologize in advance for the possible entries that we missed to include in this third Bibliography of Structuralism

Art and devotion around death. The brotherhood of souls of Los Realejos

Las cofradías de ánimas de las dos principales parroquias de Los Realejos han marcado el devenir de sus templos. Sus imponentes cuadros de ánimas, retablos e imágenes asociadas a ellos han marcado la vida parroquial durante siglos. La fundación de ambas confraternidades, a partir de una idea después de la muerte y del culto a las almas que se encontraban en el purgatorio para poder alcanzar la gloria, es el origen de estos cultos. Además, su crecimiento patrimonial, en el caso del Realejo Alto mayor que en Realejo Bajo, acabaría por dotar a ambas parroquias de una importante fuente patrimonial hasta su desaparición durante los siglos xix y xx, quedando los vestigios que hoy conservamos en la parroquia de Santiago y que el fuego arrasó en el caso de la parroquia de la Concepción.The brotherhoods of souls of parishes of Los Realejos they have marked the history of their temples. His imposing paintings of souls, altarpieces, and sculptures have marked the parish life for centuries. The fundation of both brotherghoods, from a idea after death and the cult of souls of Purgatory to be able to achieve glory is the origin of these cults. In addition, its patrimonial growth, in the case of Realejo Alto is greater than Realejo Bajo, it would end up giving both parishes until their disappearance during the century 19th and 20th, remaining the vestiges that today we conserve in de parish of Santiago and that the fire burned in the parish of the Conception

For the sufrague and relief of deceased. Sibling priests saint peter’s brotherhood in La Laguna and it’s reflex in Tenerife

Las cofradías de sacerdotes son un eslabón más dentro de las instituciones gremiales que surgieron durante el Antiguo Régimen. A raíz de la fundación de la primera confraternidad en la parroquia de Nuestra Señora de la Concepción de La Laguna, fueron naciendo más por los distintos templos, en especial los del norte de la isla de Tenerife. Las normas que las regían, los cultos del Martes Santo en honor de las Lágrimas de San Pedro y el patrimonio que desarrollaron son los principales ejes de este artículo.The priest’s brotherhood are a link more inside the union institutions which appears during the Old Regimen (es nombre propio, también se puede poner en español pero me suena mejor en inglés en línea con el texto). Since the foundation of the first fellowship in Nuestra Señora de la Concepción in La Laguna’s chunch were born more ones by the different churches, in special in the north of Tenerife. The rules which followed them, Saint Tuesday’s workship in honor of Saint Peter’s tears and the heritage which developed are the main points of Thais article

Expression of Fluorescent Genes in Trypanosoma cruzi and Trypanosoma rangeli (Kinetoplastida: Trypanosomatidae): Its Application to Parasite-Vector Biology

Two Trypanosoma cruzi-derived cloning vectors, pTREX-n and pBs:CalB1/CUB01, were used to drive the expression of green fluorescent protein (GFP) and DsRed in Trypanosoma rangeli Tejera, 1920, and Trypanosoma cruzi Chagas, 1909, isolates, respectively. Regardless of the species, group, or strain, parasites harboring the transfected constructs as either episomes or stable chromosomal integrations showed high-level expression of fluorescent proteins. Tagged flagellates of both species were used to experimentally infect Rhodnius prolixus Stal, 1953. In infected bugs, single or mixed infections of T. cruzi and T. rangeli displayed the typical cycle of each species, with no apparent interspecies interactions. In addition, infection of kidney monkey cells (LLC-MK2) with GFP-T. cruzi showed that the parasite retained its fluorescent tag while carrying out its life cycle within cultured cells. The use of GFP-tagged parasites as a tool for biological studies in experimental hosts is discussed, as is the application of this method for copopulation studies of same-host parasitesFil: Guevara, Palmira. Universidad Central de Venezuela; VenezuelaFil: Dias, Manuel. Universidad de los Andes; ColombiaFil: Rojas, Agustina. Universidad de los Andes; ColombiaFil: Crisante, Gladys. Universidad de los Andes; ColombiaFil: Abreu Blanco, Maria Teresa. Universidad Central de Venezuela; VenezuelaFil: Umezawa, Eufrozina. Universidade de Sao Paulo; BrasilFil: Vazquez, Martin Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Levin, Mariano Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Añez, Nestor. Universidad de los Andes; ColombiaFil: Ramirez, Jose Luis. Instituto de Estudios Avanzados; Venezuel

Brazil: The Difficult Path Toward Democratization of Civil Law

The Federal Constitution of 1988 reinstated the democratic rule of law in Brazil. Based on fundamental rights, the “Citizen’s Constitution” shaped the model of a welfare State, which objectives consist in the construction of a free, just and solidary society, the eradication of poverty and substandard living conditions and the promotion of the well-being of all, without prejudice as to origin, race, sex, color, age and any other forms of discrimination. From this context, the constitutional idealization of family democracy emerges, specifically represented in this paper by the recognition of civil union be-tween people of the same sex as family units. The democratization of civil law in matters of family law, beyond the interpretation of the legal norms in force according to the Constitution, demands the understanding of what constitutes each individual (or group) that is recipient of the principles of affection and solidarity. In the historic 2011 ruling, the Federal Supreme Court equated same-sex unions and opposite-sex unions as to rights and duties. However, the Court failed to advance in the unavoidable debate on gender issues

Air pollution and heart failure: relationship with the ejection fraction

To study whether the concentrations of particulate matter in ambient air are associated with hospital admission due to heart failure in patients with heart failure with preserved ejection fraction and reduced ejection fraction

Multi-compartment and multi-host vector suite for recombinant protein expression and purification

Recombinant protein expression has become an invaluable tool in basic and applied research. The accumulated knowledge in this field allowed the expression of thousands of protein targets in a soluble, pure, and homogeneous state, essential for biochemical and structural analyses. A lot of progress has been achieved in the last decades, where challenging proteins were expressed in a soluble manner after evaluating different parameters such as host, strain, and fusion partner or promoter strength, among others. In this regard, we have previously developed a vector suite that allows the evaluation of different promoters and solubility enhancer-proteins, through an easy and efficient cloning strategy. Nonetheless, the proper expression of many targets remains elusive, requiring, for example, the addition of complex post-translation modifications and/or passage through specialized compartments. In order to overcome the limitations found when working with a single subcellular localization and a single host type, we herein expanded our previously developed vector suite to include the evaluation of recombinant protein expression in different cell compartments and cell hosts. In addition, these vectors also allow the assessment of alternative purification strategies for the improvement of target protein yields

Multi-Compartment and Multi-Host Vector Suite for Recombinant Protein Expression and Purification

Recombinant protein expression has become an invaluable tool in basic and applied research. The accumulated knowledge in this field allowed the expression of thousands of protein targets in a soluble, pure, and homogeneous state, essential for biochemical and structural analyses. A lot of progress has been achieved in the last decades, where challenging proteins were expressed in a soluble manner after evaluating different parameters such as host, strain, and fusion partner or promoter strength, among others. In this regard, we have previously developed a vector suite that allows the evaluation of different promoters and solubility enhancer-proteins, through an easy and efficient cloning strategy. Nonetheless, the proper expression of many targets remains elusive, requiring, for example, the addition of complex post-translation modifications and/or passage through specialized compartments. In order to overcome the limitations found when working with a single subcellular localization and a single host type, we herein expanded our previously developed vector suite to include the evaluation of recombinant protein expression in different cell compartments and cell hosts. In addition, these vectors also allow the assessment of alternative purification strategies for the improvement of target protein yields

Multiscale analysis of the randomization limits of the chromosomal gene organization between Lepidoptera and Diptera

How chromosome gene organization and gene content evolve among distantly related and structurally malleable genomes remains unresolved. This is particularly the case when considering different insect orders. We have compared the highly contiguous genome assemblies of the lepidopteran Danaus plexippus and the dipteran Drosophila melanogaster, which shared a common ancestor around 290 Ma. The gene content of 23 out of 30 D. plexippus chromosomes was significantly associated with one or two of the six chromosomal elements of the Drosophila genome, denoting common ancestry. Despite the phylogenetic distance, 9.6% of the 1-to-1 orthologues still reside within the same ancestral genome neighbourhood. Furthermore, the comparison D. plexippus–Bombyx mori indicated that the rates of chromosome repatterning are lower in Lepidoptera than in Diptera, although still within the same order of magnitude. Concordantly, 14 developmental gene clusters showed a higher tendency to retain full or partial clustering in D. plexippus, further supporting that the physical association between the SuperHox and NK clusters existed in the ancestral bilaterian. Our results illuminate the scope and limits of the evolution of the gene organization and content of the ancestral chromosomes to the Lepidoptera and Diptera while helping reconstruct portions of the genome in their most recent common ancestor