82 research outputs found
Identification of a Strain of Peanut Chlorotic Streak Virus Causing Chlorotic Vein Banding Disease of Groundnut in India
A virus disease characterized by chlorotic vein banding, chlorotic line pattern along the margins or midrib of mature leaflets and chlorotic spots/rings was observed on commercial groundnut crops in Rayalaseema area of Andhra Pradesh with an incidence from 1% to nearly 60%. The virus was transmitted by mechanical inoculation in extracts prepared with 0.01 M potassium phosphate butter, pH 8.0 to 21 species from the Chenopodiaceae, Cruciferae, Leguminosae and Solanaceae, Chenopodium quinoa was found to be a good local lesion host. The virus was neither seed-transmitted through 1591 groundnut seeds nor aphid-transmitted by Aphis craccivora, Myzus persicae and Rhopalosiphum maidis either in non-persistent or semi-persistent manner. The virus remained infective in buffered tobacco leaf sap at a dilution of 10â5; in a 10â1 dilution of buffered sap the virus was infective for 2â3 days at 22â29°C or when heated to 65°C for 10 min but not to 70°C.
Clarification treatments with organic solvents with 10% chloroform was least damaging. The virus was purified from Nicotiana rustica leaves. Purified virus contained isometric particles of 51 nm in diameter with an electron dense core of 22 nm and two major polypeptides of 76 kDa and 36 kDa. A polyclonal antiserum to this virus was produced. In agar gel double diffusion, enzyme-linked immunosorbent assay and in electro-blot immunoassay rests the virus was related to peanut chlorotic streak virus and not to cauliflower mosaic, figwort mosaic and soybean chlorotic mottle viruse
Yield of varieties of Cucurbita pepo preimmunized with mild strains of Papaya ringspot virus - type W and Zucchini yellow mosaic virus
The Prehistory of Potyviruses: Their Initial Radiation Was during the Dawn of Agriculture
Background: Potyviruses are found world wide, are spread by probing aphids and cause considerable crop damage. Potyvirus is one of the two largest plant virus genera and contains about 15% of all named plant virus species. When and why did the potyviruses become so numerous? Here we answer the first question and discuss the other. Methods and Findings: We have inferred the phylogenies of the partial coat protein gene sequences of about 50 potyviruses, and studied in detail the phylogenies of some using various methods and evolutionary models. Their phylogenies have been calibrated using historical isolation and outbreak events: the plum pox virus epidemic which swept through Europe in the 20th century, incursions of potyviruses into Australia after agriculture was established by European colonists, the likely transport of cowpea aphid-borne mosaic virus in cowpea seed from Africa to the Americas with the 16th century slave trade and the similar transport of papaya ringspot virus from India to the Americas. Conclusions/Significance: Our studies indicate that the partial coat protein genes of potyviruses have an evolutionary rate of about 1.1561024 nucleotide substitutions/site/year, and the initial radiation of the potyviruses occurred only about 6,600 years ago, and hence coincided with the dawn of agriculture. We discuss the ways in which agriculture may have triggered the prehistoric emergence of potyviruses and fostered their speciation
Abstracts of presentations on plant protection issues at the xth international congress of virology: August 11-16,1996 Binyanei haOoma, Jerusalem, Israel Part 2 Plenary Lectures
Biomass, virus concentration, and symptomatology of cucurbits infected by mild and severe strains of Papaya ringspot virus
Comparison of two strains of peanut stripe virus in Taiwan.
Two strs (Ts and Tc) of peanut stripe potyvirus (PStV) naturally occurring in groundnut in Taiwan were isolated. PStV-Ts induced severe mosaic and systemic necrotic symptoms differing from the stripe symptoms induced by PStV-Tc and previously described PStV isolates. The biological properties of PStV-Ts were very similar to those of PStV-Tc and other known PStV isolates. Seed transmission rates of PStV-Ts in groundnut averaged 12.5%. The purified capsid and cylindrical inclusion proteins of PStV-Ts consisted of single species of protein monomer with MW of 36â000 and 71â000, respectively. In serological tests, PstV-Ts was indistinguishable from PStV-Tc and 2 PStV isolates from the USA but different from 14 other potyviruses
Analysis of in vitro Translation of Bean Yellow Mosaic Virus RNA: Inhibition of Proteolytic Processing by Antiserum to the 49K Nuclear Inclusion Protein
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