Fast Ground State Manipulation of Neutral Atoms in Microscopic Optical Traps

We demonstrate Rabi flopping at MHz rates between ground hyperfine states of neutral $^{87}$Rb atoms that are trapped in two micron sized optical traps. Using tightly focused laser beams we demonstrate high fidelity, site specific Rabi rotations with crosstalk on neighboring sites separated by $8 \mu\rm m$ at the level of $10^{-3}$. Ramsey spectroscopy is used to measure a dephasing time of $870 \mu\rm s$ which is $\approx$ 5000 times longer than the time for a $\pi/2$ pulse.Comment: 4 pages, 4 figure

Refractive Index Enhancement with Vanishing Absorption in an Atomic Vapor

We report a proof-of-principle experiment where the refractive index of an atomic vapor is enhanced while maintaining vanishing absorption of the beam. The key idea is to drive alkali atoms in a vapor with appropriate control lasers and induce a gain resonance and an absorption resonance for a probe beam in a two-photon Raman configuration. The strength and the position of these two resonances can be manipulated by changing the parameters of the control lasers. By using the interference between these two resonances, we obtain an enhanced refractive index without an increase in the absorption.Comment: 11 pages, 4 figure

Análise da expressão diferencial de genes de raízes de Arachis stenosperma Krapov. & W.C. Gregory infectadas com o fitonematóide Meloidogyne arenaria (Neal) Chitwood raça 1.

bitstream/CENARGEN/29401/1/bp172.pd

Resistência extrema a duas estirpes do Potato virus Y (PVY) de batata transgênica, cv. Achat, expressando o gene da capa protéica do PVYO

The coat protein (CP) gene of the potato virus Y strain “o” (PVYO) was introduced into potato, cultivar Achat, via Agrobacterium tumefaciens-mediated transformation. Sixty three putative transgenic lines were challenged against the Brazilian strains PVY-OBR and PVY-NBR. An extremely resistant phenotype, against the two strains, was observed in one line, denominated 1P. No symptoms or positive ELISA results were observed in 16 challenged plants from this line. Another clone, named as 63P, showed a lower level of resistance. Southern blot analysis showed five copies of the CP gene in the extremely resistant line and at least three copies in the other resistant line. The stability of the integrated transgenes in the extreme resistant line was examined during several in vitro multiplications over a period of three years, with no modification in the Southern pattern was observed. The stability of the transgenes, the absence of primary infections and the relatively broad spectrum of resistance suggest that the extremely resistant line obtained in this work can be useful for agricultural purposes.O gene da capa protéica (CP) do Potato virus Y estirpe “o”, foi introduzido em batata cultivar Achat, via Agrobacterium tumefaciens. Sessenta e três linhas possivelmente transgênicas foram desafiadas com as estirpes brasileiras PVY-OBR e PVY-NBR. Uma linha apresentou extrema resistência às duas estirpes inoculadas, e foi denominado clone 1P. Não foram observados sintomas sistêmicos de infecção e as plantas foram negativas em Elisa. Outra linha, denominada clone 63P, mostrou algum nível de resistência. Análises por Southern blot indicaram a presença de pelo menos cinco cópias do gen CP no clone 1P e pelo menos três cópias no clone 63P. A estabilidade do gene introduzido no clone 1P foi avaliada durante três anos, após várias multiplicações in vitro. Não foram observadas mudanças no padrão do Southern blot. A estabilidade do transgene, na ausência de infecções primárias e relativo largo espectro de resistência sugerem que o clone 1P pode ser utilizado para fins comerciais.Fil: Romano, Eduardo. Embrapa Recursos Genéticos; BrasilFil: Ferreira, Adriana T.. Embrapa Hortaliças; BrasilFil: Dusi, André N.. Embrapa Hortaliças; BrasilFil: Proite, Karina. Embrapa Recursos Genéticos; BrasilFil: Buso, Jose A.. Embrapa Hortaliças; BrasilFil: Avila, Antonio C.. Embrapa Hortaliças; BrasilFil: Nishijima, Marta L.. Embrapa Hortaliças; BrasilFil: Nascimento, Adriana S.. Embrapa Hortaliças; BrasilFil: Bravo Almonacid, Fernando Felix. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Mentaberry, Alejandro Nestor. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Monte, Damares. Embrapa Recursos Genéticos; BrasilFil: Campos, Magnolia A.. Embrapa Recursos Genéticos; BrasilFil: Melo, Paulo Eduardo. Embrapa Hortaliças; BrasilFil: Cattony, Monica K.. No especifica;Fil: Torres, Antonio C.. Embrapa Hortaliças; Brasi

Post-infection development and histopathology of Meloidogyne arenaria race 1 on Arachis spp.

Made available in DSpace on 2018-05-30T00:55:02Z (GMT). No. of bitstreams: 1 SP19647ID30943.pdf: 1870923 bytes, checksum: 271ee8b1af0b453bfa5079eae60f101b (MD5) Previous issue date: 2009-02-0

Sequence analysis from rescued plasmids from soybean obtained by biolistics suggest that multiple copies of the transgenes may originate by replication of exogenous DNA.

Na publicação: Romano, E.; Rech, E.; Aragão, F

BAC libraries construction from the ancestral diploid genomes of the allotetraploid cultivated peanut.

Made available in DSpace on 2018-05-31T00:42:54Z (GMT). No. of bitstreams: 1 SP19675ID30998.pdf: 743859 bytes, checksum: c6af6aed902ee8b7fd13e811390959d0 (MD5) Previous issue date: 2009-02-1

Fast ground state manipulation of neutral atoms in microscopic optical traps

We demonstrate Rabi flopping at MHz rates between ground hyperfine states of neutral 87Rb atoms that are trapped in two micron sized optical traps. Using tightly focused laser beams we demonstrate high fidelity, site specific Rabi rotations with cross talk on neighboring sites separated by 8  μm at the level of 10−3. Ramsey spectroscopy is used to measure a dephasing time of 870  μs, which is ≈5000  times longer than the time for a π/2 pulse

Extreme resistance to two Brazilian strains of potato virus Y (PVY) in transgenic potato, cv. Achat, expressing the PVY coat protein.

The coat protein (CP) gene of the potato virus Y strain “o” (PVYO) was introduced into potato, cultivar Achat, via Agrobacterium tumefaciens-mediated transformation. Sixty three putative transgenic lines were challenged against the Brazilian strains PVY-OBR and PVY-NBR. An extremely resistant phenotype, against the two strains, was observed in one line, denominated 1P. No symptoms or positive ELISA results were observed in 16 challenged plants from this line. Another clone, named as 63P, showed a lower level of resistance. Southern blot analysis showed five copies of the CP gene in the extremely resistant line and at least three copies in the other resistant line. The stability of the integrated transgenes in the extreme resistant line was examined during several in vitro multiplications over a period of three years, with no modification in the Southern pattern was observed. The stability of the transgenes, the absence of primary infections and the relatively broad spectrum of resistance suggest that the extremely resistant line obtained in this work can be useful for agricultural purposes