Abóbora-gila (cucurbita ficifolia), uma hortaliça pouco convencional cultivada no Rio Grande do Sul.

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Gene Transfer of Engineered Calmodulin Alleviates Ventricular Arrhythmias in a Calsequestrin-Associated Mouse Model of Catecholaminergic Polymorphic Ventricular Tachycardia

Catecholaminergic polymorphic ventricular tachycardia (CPVT) is a familial arrhythmogenic syndrome characterized by sudden death. There are several genetic forms of CPVT associated with mutations in genes encoding the cardiac ryanodine receptor (RyR2) and its auxiliary proteins including calsequestrin (CASQ2) and calmodulin (CaM). It has been suggested that impairment of the ability of RyR2 to stay closed (ie, refractory) during diastole may be a common mechanism for these diseases. Here, we explore the possibility of engineering CaM variants that normalize abbreviated RyR2 refractoriness for subsequent viral-mediated delivery to alleviate arrhythmias in non-CaM-related CPVT

Distância genética entre variedades crioulas de Capsicum chinense Genetic distance in Capsicum chinense landraces.

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TRANSCRANIAL DIRECT CURRENT STIMULATION ENHANCES SUCKING OF A LIQUID BOLUS IN HEALTHY HUMANS

BACKGROUND: Transcranial direct current stimulation (tDCS) is a non-invasive technique used for modulating cortical excitability in vivo in humans. Here we evaluated the effect of tDCS on behavioral and electrophysiological aspects of physiological sucking and swallowing. METHODS: Twelve healthy subjects underwent three tDCS sessions (anodal, cathodal and sham stimulation) on separate days in a double-blind randomized order. The active electrode was placed over the right swallowing motor cortex. Repeated sucking and swallowing acts were performed at baseline and at 15 and 60 min after each tDCS session and the mean liquid bolus volume ingested at each time point was measured. We also calculated average values of the following electrophysiological parameters: 1) area and 2) duration of the rectified EMG signal from the suprahyoid/submental muscles related to the sucking and swallowing phases; 3) EMG peak amplitude for the sucking and swallowing phases; 4) area and peak amplitude of the laryngeal-pharyngeal mechanogram; 5) oropharyngeal delay. RESULTS: The volume of the ingested bolus significantly increased (by an average of about 30% compared with the baseline value) both at 15 and at 60 min after the end of anodal tDCS. The electrophysiological evaluation after anodal tDCS showed a significant increase in area and duration of the sucking phase-related EMG signal. CONCLUSIONS: Anodal tDCS leads to stronger sucking of a liquid bolus in healthy subjects, likely by increasing recruitment of cortical areas of the swallowing network. This finding might open up interesting perspectives for the treatment of patients suffering from dysphagia due to various pathological conditions

Transferibilidade de marcadores microssatélites de coco (Cocos nucifera) para butiá (Butia odorata)

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Caracterização molecular de variedades crioulas de pimentas (Capsicum baccatum) com marcadores microssatélites.

Atualmente, muitas variedades crioulas de Capsicum baccatum são cultivadas pelos agricultores no Brasil. Essas populações são fontes de genes para tolerância ou resistência aos estresses bióticos e abióticos. Porém, grande parte dessas variedades está sendo perdida devido à erosão genética causada pela substituição de cultivares. A fim de conservar e caracterizar as variedades crioulas, a Embrapa Clima Temperados mantém, desde 2002, um Banco ativo de Germoplasma de Capsicum. Com objetivo de caracterizar variedades de C. baccatum com marcadores meleculares de microssatélites, foram analisados vinte acessos que integram o acervo do Banco Ativo Germoplasma de Capsicum da Embrapa Clima Temperado