Caspase-independent programmed cell death triggers Ca2PO4 deposition in an in vitro model of nephrocalcinosis

We provide evidence of caspase-independent cell death triggering the calcification process in GDNF-silenced HK-2 cells

A Study on Secret Key Rate in Wideband Rice Channel

Standard cryptography is expected to poorly fit IoT applications and services, as IoT devices can hardly cope with the computational complexity often required to run encryption algorithms. In this framework, physical layer security is often claimed as an effective solution to enforce secrecy in IoT systems. It relies on wireless channel characteristics to provide a mechanism for secure communications, with or even without cryptography. Among the different possibilities, an interesting solution aims at exploiting the random-like nature of the wireless channel to let the legitimate users agree on a secret key, simultaneously limiting the eavesdropping threat thanks to the spatial decorrelation properties of the wireless channel. The actual reliability of the channel-based key generation process depends on several parameters, as the actual correlation between the channel samples gathered by the users and the noise always affecting the wireless communications. The sensitivity of the key generation process can be expressed by the secrecy key rate, which represents the maximum number of secret bits that can be achieved from each channel observation. In this work, the secrecy key rate value is computed by means of simulations carried out under different working conditions in order to investigate the impact of major channel parameters on the SKR values. In contrast to previous works, the secrecy key rate is computed under a line-of-sight wireless channel and considering different correlation levels between the legitimate users and the eavesdropper

Prioritized motion-force control of constrained fully-actuated robots: "Task Space Inverse Dynamics"

Pre-print submitted to "Robotics and Autonomous Systems"We present a new framework for prioritized multi-task motion-force control of fully-actuated robots. This work is established on a careful review and comparison of the state of the art. Some control frameworks are not optimal, that is they do not find the optimal solution for the secondary tasks. Other frameworks are optimal, but they tackle the control problem at kinematic level, hence they neglect the robot dynamics and they do not allow for force control. Still other frameworks are optimal and consider force control, but they are computationally less efficient than ours. Our final claim is that, for fully-actuated robots, computing the operational-space inverse dynamics is equivalent to computing the inverse kinematics (at acceleration level) and then the joint-space inverse dynamics. Thanks to this fact, our control framework can efficiently compute the optimal solution by decoupling kinematics and dynamics of the robot. We take into account: motion and force control, soft and rigid contacts, free and constrained robots. Tests in simulation validate our control framework, comparing it with other state-of-the-art equivalent frameworks and showing remarkable improvements in optimality and efficiency

Feasibility of laparoscopic portal vein ligation prior to major hepatectomy

AbstractBackground. Patients noted to have an inadequate future liver remnant on pre operative volumetric assessment are considered to be candidates for portal vein embolization (PVE). A subset of patients undergo laparoscopic intervention prior to PVE for staging purposes or to address the primary in Stage IV colon cancer. These patients usually undergo PVE as a subsequent additional procedure by the transhepatic route. The aim of this study was to assess the feasibility of portal vein ligation by the laparoscopic approach in suitable patients. Materials and methods. A retrospective review of a prospectively maintained database was performed to identify patients that underwent laparoscopic portal vein ligation (LPVL). The demographic, clinical, radiographic, operative and volumetric details were collected to determine the feasibility of portal vein ligation. Results. A total of nine patients underwent LPVL as part of a two stage procedure in preparation for subsequent major hepatectomy. With a median age of 67 yrs, the diagnoses included: colorectal metastasis (five patients), cholangiocarcinoma (three patients) and hepatocellular carcinoma (one patient). The ligation involved the right portal vein in all and was performed with silk ligature (seven patients) and clips (two patients). Volumetric data was available in six patients which showed a mean increase from 209.1 cc±97.76 to 495.83 cc±310.91 (increase by 181.5%) In two patients, inadequate hypertrophy mandated later embolization by percutaneous technique. Five patients underwent subsequent major hepatic resection as planned. The remaining four patients were noted to have progression of disease that precluded the planned procedure. There were no complications associated with LPVL. Conclusions. LPVL is feasible and can be safely performed. In a select group of patients, it may be considered as an alternative to subsequent embolization and thereby potentially absolve the need for an additional procedure with its attendant complications

Trafficking properties of plasmacytoid dendritic cells in health and disease.

Plasmacytoid dendritic cells (PDCs) represent a subset of circulating leukocytes characterized by the ability to release high levels of type I interferon (IFN). Under homeostatic conditions PDCs are confined to primary and secondary lymphoid organs. This is consistent with the restricted profile of functional chemotactic receptors expressed by circulating PDCs (i.e. CXCR4 and ChemR23). Accumulation of PDCs in non-lymphoid tissue is, however, observed in certain autoimmune diseases, allergic reactions and tumors. Indeed, PDCs are now considered to be involved in the pathogenesis of diseases characterized by a type I IFN-signature and are considered as a promising target for new intervention strategies. Here, current knowledge of the molecular mechanisms involved in the recruitment of PDCs under homeostatic and pathological conditions are summarized

Role of Atypical Chemokine Receptors in Microglial Activation and Polarization.

Inflammatory reactions occurring in the central nervous system (CNS), known as neuroinflammation, are key components of the pathogenic mechanisms underlying several neurological diseases. The chemokine system plays a crucial role in the recruitment and activation of immune and non-immune cells in the brain, as well as in the regulation of microglia phenotype and function. Chemokines belong to a heterogeneous family of chemotactic agonists that signal through the interaction with G protein-coupled receptors (GPCRs). Recently, a small subset of chemokine receptors, now identified as “atypical chemokine receptors” (ACKRs), has been described. These receptors lack classic GPCR signaling and chemotactic activity and are believed to limit inflammation through their ability to scavenge chemokines at the inflammatory sites. Recent studies have highlighted a role for ACKRs in neuroinflammation. However, in the CNS, the role of ACKRs seems to be more complex than the simple control of inflammation. For instance, CXCR7/ACKR3 was shown to control T cell trafficking through the regulation of CXCL12 internalization at CNS endothelial barriers. Furthermore, D6/ACKR2 KO mice were protected in a model of experimental autoimmune encephalomyelitis (EAE). D6/ACKR2 KO showed an abnormal accumulation of dendritic cells at the immunization and a subsequent impairment in T cell priming. Finally, CCRL2, an ACKR-related protein, was shown to play a role in the control of the resolution phase of EAE. Indeed, CCRL2 KO mice showed exacerbated, non- resolving disease with protracted inflammation and increased demyelination. This phenotype was associated with increased microglia and macrophage activation markers and imbalanced M1 vs. M2 polarization. This review will summarize the current knowledge on the role of the ACKRs in neuroinflammation with a particular attention to their role in microglial polarization and function

Prioritized Optimal Control

Pre-print of the paper presented at Robotics and Automation (ICRA), IEEE International Conference on, Hong Kong, China, 2014This paper presents a new technique to control highly redundant mechanical systems, such as humanoid robots. We take inspiration from two approaches. Prioritized control is a widespread multi-task technique in robotics and animation: tasks have strict priorities and they are satisfied only as long as they do not conflict with any higher-priority task. Optimal control instead formulates an optimization problem whose solution is either a feedback control policy or a feedforward trajectory of control inputs. We introduce strict priorities in multi-task optimal control problems, as an alternative to weighting task errors proportionally to their importance. This ensures the respect of the specified priorities, while avoiding numerical conditioning issues. We compared our approach with both prioritized control and optimal control with tests on a simulated robot with 11 degrees of freedom

Contributions to the linear and nonlinear theory of the beam-plasma interaction

We focus our attention on some relevant aspects of the beam-plasma instability in order to refine some features of the linear and nonlinear dynamics. After a re-Analysis of the Poisson equation and of the assumption dealing with the background plasma in the form of a linear dielectric, we study the non-perturbative properties of the linear dispersion relation, showing the necessity for a better characterization of the mode growth rate in those flat regions of the distribution function where the Landau formula is no longer predictive. We then upgrade the original-body approach in O'Neil et al. (Phys. Fluids, vol. 14, 1971, pp. 1204-1212), in order to include a return current in the background plasma. This correction term is responsible for smaller saturation levels and growth rates of the Langmuir modes, as result of the energy density transferred to the plasma via the return current. Finally, we include friction effects, as those due to the collective influence of all the plasma charges on the motion of the beam particles. The resulting force induces a progressive resonance detuning, because particles are losing energy and decreasing their velocity. This friction phenomenon gives rise to a deformation of the distribution function, associated with a significant growth of the less energetic particle population. The merit of this work is to show how a fine analysis of the beam-plasma instability outlines a number of subtleties about the linear, intermediate and late dynamics which can be of relevance when such a system is addressed as a paradigm to describe relevant nonlinear wave-particle phenomena (Chen Zonca, Rev. Mod. Phys., vol. 88, 2016, 015008)