Computerized respiratory sound analysis in people with dementia: a first-step towards diagnosis and monitoring of respiratory conditions

Computerized respiratory sound analysis has been shown to be an objective and reliable way to assess respiratory diseases. However, its application in non-collaborative populations, such as people with dementia, is still unknown. Therefore this study aimed to characterize normal and adventitious respiratory sounds (NRS; ARS) in older people with and without dementia. A cross-sectional study including two groups of 30 subjects with dementia and 30 subjects without dementia was performed. Digital auscultation was used to record NRS and ARS per breathing-phase (inspiration/expiration) at trachea and thorax. Frequency at percentiles 25, 50 and 75, frequency at maximum-intensity, maximum-intensity (I max) and mean-intensity (I mean) characterized NRS. Crackle number, frequency, initial-deflection-width, 2cycle-duration, and largest-deflection-width and wheeze number, frequency and occupation-rate characterized ARS. Groups were similar in socio-demographics, except for anthropometrics. No significant differences were found between groups in NRS frequency or ARS at trachea or thorax. Significant lower I max (inspiration: 36.88(29.42;39.92) versus 39.84(36.50;44.17) p  =  0.007; expiration: 34.51(32.06;38.87) versus 42.33(36.92;44.98) p  <  0.001) and I mean (inspiration: 15.23(12.08;18.60) versus 18.93(15.64;21.82) p  =  0.003 and expiration: 14.57(12.08;18.30) versus 18.87(15.64;21.44) p  =  0.001) at trachea and higher I mean (inspiration: 17.29(16.04;19.31) versus 16.45(15.05; 18.79) p  =  0.005 and expiration: 16.71(15.31;18.56) versus 16.38(14.40;17.85) p  =  0.011) at thorax were found in subjects with dementia when compared with subjects without dementia. To conclude, people with and without dementia had similar NRS and ARS characteristics, except for NRS intensity. Computerized respiratory sound analysis was feasible in a non-collaborative population. Further research is needed to enhance the use of respiratory acoustics in non-collaborative populations, with strong potential to be applied in different settings for diagnosis and monitoring purposes

L’entretien d’équipe pédagogique

info:eu-repo/semantics/publishe

Differential regulation of ESAG transcripts in Trypanosoma brucei.

In Trypanosoma brucei, several genes termed ESAGs for expression site-associated genes are contained within the polycistronic transcription units of the VSG gene, and their transcription is coordinately regulated during the parasite life-cycle. Since the VSG mRNA is characterized by a drastic destabilization under conditions where translation is inhibited, we examined if this post-transcriptional control also applies to the ESAG mRNAs. While the ESAG 7/6 mRNA behaved like the VSG mRNA, the ESAG 8 and ESAG 3 mRNAs did not. We ascribe this differential behaviour to the residual transcription that still occurs only in the ESAG 7/6 region of the VSG unit under conditions where this unit is down-regulated.Journal ArticleResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/publishe