12,268 research outputs found
The photosynthetic electron transport chain of oxygenic photosynthesis
Oxygenic photosynthesis, performed by plants, algae, and cyanobacteria, is the major route by which solar energy is converted into chemical energy on earth. This process provides the essentials (e.g., food and fuels) for humans to survive and is responsible for oxygenating the earth's atmosphere, which allowed the evolution of multicellular life. Photon energy is harvested during the so-called âlight reactionsâ and used to extract electrons from water, which are then transported through an electron transport chainâin a type of bioelectric currentâthat is coupled to the movement of protons across the thylakoid membrane, storing energy in the form of a proton electrochemical gradient. The net result of the light reactions is the synthesis of adenosine triphosphate and reduced nicotinamide adenine dinucleotide phosphate, which are used by the âdarkâ or âlight-independentâ reactions to convert carbon dioxide into carbohydrates in the CalvinâBenson cycle. In this study, we summarize the structure and function of the main redox-active proteins involved in electron transfer and highlight some recent developments aiming to enhance the efficiency and robustness of the light reactions
Development of in/outflow boundary conditions for MPM simulation of uniform and non-uniform open channel flows
This paper describes the development and application of inflow and outflow boundary conditions (BCs) for the material point method (MPM) in order to simulate fluid flow problems. This corresponds to velocity and pressure controlled BCs. Due to the coupled Lagrangian and Eulerian description of the fluid motion in MPM it is necessary to add and remove material points, with appropriate kinematic properties, to and from the computational domain. The newly-developed BCs have been used to simulate uniform open channel flow and the phenomenon of free overfall in open channels, which is transient conditions leading to non-uniform flow due to a sudden bed level drop. It is shown that the numerical results predict well the flow geometry including end depth ratio, pressure distribution and accelerations, therefore the velocities and displacements.Fundamental Research Funds for the Central Universities (2017B12214) of the Ministry of Education of China,
NWO Project MPM-FLOW: Understanding flowslides in flood defences (Grant No. 13889
Axin2 as regulatory and therapeutic target in newborn brain injury and remyelination.
Permanent damage to white matter tracts, comprising axons and myelinating oligodendrocytes, is an important component of brain injuries of the newborn that cause cerebral palsy and cognitive disabilities, as well as multiple sclerosis in adults. However, regulatory factors relevant in human developmental myelin disorders and in myelin regeneration are unclear. We found that AXIN2 was expressed in immature oligodendrocyte progenitor cells (OLPs) in white matter lesions of human newborns with neonatal hypoxic-ischemic and gliotic brain damage, as well as in active multiple sclerosis lesions in adults. Axin2 is a target of Wnt transcriptional activation that negatively feeds back on the pathway, promoting β-catenin degradation. We found that Axin2 function was essential for normal kinetics of remyelination. The small molecule inhibitor XAV939, which targets the enzymatic activity of tankyrase, acted to stabilize Axin2 levels in OLPs from brain and spinal cord and accelerated their differentiation and myelination after hypoxic and demyelinating injury. Together, these findings indicate that Axin2 is an essential regulator of remyelination and that it might serve as a pharmacological checkpoint in this process
Deep Burst Denoising
Noise is an inherent issue of low-light image capture, one which is
exacerbated on mobile devices due to their narrow apertures and small sensors.
One strategy for mitigating noise in a low-light situation is to increase the
shutter time of the camera, thus allowing each photosite to integrate more
light and decrease noise variance. However, there are two downsides of long
exposures: (a) bright regions can exceed the sensor range, and (b) camera and
scene motion will result in blurred images. Another way of gathering more light
is to capture multiple short (thus noisy) frames in a "burst" and intelligently
integrate the content, thus avoiding the above downsides. In this paper, we use
the burst-capture strategy and implement the intelligent integration via a
recurrent fully convolutional deep neural net (CNN). We build our novel,
multiframe architecture to be a simple addition to any single frame denoising
model, and design to handle an arbitrary number of noisy input frames. We show
that it achieves state of the art denoising results on our burst dataset,
improving on the best published multi-frame techniques, such as VBM4D and
FlexISP. Finally, we explore other applications of image enhancement by
integrating content from multiple frames and demonstrate that our DNN
architecture generalizes well to image super-resolution
Chlorophyll f synthesis by a super-rogue photosystem II complex
Certain cyanobacteria synthesize chlorophyll molecules (Chl d and Chl f) that absorb in the far-red region of the solar spectrum, thereby extending the spectral range of photosynthetically active radiation1,2. The synthesis and introduction of these far-red chlorophylls into the photosynthetic apparatus of plants might improve the efficiency of oxygenic photosynthesis, especially in far-red enriched environments, such as in the lower regions of the canopy3. Production of Chl f requires the ChlF subunit, also known as PsbA4 (ref. 4) or super-rogue D1 (ref. 5), a paralogue of the D1 subunit of photosystem II (PSII) which, together with D2, bind cofactors involved in the light-driven oxidation of water. Current ideas suggest that ChlF oxidizes Chl a to Chl f in a homodimeric ChlF reaction centre (RC) complex and represents a missing link in the evolution of the heterodimeric D1/D2 RC of PSII (refs. 4,6). However, unambiguous biochemical support for this proposal is lacking. Here, we show that ChlF can substitute for D1 to form modified PSII complexes capable of producing Chl f. Remarkably, mutation of just two residues in D1 converts oxygen-evolving PSII into a Chl f synthase. Overall, we have identified a new class of PSII complex, which we term âsuper-rogueâ PSII, with an unexpected role in pigment biosynthesis rather than water oxidation
Achieving Îť/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator
In this report, a Ti:Sapphire oscillator was utilized to realize synchronization-free stimulated emission depletion (STED) microscopy. With pump power of 4.6 W and sample irradiance of 310 mW, we achieved super-resolution as high as 71 nm. With synchronization-free STED, we imaged 200 nm nanospheres as well as all three cytoskeletal elements (microtubules, intermediate filaments, and actin filaments), clearly demonstrating the resolving power of synchronization-free STED over conventional diffraction limited imaging. It also allowed us to discover that, Dylight 650, exhibits improved performance over ATTO647N, a fluorophore frequently used in STED. Furthermore, we applied synchronization-free STED to image fluorescently-labeled intracellular viral RNA granules, which otherwise cannot be differentiated by confocal microscopy. Thanks to the widely available Ti:Sapphire oscillators in multiphoton imaging system, this work suggests easier access to setup super-resolution microscope via the synchronization-free STED. Š 2012 Liu et al
Cortical depth dependent functional responses in humans at 7T: improved specificity with 3D GRASE
Ultra high fields (7T and above) allow functional imaging with high contrast-to-noise ratios and improved spatial resolution. This, along with improved hardware and imaging techniques, allow investigating columnar and laminar functional responses. Using gradient-echo (GE) (T2* weighted) based sequences, layer specific responses have been recorded from human (and animal) primary visual areas. However, their increased sensitivity to large surface veins potentially clouds detecting and interpreting layer specific responses. Conversely, spin-echo (SE) (T2 weighted) sequences are less sensitive to large veins and have been used to map cortical columns in humans. T2 weighted 3D GRASE with inner volume selection provides high isotropic resolution over extended volumes, overcoming some of the many technical limitations of conventional 2D SE-EPI, whereby making layer specific investigations feasible. Further, the demonstration of columnar level specificity with 3D GRASE, despite contributions from both stimulated echoes and conventional T2 contrast, has made it an attractive alternative over 2D SE-EPI. Here, we assess the spatial specificity of cortical depth dependent 3D GRASE functional responses in human V1 and hMT by comparing it to GE responses. In doing so we demonstrate that 3D GRASE is less sensitive to contributions from large veins in superficial layers, while showing increased specificity (functional tuning) throughout the cortex compared to GE
Propagation of an Earth-directed coronal mass ejection in three dimensions
Solar coronal mass ejections (CMEs) are the most significant drivers of
adverse space weather at Earth, but the physics governing their propagation
through the heliosphere is not well understood. While stereoscopic imaging of
CMEs with the Solar Terrestrial Relations Observatory (STEREO) has provided
some insight into their three-dimensional (3D) propagation, the mechanisms
governing their evolution remain unclear due to difficulties in reconstructing
their true 3D structure. Here we use a new elliptical tie-pointing technique to
reconstruct a full CME front in 3D, enabling us to quantify its deflected
trajectory from high latitudes along the ecliptic, and measure its increasing
angular width and propagation from 2-46 solar radii (approximately 0.2 AU).
Beyond 7 solar radii, we show that its motion is determined by an aerodynamic
drag in the solar wind and, using our reconstruction as input for a 3D
magnetohydrodynamic simulation, we determine an accurate arrival time at the
Lagrangian L1 point near Earth.Comment: 5 figures, 2 supplementary movie
T-cell subpopulations ιβ and γδ in cord blood of very preterm infants : The influence of intrauterine infection
Open Access: This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are creditedPreterm infants are very susceptible to infections. Immune response mechanisms in this group of patients and factors that influence cord blood mononuclear cell populations remain poorly understood and are considered insufficient. However, competent immune functions of the cord blood mononuclear cells are also described. The aim of this work was to evaluate the T-cell population (CD3+) with its subpopulations bearing T-cell receptor (TCR) ιβ or TCR γδ in the cord blood of preterm infants born before 32 weeks of gestation by mothers with or without an intrauterine infection. Being a pilot study, it also aimed at feasibility check and assessment of an expected effect size. The cord blood samples of 46 infants age were subjected to direct immunofluorescent staining with monoclonal antibodies and then analyzed by flow cytometry. The percentage of CD3+ cells in neonates born by mothers with diagnosis of intrauterine infection was significantly lower than in neonates born by mothers without infection (p = 0.005; Mann-Whitney U test). The number of cells did not differ between groups. Infection present in the mother did not have an influence on the TCR ιβ or TCR γδ subpopulations. Our study contributes to a better understanding of preterm infants' immune mechanisms, and sets the stage for further investigations.Peer reviewedFinal Published versio
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