3,843 research outputs found
Cytometric analysis, genetic manipulation and antibiotic selection of the snail embryonic cell line Bge from Biomphalaria glabrata, the intermediate host of Schistosoma mansoni.
The invertebrate cell line, Bge, from embryos of the snail Biomphalaria glabrata, remains to date the only established cell line from any species of the Phylum Mollusca. Since its establishment in 1976 by Eder Hansen, few studies have focused on profiling its cytometrics, growth characteristics or sensitivity to xenobiotics. Bge cells are reputed to be challenging to propagate and maintain. Therefore, even though this cell line is a noteworthy resource, it has not been studied widely. With growing interest in functional genomics, including genetic transformation, to elucidate molecular aspects of the snail intermediate hosts responsible for transmission of schistosomiasis, and aiming to enhance the convenience of maintenance of this molluscan cell line, we deployed the xCELLigene real time approach to study Bge cells. Doubling times for three isolates of Bge, termed CB, SL and UK, were longer than for mammalian cell lines - longer than 40 h in complete Bge medium supplemented with 7% fetal bovine serum at 25 °C, ranging from ∼42 h to ∼157 h when 40,000 cells were seeded. To assess the potential of the cells for genetic transformation, antibiotic selection was explored. Bge cells were sensitive to the aminonucleoside antibiotic puromycin (from Streptomyces alboniger) from 5 μg/ml to 200 ng/ml, displaying a half maximal inhibitory concentration (IC50) of ∼1.91 μg/ml. Sensitivity to puromycin, and a relatively quick kill time (<48 h in 5 μg/ml) facilitated use of this antibiotic, together with the cognate resistance gene (puromycin N-acetyl-transferase) for selection of Bge cells transformed with the PAC gene (puroR). Bge cells transfected with a plasmid encoding puroR were partially rescued when cultured in the presence of 5 μg/ml of puromycin. These findings pave the way for the development of functional genomic tools applied to the host-parasite interaction during schistosomiasis and neglected tropical trematodiases at large
Basal-plane Incommensurate Phases in HCP Structures
An Ising model with competing interaction is used to study the appearance of
incommensurate phases in the basal plane of an hexagonal closed-packed
structure. The calculated mean-field phase diagram reveals various
1q-incommensurate and lock-in phases. The results are applied to explain the
basal-plane incommensurate phase in some compounds of the A'A"BX_4 family, like
K_2MoO_4, K_2WO_4, Rb_2WO4 and to describe the sequence of high-temperature
phase transitions in other compounds of this family.Comment: 8 pages, RevTeX + 4 ps figure
A integração Google Earth-SIG-Servidor de mapas e o monitoramento ambiental.
bitstream/item/79549/1/circ-183.pd
The Estimation of the Effective Centre of Mass Energy in q-qbar-gamma Events from DELPHI
The photon radiation in the initial state lowers the energy available for the
ee collisions; this effect is particularly important at LEP2 energies
(above the mass of the Z boson). Being aligned to the beam direction, such
initial state radiation is mostly undetected. This article describes the
procedure used by the DELPHI experiment at LEP to estimate the effective
centre-of-mass energy in hadronic events collected at energies above the Z
peak. Typical resolutions ranging from 2 to 3 GeV on the effective
center-of-mass energy are achieved, depending on the event topology.Comment: 12 pages, 6 figure
Cerenkov angle and charge reconstruction with the RICH detector of the AMS experiment
The Alpha Magnetic Spectrometer (AMS) experiment to be installed on the
International Space Station (ISS) will be equipped with a proximity focusing
Ring Imaging Cerenkov (RICH) detector, for measurements of particle electric
charge and velocity. In this note, two possible methods for reconstructing the
Cerenkov angle and the electric charge with the RICH, are discussed. A
Likelihood method for the Cerenkov angle reconstruction was applied leading to
a velocity determination for protons with a resolution of around 0.1%. The
existence of a large fraction of background photons which can vary from event
to event, implied a charge reconstruction method based on an overall efficiency
estimation on an event-by-event basis.Comment: Proceedings submitted to RICH 2002 (Pylos-Greece
Exploring the tensile response in small carbon fibre composite bundles
Small composite bundles, AS4 carbon fibre epoxy, with a restricted number of reinforcing fibres, ca. 20, showed a progressive failure when tested in tension. In-situ acoustic emission observations under tensile load reveal that numerous fibres fail before ultimate failure of the small composite bundle, suggesting that isolated and individual fibre failures occur without compromising the integrity of the neighboring fibres or the small composite bundle’s overall mechanical performance. The average strength of the carbon fibres in small composite bundles was 9.6% higher than in standard lab-scale composite specimens using the same fibre type
LATTES: a novel detector concept for a gamma-ray experiment in the Southern hemisphere
The Large Array Telescope for Tracking Energetic Sources (LATTES), is a novel
concept for an array of hybrid EAS array detectors, composed of a Resistive
Plate Counter array coupled to a Water Cherenkov Detector, planned to cover
gamma rays from less than 100 GeV up to 100 TeVs. This experiment, to be
installed at high altitude in South America, could cover the existing gap in
sensitivity between satellite and ground arrays.
The low energy threshold, large duty cycle and wide field of view of LATTES
makes it a powerful tool to detect transient phenomena and perform long term
observations of variable sources. Moreover, given its characteristics, it would
be fully complementary to the planned Cherenkov Telescope Array (CTA) as it
would be able to issue alerts.
In this talk, a description of its main features and capabilities, as well as
results on its expected performance, and sensitivity, will be presented.Comment: Proceedings of the 35th International Cosmic Ray Conference
(ICRC2017), Busan, South Korea. Presented by R. Concei\c{c}\~{a}o. 8 pages;
v2: correct affiliation + journal referenc
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