A KARYOMETRIC STUDY OF HEAT-SHOCKED HELA-CELLS

HeLa cells exposed to heat shock at temperatures of 40-degrees-C and 43-degrees-C for 1 hour and also cells 4h after returning to control conditions (37-degrees-C) were studied comparatively with respect to nuclear and nucleolar sizes, using karyometric procedures. Some alterations especially involving nucleolar areas were found to be promoted by the shock, though mostly detectable during the post-shock period held at control temperature. These alterations when elicited by the shock at 43-degrees-C were assumed to be associated with transcriptional activities involved in recovery of the ordinary cellular activities, whereas those related to the shock at 40-degrees-C were suggested to be due to karyoskeletal proteins appearing late with the stress and which could be mechanically restraining the nucleolar area increase. These proteins also appear in the HeLa cells exposed to the heat shock at 43-degrees-C and equally seem to affect the increase in nucleolar area in comparison to nuclear area, however they are disassembled when the cells are returned to control temperature.16116117

ASSESSMENT OF PATHOGENIC EFFECTS OF INFECTION WITH SCHISTOSOMA-MANSONI SAMBON, 1907, AGENT OF UNISEXUAL INFECTION IN MUS-MUSCULUS

17539440

CHRONIC CHAGAS-DISEASE - UNIMPAIRED DELAYED-TYPE HYPERSENSITIVITY REACTIONS IN CONTRAST WITH HUMORAL SUPPRESSION TO TYPHOID VACCINE

322828

TRAINING AND STANDARDIZATION OF PERSONNEL INVOLVED IN AN ANTHROPOMETRIC STUDY WITH SCHOOL-CHILDREN

31230331

REGENERATION OF GARLIC PLANTS (ALLIUM-SATIVUM L, CV CHONAN) VIA CELL-CULTURE IN LIQUID-MEDIUM

Aiming at the genetic improvement of garlic cultivars, a cell suspension protocol was established which includes the induction of friable callus, establishment of cells in liquid medium, plating, regeneration, and bulb formation. Calluses of various textures from compact to friable and from green to yellowish were obtained by culturing explants excised from inner leaves of garlic bulbs on Marashig-Shoog (MS) medium with 2,4 dichlorophenoxy acetic acid (2,4-D), (1.1 mg/liter [5.0 mu M]), picloram (1.2 mg/liter [5.0 mu M]), and kinetin (2.1 mg/liter [10 mu M]). Friable callus occurred on MS-A contained 2,4-D alone (1.0 mg/liter [4.52 mu M]) and this callus was used to develop cell suspension cultures, which were maintained in liquid MS-B medium with a 2,4-D/benzyl adenine (BA) (0.5 mg/liter [2.25 mu M]: 0.5 mg/liter [2.22 mu M]) ratio. High plating efficiency was obtained on MS-C medium with different naphthalene acetic acid/BA combinations. Regeneration occurred after transfer of the caulogenic mass to MS-C medium containing 10 mg/liter (74.02 mu M) and 20 mg/liter (148.04 mu M) adenine for 60 days, followed by transfer to adenine-free medium. Plantlets transplanted to soil showed normal phenology. Shoots grown on modified MS medium supplemented with indolylbutryic acid (3.0 mg/liter [14.7 mu M]) stimulated bulb formation by 30 days in culture.30P315015

IMMUNOLOGICAL AND PARASITOLOGICAL ASPECTS OF BIOMPHALARIA-TENAGOPHILA INFECTED BY SCHISTOSOMA-MANSONI AND OTHER DIGENEA

The behavior of Biomphalaria tenagophila amoebocytes was studied in infections produced by Schistosoma mansoni and other Digenea. The resistance to superinfection was also verified in mixed infections. Data on amoebocyte phagocytic activity, on the number of amoebocytes in hemolymph, and on amoebocyte tissue ractions were obtained and eletrophoretic and imunodiffusion examinations of the hemolymph were carried out. It was concluded that the snails infected with Digenea show resistance to superinfection with S. mansoni. Apparently sporocysts are not destroyed by the action of amoebocytes. An increase in amoebocyte phagocytic activity was discovered in infected snails. Immunoeletrophoresis shows quantitative and qualitative differences in the hemolymph of the infected snails.27642142

THE EVOLUTION OF IMMUNOGLOBULINS INVOLVED IN THE IMMUNE-RESPONSE TO MICE INFECTED WITH SCHISTOSOMA-MANSONI

18213815

Total lipids and fatty acids of strains of Metarhizium anisopliae

Two growth stages, conidia (C) and mycelium (M), and two media, minimal medium (MM) and complete medium (MC), were compared in 10 strains of M. anisopliae, and two strains of M. anisopliae var, majus were similar in percentages of total lipids. Tukey test for average of lipid content. in conidia (C) and mycelia (M) cultured on minimal (MM) and complete (MC) media showed significant differences between means at the 5% level for mycelia and conidia, indicating variability in total lipid production and storage during growth. Strains 5 and 7, both variety majus, did not present sizable differences from variety anisopliae. For fatty acids, C18:1 and C18:2, oleic and linoleic, respectively, the differences were all highly significant (p=1%) with the highest means being obtained for conidia for fatty acid C18:1 and for myclelia for fatty acid C18:2.31212112

OCCURRENCE OF WILD AND HUMAN STRAINS OF SCHISTOSOMA-MANSONI IN LOWER AMAZONIA .1. STUDY IN MOLLUSKS

16529229