All-optical GeV electron bunch generation in a laser-plasma accelerator via truncated-channel injection.

We describe a simple scheme, truncated-channel injection, to inject electrons directly into the wakefield driven by a high-intensity laser pulse guided in an all-optical plasma channel. We use this approach to generate dark-current-free 1.2 GeV, 4.5% relative energy spread electron bunches with 120 TW laser pulses guided in a 110 mm-long hydrodynamic optical-field-ionized plasma channel. Our experiments and particle-in-cell simulations show that high-quality electron bunches were only obtained when the drive pulse was closely aligned with the channel axis, and was focused close to the density down ramp formed at the channel entrance. Start-to-end simulations of the channel formation, and electron injection and acceleration show that increasing the channel length to 410 mm would yield 3.65 GeV bunches, with a slice energy spread ∼5×10^{-4}

Measurement of the decay of laser-driven linear plasma wakefields.

We present measurements of the temporal decay rate of one-dimensional (1D), linear Langmuir waves excited by an ultrashort laser pulse. Langmuir waves with relative amplitudes of approximately 6% were driven by 1.7J, 50fs laser pulses in hydrogen and deuterium plasmas of density n_{e0}=8.4×10^{17}cm^{-3}. The wakefield lifetimes were measured to be τ_{wf}^{H_{2}}=(9±2) ps and τ_{wf}^{D_{2}}=(16±8) ps, respectively, for hydrogen and deuterium. The experimental results were found to be in good agreement with 2D particle-in-cell simulations. In addition to being of fundamental interest, these results are particularly relevant to the development of laser wakefield accelerators and wakefield acceleration schemes using multiple pulses, such as multipulse laser wakefield accelerators

Low-density hydrodynamic optical-field-ionized plasma channels generated with an axicon lens

We demonstrate optical guiding of high-intensity laser pulses in long, low density hydrodynamic optical-field-ionized (HOFI) plasma channels. An axicon lens is used to generate HOFI plasma channels with on-axis electron densities as low as $n_e(0) = 1.5\times 10^{17}\, \mathrm{cm}^{-3}$ and matched spot sizes in the range $20 \mu \mathrm{m} \lesssim W_M \lesssim 40 \mu \mathrm{m}$. Control of these channel parameters via adjustment of the initial cell pressure and the delay after the arrival of the channel-forming pulse is demonstrated. For laser pulses with a peak axial intensity of $4 \times 10^{17}\, \mathrm{W\,cm}^{-2}$, highly reproducible, high-quality guiding over more than 14 Rayleigh ranges is achieved at a pulse repetition rate of 5 Hz, limited by the available channel-forming laser and vacuum pumping system. Plasma channels of this type would seem to be well suited to multi-GeV laser wakefield accelerators operating in the quasi-linear regime

Meter-Scale, Conditioned Hydrodynamic Optical-Field-Ionized Plasma Channels

We demonstrate through experiments and numerical simulations that low-density, low-loss, meter-scale plasma channels can be generated by employing a conditioning laser pulse to ionize the neutral gas collar surrounding a hydrodynamic optical-field-ionized (HOFI) plasma channel. We use particle-in-cell simulations to show that the leading edge of the conditioning pulse ionizes the neutral gas collar to generate a deep, low-loss plasma channel which guides the bulk of the conditioning pulse itself as well as any subsequently injected pulses. In proof-of-principle experiments we generate conditioned HOFI (CHOFI) waveguides with axial electron densities of $n_\mathrm{e0} \approx 1 \times 10^{17} \; \mathrm{cm^{-3}}$, and a matched spot size of $26 \; \mathrm{\mu m}$. The power attenuation length of these CHOFI channels is $L_\mathrm{att} = (21 \pm 3) \; \mathrm{m}$, more than two orders of magnitude longer than achieved by HOFI channels. Hydrodynamic and particle-in-cell simulations demonstrate that meter-scale CHOFI waveguides with attenuation lengths exceeding 1 m could be generated with a total laser pulse energy of only $1.2$ J per meter of channel. The properties of CHOFI channels are ideally suited to many applications in high-intensity light-matter interactions, including multi-GeV plasma accelerator stages operating at high pulse repetition rates

Resonant excitation of plasma waves in a plasma channel

We demonstrate resonant excitation of a plasma wave by a train of short laser pulses guided in a preformed plasma channel, for parameters relevant to a plasma-modulated plasma accelerator (P-MoPA). We show experimentally that a train of N≈10 short pulses, of total energy ∼1J, can be guided through 110mm long plasma channels with on-axis densities in the range 1017-1018cm-3. The spectrum of the transmitted train is found to be strongly red shifted when the plasma period is tuned to the intratrain pulse spacing. Numerical simulations are found to be in excellent agreement with the measurements and indicate that the resonantly excited plasma waves have an amplitude in the range 3-10GVm-1, corresponding to an accelerator stage energy gain of order 1GeV

Biochemical Characterization of a Structure-Specific Resolving Enzyme from Sulfolobus islandicus Rod-Shaped Virus 2

Sulfolobus islandicus rod shaped virus 2 (SIRV2) infects the archaeon Sulfolobus islandicus at extreme temperature (70°C–80°C) and acidity (pH 3). SIRV2 encodes a Holliday junction resolving enzyme (SIRV2 Hjr) that has been proposed as a key enzyme in SIRV2 genome replication. The molecular mechanism for SIRV2 Hjr four-way junction cleavage bias, minimal requirements for four-way junction cleavage, and substrate specificity were determined. SIRV2 Hjr cleaves four-way DNA junctions with a preference for cleavage of exchange strand pairs, in contrast to host-derived resolving enzymes, suggesting fundamental differences in substrate recognition and cleavage among closely related Sulfolobus resolving enzymes. Unlike other viral resolving enzymes, such as T4 endonuclease VII or T7 endonuclease I, that cleave branched DNA replication intermediates, SIRV2 Hjr cleavage is specific to four-way DNA junctions and inactive on other branched DNA molecules. In addition, a specific interaction was detected between SIRV2 Hjr and the SIRV2 virion body coat protein (SIRV2gp26). Based on this observation, a model is proposed linking SIRV2 Hjr genome resolution to viral particle assembly

Demonstration of kilohertz operation of hydrodynamic optical-field-ionized plasma channels

We demonstrate experimentally that hydrodynamic optical-field-ionized (HOFI) plasma channels can be generated at kHz-scale pulse repetition rates, in a static gas cell and for an extended period. Using a pump-probe arrangement, we show via transverse interferometry that the properties of two HOFI channels generated 1 ms apart are essentially the same. We demonstrate that HOFI channels can be generated at a mean repetition rate of 0.4 kHz for a period of 6.5 h without degradation of the channel properties, and we determine the fluctuations in the key optical parameters of the channels in this period. Our results suggest that HOFI and conditioned HOFI channels are well suited for future high-repetition rate, multi-GeV plasma accelerator stages

Demonstration of kilohertz operation of hydrodynamic optical-field-ionized plasma channels

We demonstrate experimentally that hydrodynamic optical-field-ionized (HOFI) plasma channels can be generated at kHz-scale pulse repetition rates, in a static gas cell and for an extended period. Using a pump-probe arrangement, we show via transverse interferometry that the properties of two HOFI channels generated 1 ms apart are essentially the same. We demonstrate that HOFI channels can be generated at a mean repetition rate of 0.4 kHz for a period of 6.5 h without degradation of the channel properties, and we determine the fluctuations in the key optical parameters of the channels in this period. Our results suggest that HOFI and conditioned HOFI channels are well suited for future high-repetition rate, multi-GeV plasma accelerator stages

On the regulation of the p53 tumour suppressor, and its role in the cellular response to DNA damage.

The p53 gene is required for the normal apoptotic response of mammalian cells to DNA damage caused by ionizing radiation and DNA damaging drugs. DNA damage results in the accumulation of biologically active p53. This response is potentially lethal and is therefore highly regulated. By using both biochemical and cell biological approaches a number of discrete control pathways have been identified. These include analysis of cellular and viral proteins that bind to p53 to inactivate its function, the discovery of cells with defects in the p53 activation pathway and the analysis of an allosteric regulation of p53 function controlled by phosphorylation