The spindle position checkpoint in budding yeast: the motherly care of MEN

Mitotic exit and cytokinesis must be tightly coupled to nuclear division both in time and space in order to preserve genome stability and to ensure that daughter cells inherit the right set of chromosomes after cell division. This is achieved in budding yeast through control over a signal transduction cascade, the mitotic exit network (MEN), which is required for mitotic CDK inactivation in telophase and for cytokinesis. Current models of MEN activation emphasize on the bud as the place where most control is exerted. This review focuses on recent data that instead point to the mother cell as being the residence of key regulators of late mitotic events

The budding yeast PP2ACdc55 protein phosphatase prevents the onset of anaphase in response to morphogenetic defects

Faithful chromosome transmission requires establishment of sister chromatid cohesion during S phase, followed by its removal at anaphase onset. Sister chromatids are tethered together by cohesin, which is displaced from chromosomes through cleavage of its Mcd1 subunit by the separase protease. Separase is in turn inhibited, up to this moment, by securin. Budding yeast cells respond to morphogenetic defects by a transient arrest in G2 with high securin levels and unseparated chromatids. We show that neither securin elimination nor forced cohesin cleavage is sufficient for anaphase in these conditions, suggesting that other factors contribute to cohesion maintainance in G2. We find that the protein phosphatase PP2A bound to its regulatory subunit Cdc55 plays a key role in this process, uncovering a new function for PP2ACdc55 in controlling a noncanonical pathway of chromatid cohesion removal

The RSC chromatin-remodeling complex influences mitotic exit and adaptation to the spindle assembly checkpoint by controlling the Cdc14 phosphatase

Rsc2 promotes Cdc14 release from the nucleolus to free cells from mitotic arrest

Evaluación del estado de Salud Bucal en niños que asisten al Centro de Desarrollo Infantil Mitaí Roga N° XV de Laguna Brava- Corrientes

En la actualidad, a nivel mundial uno de los principales problemas de salud pública, son las enfermedades bucodentales. Se realizó un estudio descriptivo, de corte longitudinal, en 120 niños de 4 y 5 años de edad, de ambos sexos que asistieron al CDI Mitaí Roga N° XV de Laguna Brava-Corrientes, durante el año 2019. El objetivo fue evaluar el estado de Salud Bucal en niños de 4 y 5 años de ambos sexos que asistieron al Centro de Desarrollo Infantil Mitaí Roga N° XV. Los datos se obtuvieron mediante observación clínica y registro en Historia Clínica-dental previo consentimiento Informado. La investigación se realizó en tres etapas. La primera denominada diagnóstico inicial donde se llevó a cabo la recolección de los datos, la segunda en la cual se ejecutó el Programa de Promoción y Educación para la Salud y la tercera donde se evaluó el impacto mediante el diagnóstico final. Los resultados reflejan la disminución en el número de dientes cariados y el aumento del número de dientes obturados, así como también la disminución de los valores del índice de placa bacteriana de O’Leary, posterior a la aplicación del Programa

West Nile virus transmission. results from the integrated surveillance system in Italy, 2008 to 2015

IIn Italy a national Plan for the surveillance of imported and autochthonous human vector-borne diseases (chikungunya, dengue, Zika virus disease and West Nile virus (WNV) disease) that integrates human and veterinary (animals and vectors) surveillance, is issued and revised annually according with the observed epidemiological changes. Here we describe results of the WNV integrated veterinary and human surveillance systems in Italy from 2008 to 2015. A real time data exchange protocol is in place between the surveillance systems to rapidly identify occurrence of human and animal cases and to define and update the map of affected areas i.e. provinces during the vector activity period from June to October. WNV continues to cause severe illnesses in Italy during every transmission season, albeit cases are sporadic and the epidemiology varies by virus lineage and geographic area. The integration of surveillance activities and a multidisciplinary approach made it possible and have been fundamental in supporting implementation of and/or strengthening preventive measures aimed at reducing the risk of transmission of WNV trough blood, tissues and organ donation and to implementing further measures for vector control