Green coffee seed residue: A sustainable source of antioxidant compounds.

Made available in DSpace on 2018-05-22T00:53:03Z (GMT). No. of bitstreams: 1 ART18004.pdf: 1065695 bytes, checksum: f98193cd878bcb43d577ff3d8be34290 (MD5) Previous issue date: 2018-03-20bitstream/item/177373/1/ART18004.pd

Parthenogenesis In The Tropical Gekkonid Lizard, Nactus Arnouxii (Sauria: Gekkonidae)

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/137400/1/evo02464.pd

Cytogenetics of the Brazilian whiptail lizard Cnemidophorus littoralis (Teiidae) from a restinga area (Barra de Maricá) in Southeastern Brazil

Chromosomes of Cnemidophorus littoralis, a new species of teiid lizard recently described, were studied. The animals are from a restinga area in Barra de Maricá, RJ. The karyotype presents a diploid number of 2n = 46 chromosomes and a chromosomal sex determination mechanism of the type XX:XY. Nucleolar organizer regions, Ag-NORs, are at the sixth pair of chromosomes; there is variability of size and number of the Ag-stained nucleoli on the 50 interphase nuclei for each specimen analyzed. These nucleoli are related to NOR patterns that also demonstrated variability in size and number. This paper presents the first description of the karyotype of Cnemidophorus littoralis and of a chromosomal sex determination mechanism of the XX:XY type in the genus Cnemidophorus from Southeastern Brazil

Pharmacokinetic properties, in vitro metabolism and plasma protein binding of govaniadine an alkaloid isolated from Corydalis govaniana Wall

Made available in DSpace on 2018-11-26T17:10:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-11-30Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)National Council for Scientific and Technological DevelopmentGovaniadine (GOV) is an alkaloid isolated from Corydalis govaniana Wall. It has been reported to show a different number of biological activities including anti-urease, leishmanicidal and antinociceptive. The present study aims to characterize the GOV in vitro metabolism after incubation with rat and human liver microsomes (RLM and HLM, respectively) and to evaluate its pharmacokinetic properties. The identification of GOV metabolites was conducted by different mass analyzers: a micrOTOF II-ESI-ToF Bruker Daltonics (R) and an amaZon-SLion trap (IT) Bruker Daltonics (R). For the pharmacokinetic study of GOV in rats after intravenous administration, a LC-MS/MS method was developed and applied to. The analyses were performed using an Acquity UPLC (R) coupled to an AcquityTQD detector equipped with an ESI interface. The liver microsomal incubation resulted in new O-demethylated, di-hydroxylated and mono-hydroxylated compounds. Regarding the method validation, the calibration curve was linear over the concentration range of 2.5-3150.0 ng mL(-1), with a lower limit of quantitation (LLOQ) of 2.5 ng mL(-1). This method was successfully applied to a pharmacokinetic study. The profile was best fitted to a two-compartment model, the first phase with a high distribution rate constant (alpha) 0.139 +/- 0.086 min(-1), reflected by the short distribution half-life (t1/2 alpha) 9.2 +/- 8.9 min and the later one, with an elimination half-life (t1/2 beta) 55.1 +/- 37.9 min. The main plasma protein binding was 96.1%. This is a first report in this field and it will be useful for further development of govaniadine as a drug candidate. (C) 2016 Elsevier B.V. All rights reserved.Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Quim Fis, BR-14040903 Ribeirao Preto, SP, BrazilKings Coll London, Wolfson Ctr Age Related Dis, Guys Campus, London SE11 UL, EnglandUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Dept Principios At Nat & Toxicol, BR-14801902 Araraquara, SP, BrazilUniv Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14040901 Ribeirao Preto, SP, BrazilUniv Karachi, Int Ctr Chem & Biol Sci, HEJ Res Inst Chem, Karachi 75270, PakistanTribhuvan Univ, Amrit Sci Campus, Kathmandu, NepalUniv Estadual Paulista, Fac Ciencias Farmaceut Araraquara, Dept Principios At Nat & Toxicol, BR-14801902 Araraquara, SP, BrazilFAPESP: 2013/16496-5FAPESP: 2013/17658-9FAPESP: 2014/13192-8FAPESP: 2014/23604-1National Council for Scientific and Technological Development: 442384/201