Сравнительное изучение взаимодействия флуоресцентных красителей К-35 и К7-1045 с белками сыворотки крови крыс

Using the fluorescent spectroscopy, gel chromatography and electrophoresis in polyacrylamide gel methods interaction of fluorescent dyes K-35 and К7-1045 with rat serum proteins has been studied in the work in comparative aspect. It has been established that in serum K-35 and К7-1045 dyes bound mainly with albumin. It has been shown that K-35 dye may be used for estimating degree of rat serum albumin fullness by different ligands.В работе методами флуоресцентной спектроскопии, гель-хроматографии и электрофореза в полиакриламидном геле изучено в сравнительном аспекте взаимодействие флуоресцентных красителей К-35 и К7-1045 с белками сыворотки крови крыс. Установлено, что в сыворотке красители К-35 и К7-1045 главным образом связываются с альбумином. Показано, что краситель К-35 может быть применен для оценки степени нагруженности сывороточного альбумина крысы лигандами различного происхождения

Synthesis, structure, and spectral fluorescence properties of 1,4-di(5-phenyl-2-oxazolyl)benzene heteroanalogs

Novel organic luminophors belonging to the oxazole and oxadiazole classes of compounds have been synthesized, namely, thiophene and furan analogs of 1,4-di(5-phenyl-2-oxazolyl)benzene. The optical properties of these compunds have been studied both theoretically and experimentally. Their IR spectra have been measured and their UV absorption, fluorescence, and excitation spectra have been analyzed. Details of their electronic structure and their principal spectral fluorescence parameters have been calculated using the PPP method. Possible methods or pathways for improving the spectral fluorescence parameters of organic luminophors in these classes of compounds in the visible region of the spectrum have also been analyzed. © 1990 Plenum Publishing Corporation

Synthesis, structure, and spectral fluorescence properties of 1,4-di(5-phenyl-2-oxazolyl)benzene heteroanalogs

Novel organic luminophors belonging to the oxazole and oxadiazole classes of compounds have been synthesized, namely, thiophene and furan analogs of 1,4-di(5-phenyl-2-oxazolyl)benzene. The optical properties of these compunds have been studied both theoretically and experimentally. Their IR spectra have been measured and their UV absorption, fluorescence, and excitation spectra have been analyzed. Details of their electronic structure and their principal spectral fluorescence parameters have been calculated using the PPP method. Possible methods or pathways for improving the spectral fluorescence parameters of organic luminophors in these classes of compounds in the visible region of the spectrum have also been analyzed. © 1990 Plenum Publishing Corporation

Experimental and theoretical investigation of the reaction of 2,5-diphenyl-1,3-oxazole and 2,5-diphenyl-1,3,4-oxadiazole dimethylamino derivatives with the Vilsmeier reagent

The 2,5-diphenyl-1,3-oxazole and 2,5-diphenyl-1,3,4-oxadiazole dimethylamino deriva¬tives are widely used as organic luminophores. These compounds are found to be substi¬tuted in the ortAo-position to the dimethylamino group under conditions of the Vilsmeier- Haack reaction. The attack by the POCI3-DMF complex results in formation of the "open" iminium salt which is subjected the isomerization into tetrahydroquinazolinium salt. The substitution reaction directed to the 4 position of the oxazole ring causes formylation. The reactivity of the compounds under investigation and the reaction pathways have been studied using the quantum-chemical semi-empirical methods. The most favorable condi - tions for synthesis of the quinazolinium salts and aldehydes have been determined. The compounds obtained are of considerable interest as the water soluble high-efficiency luminophores.Диметиламинозамещенные 2,5-диарил-1,3,4-оксадиазолы и 2,5-диарил-1,3-оксазолы широко применяются в качестве органических люминофоров. Установлено, что в усло¬виях реакции Bильсмaйера-Xаака эти соединения замещаются в орто-положении к диметиламиногруппе. Атака комплексом POCI3-DMF приводит к образованию "откры¬той" иминиевой соли, которая затем изомеризуется в тетрагидрохиназолиниевую соль. Реакция замещения, направленная в положение 4 оксазольного цикла, приводит к формилированию. Реакционная способность исследуемых соединений и механизмы ре¬акций исследованы с применением квантово-химических полуэмпирических методов. Определены оптимальные условия синтеза хиназолиниевых солей и альдегидов. Полу¬ченные соединения представляют значительный интерес как водорастворимые люмино¬форы высокой эффективности

Experimental and theoretical investigation of the reaction of 2,5-diphenyl-1,3-oxazole and 2,5-diphenyl-1,3,4-oxadiazole dimethylamino derivatives with the Vilsmeier reagent

The 2,5-diphenyl-1,3-oxazole and 2,5-diphenyl-1,3,4-oxadiazole dimethylamino deriva¬tives are widely used as organic luminophores. These compounds are found to be substi¬tuted in the ortAo-position to the dimethylamino group under conditions of the Vilsmeier- Haack reaction. The attack by the POCI3-DMF complex results in formation of the "open" iminium salt which is subjected the isomerization into tetrahydroquinazolinium salt. The substitution reaction directed to the 4 position of the oxazole ring causes formylation. The reactivity of the compounds under investigation and the reaction pathways have been studied using the quantum-chemical semi-empirical methods. The most favorable condi - tions for synthesis of the quinazolinium salts and aldehydes have been determined. The compounds obtained are of considerable interest as the water soluble high-efficiency luminophores.Диметиламинозамещенные 2,5-диарил-1,3,4-оксадиазолы и 2,5-диарил-1,3-оксазолы широко применяются в качестве органических люминофоров. Установлено, что в усло¬виях реакции Bильсмaйера-Xаака эти соединения замещаются в орто-положении к диметиламиногруппе. Атака комплексом POCI3-DMF приводит к образованию "откры¬той" иминиевой соли, которая затем изомеризуется в тетрагидрохиназолиниевую соль. Реакция замещения, направленная в положение 4 оксазольного цикла, приводит к формилированию. Реакционная способность исследуемых соединений и механизмы ре¬акций исследованы с применением квантово-химических полуэмпирических методов. Определены оптимальные условия синтеза хиназолиниевых солей и альдегидов. Полу¬ченные соединения представляют значительный интерес как водорастворимые люмино¬форы высокой эффективности

Assessment of alginate hydrogel degradation in biological tissue using viscosity-sensitive fluorescent dyes

Shkand T.V, Chizh M.O., Sleta I.V., Sandomirsky B.P., Tatarets A.L., Patsenker L.D. Assessment of alginate hydrogel degradation in biological tissue using viscosity-sensitive fluorescent dyes. Methods and Applications in Fluorescence. 2016. 4(4): 1–12. DOI: 10.1088/2050-6120/4/4/044002The main goal of this study is to investigate a combination of viscosity-sensitive and viscosity-insensitive fluorescent dyes to distinguish different rheological states of hydrogel based biostructural materials and carriers in biological tissues and to assess their corresponding location areas. The research is done in the example of alginate hydrogel stained with viscosity-sensitive dyes Seta-470 and Seta-560 as well as the viscosity-insensitive dye Seta-650. These dyes absorb/emit at 469/518, 565/591 and 651/670 nm, respectively. The rheological state of the alginate, the area of the fluorescence signal and the mass of the dense alginate versus the calcium gluconate concentration utilized for alginate gelation were studied in vitro. The most pronounced change in the fluorescence signal area was found at the same concentrations of calcium gluconate (below ~1%) as the change in the alginate plaque mass. The stained alginate was also implanted in situ in rat hip and myocardium and monitored using fluorescence imaging. In summary, our data indicate that the viscosity sensitive dye in combination with the viscosity-insensitive dye allow tracking the biodegradation of the alginate hydrogel and determining the rheological state of hydrogel in biological tissue, which both should have relevance for research and clinical applications. Using this method we estimated the half-life of the dense alginate hydrogel in a rat hip to be in the order of 4 d and about 6–8 d in rat myocardium. The half-life of the dense hydrogel in the myocardium was found to be long enough to prevent aneurysm rupture of the left ventricle wall, one of the more severe complications of the early post-infarction period

Viscosity Sensitive Dyes for Determining the Rheological Properties of Hydrogels in Biological Tissues

Tatarets A.L., Shkand T.V., Chizh M.O., Sleta I.V., Sandomirsky B.P., Patsenker L.D. Viscosity sensitive dyes for determining the rheological properties of hydrogels in biological tissues. The 14 th Conference on Methods and Applications in Fluorescence MAF 14 Würzburg, Germany. 13-16 September 2015. 232.Viscosity sensitive dyes for determining the rheological properties of hydrogels in biological tissue

Сравнительное изучение взаимодействия флуоресцентных красителей К-35 и К7-1045 с белками сыворотки крови крыс

Using the fluorescent spectroscopy, gel chromatography and electrophoresis in polyacrylamide gel methods interaction of fluorescent dyes K-35 and К7-1045 with rat serum proteins has been studied in the work in comparative aspect. It has been established that in serum K-35 and К7-1045 dyes bound mainly with albumin. It has been shown that K-35 dye may be used for estimating degree of rat serum albumin fullness by different ligands.В работе методами флуоресцентной спектроскопии, гель-хроматографии и электрофореза в полиакриламидном геле изучено в сравнительном аспекте взаимодействие флуоресцентных красителей К-35 и К7-1045 с белками сыворотки крови крыс. Установлено, что в сыворотке красители К-35 и К7-1045 главным образом связываются с альбумином. Показано, что краситель К-35 может быть применен для оценки степени нагруженности сывороточного альбумина крысы лигандами различного происхождения