Arginine methylation analysis of the splicing-associated SR protein SFRS9/SRP30C

The human SFRS9/SRp30c belongs to the SR family of splicing regulators. Despite evidence that members of this protein family may be targeted by arginine methylation, this has yet to be experimentally addressed. In this study, we found that SFRS9 is a target for PRMT1-mediated arginine methylation in vitro, and that it is immunoprecipitated from HEK-293 lysates by antibodies that recognize both mono- and dimethylated arginines. We further observed that upon treatment with the methylation inhibitor Adox, the fluorescent EGFP-SFRS9 re-localizes to dot-like structures in the cell nucleus. In subsequent confocal analyses, we found that EGFP-SFRS9 localizes to nucleoli in Adox-treated cells. Our findings indicate the importance of arginine methylation for the subnuclear localization of SFRS9144657669CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPsem informaçã

Recomendações para o cultivo do pomeleiro Flame nas condições do Vale do São Francisco.

O pomelo ou grapefruit (Citrus paradisiMacf.) é uma espécie cítrica considerada um híbrido natural de toranja, cuja origem presume-se ser da Jamaica ou Barbados. No Brasil é pouco conhecido, apresentando um sabor ácido e ligeiramente amargo, sendo utilizado na indústria de sucos, geleias, doces e cosméticos. Apresenta ainda propriedades medicinais relacionadas à perda de peso, ao controle da frequência cardíaca e da pressão arterial. Das espécies cítricas, o pomeleiro e a limeira ácida são as que têm se adaptado melhor à região Nordeste, principalmente no Submédio do Vale do São Francisco, região que apresenta características tipicamente tropicais e condições climáticas bastante .diferenciadasbitstream/item/175405/1/INT132-2.pd

Comportamento de pomeleiros e laranjeiras doce da Embrapa Mandioca e Fruticultura em relação ao Citrus Tristeza virus e ao descamamento eruptivo.

O Citrus tristeza virus (CTV) é uma das principais viroses que ameaçam as plantas cítricas no Brasil. O descamamento eruptivo dos citros (Bahia Bark Scaling, BBS) é uma doença restrita à citricultura da Bahia e de Sergipe, que afeta pomeleiros (C. paradisi Macfadyen), tangerineiras (C. reticulata Blanco) e laranjeiras doce (C. sinensis L.), e ainda tem sua etiologia e método diagnóstico não estabelecidos.PDF. 160_11

Spin-orbit implementation of Solovay-Kitaev decomposition of single-qubit channels

The Solovay-Kitaev theorem allows us to approximate any single-qubit gate to arbitrary accuracy with a finite sequence of fundamental operations from a universal set of gates. Inspired by this decomposition, we present a quantum channel simulator capable of implementing any completely positive trace-preserving map. Our realization consists of one ancillary qubit, encoded in the transverse mode of a laser beam (orbital degree of freedom), one qubit system, encoded in its polarization (spin), one spin-orbit CNOT gate and four single-qubit operations performed with prisms and polarization components. Our results describe the implementation of arbitrary single-qubit channels on the photon polarization using the transverse mode as the ancillary qubit.Comment: 13 pages, 15 figure

How do Candida glabrata´s biofilms respond to antifungal drugs?

Candida species are responsible for recurrent human infections, mostly in immunocompromised patients, due to their high vulnerability. Candida glabrata has been shown to have a major role in these infections being the second most prevalent species involved in human fungemia. Objective: To understand the effect of three different antifungal agents Fluconazole (Flu), Amphotericin B (AmB) and Caspofungin (Csf) - in C. glabratas biofilm formation, specially their role on matrix composition.Programa Operacional, Fatores de competitividade – COMPETE and by national funds through FCT – Fundação para a Ciência e a Tecnologia on the scope of the projects FCT PTDC/SAU-MIC/119069/2010, RECI/EBB- EBI/0179/2012, PEst-OE/EQB/LA0023/2013 Project “BioHealth - Biotechnology and Bioengineering approachesto improvehealthquality",Ref. NORTE-07-0124-FEDER-000027, co-funded by the Programa Operacional Regional do Norte (ON.2 – O Novo Norte), QREN, FEDE