9 research outputs found

    Comparasion between TiO2 thin films deposited by DC and RF sputtering

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    This paper compares titanium oxide (TiO2) thin films deposited by RF and DC sputtering. Structural characterization was used to investigate the morphology of TiO2 thin films. Both films show the rutile and anatase crystal structure; ellipsometry show thickness and refractive index of 50 nm and 2.43 for the TiO2 deposited by DC sputtering and 40 nm and 2.32 for the film by RF sputtering; AFM shows the roots mean square (RMS) roughness of 6.5 nm and 8 nm for TiO2 deposited by DC and RF sputtering, respectively. For electrical characterization was developed MOS capacitor; from them was possible to determine which method forms the best dielectric film, defined by high dielectric constant value (high-k), lower charge density (Q0/q) and flat-band voltage (VFB) around-0.9V. Therefore, the best method to deposit TiO2 is DC reactive sputtering; because this method showed a better electrical conditions and a well-defined crystalline structure.CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESSem informaçãoSem informação34. Symposium on Microelectronics Technology and Device

    Metagenomic Analysis Of A Tropical Composting Operation At The São Paulo Zoo Park Reveals Diversity Of Biomass Degradation Functions And Organisms

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    Composting operations are a rich source for prospection of biomass degradation enzymes. We have analyzed the microbiomes of two composting samples collected in a facility inside the São Paulo Zoo Park, in Brazil. All organic waste produced in the park is processed in this facility, at a rate of four tons/day. Total DNA was extracted and sequenced with Roche/454 technology, generating about 3 million reads per sample. To our knowledge this work is the first report of a composting whole-microbial community using high-throughput sequencing and analysis. The phylogenetic profiles of the two microbiomes analyzed are quite different, with a clear dominance of members of the Lactobacillus genus in one of them. We found a general agreement of the distribution of functional categories in the Zoo compost metagenomes compared with seven selected public metagenomes of biomass deconstruction environments, indicating the potential for different bacterial communities to provide alternative mechanisms for the same functional purposes. Our results indicate that biomass degradation in this composting process, including deconstruction of recalcitrant lignocellulose, is fully performed by bacterial enzymes, most likely by members of the Clostridiales and Actinomycetales orders. © 2013 Martins et al.84Ryckeboer, J., Mergaert, J., Vaes, K., Klammer, S., De Clercq, D., A survey of bacteria and fungi occurring during composting and self-heating processes (2003) Annals of Microbiology, 53, pp. 349-410Ishii, K., Takii, S., Comparison of microbial communities in four different composting processes as evaluated by denaturing gradient gel electrophoresis analysis (2003) Journal of Applied Microbiology, 95, pp. 109-119Steger, K., Eklind, Y., Olsson, J., Sundh, I., Microbial community growth and utilization of carbon constituents during thermophilic composting at different oxygen levels (2005) Microbial Ecology, 50, pp. 163-171Takebayashi, S., Narihiro, T., Fujii, Y., Hiraishi, A., Water availability is a critical determinant of a population shift from Proteobacteria to Actinobacteria during start-up operation of mesophilic fed-batch composting (2007) Microbes and Environments, 22, pp. 279-289Vargas-Garcia, M.C., Suarez-Estrella, F., Lopez, M.J., Moreno, J., Microbial population dynamics and enzyme activities in composting processes with different starting materials (2010) Waste Management, 30, pp. 771-778Partanen, P., Hultman, J., Paulin, L., Auvinen, P., Romantschuk, M., Bacterial diversity at different stages of the composting process (2010) BMC Microbiology, 10, p. 94Kumar, S., Composting of municipal solid waste (2011) Critical Reviews in Biotechnology, 31, pp. 112-136Peters, S., Koschinsky, S., Schwieger, F., Tebbe, C.C., Succession of microbial communities during hot composting as detected by PCR-single-strand-conformation polymorphism-based genetic profiles of small-subunit rRNA genes (2000) Applied and Environmental Microbiology, 66, pp. 930-936Alfreider, A., Peters, S., Tebbe, C.C., Rangger, A., Insam, H., Microbial community dynamics during composting of organic matter as determined by 16S ribosomal DNA analysis (2002) Compost Science & Utilization, 10, pp. 303-312Steger, K., Sjogren, A.M., Jarvis, A., Jansson, J.K., Sundh, I., Development of compost maturity and Actinobacteria populations during full-scale composting of organic household waste (2007) Journal of Applied Microbiology, 103, pp. 487-498Guo, Y., Zhu, N., Zhu, S., Deng, C., Molecular phylogenetic diversity of bacteria and its spatial distribution in composts (2007) Journal of Applied Microbiology, 103, pp. 1344-1354Franke-Whittle, I.H., Knapp, B.A., Fuchs, J., Kaufmann, R., Insam, H., Application of COMPOCHIP microarray to investigate the bacterial communities of different composts (2009) Microbial Ecology, 57, pp. 510-521Anastasi, A., Varese, G.C., Marchisio, V.F., Isolation and identification of fungal communities in compost and vermicompost (2005) Mycologia, 97, pp. 33-44Hultman, J., Vasara, T., Partanen, P., Kurola, J., Kontro, M.H., Determination of fungal succession during municipal solid waste composting using a cloning-based analysis (2010) Journal of Applied Microbiology, 108, pp. 472-487Bent, S.J., Forney, L.J., The tragedy of the uncommon: understanding limitations in the analysis of microbial diversity (2008) The ISME Journal, 2, pp. 689-695Hong, S.H., Bunge, J., Leslin, C., Jeon, S., Epstein, S.S., Polymerase chain reaction primers miss half of rRNA microbial diversity (2009) The ISME Journal, 3, pp. 1365-1373van Elsas, J.D., Boersma, F.G.H., A review of molecular methods to study the microbiota of soil and the mycosphere (2011) European Journal of Soil Biology, 47, pp. 77-87Gonzalez, J.M., Portillo, M.C., Belda-Ferre, P., Mira, A., Amplification by PCR Artificially Reduces the Proportion of the Rare Biosphere in Microbial Communities (2012) PloS ONE, 7, pp. e29973Lombard, N., Prestat, E., van Elsas, J.D., Simonet, P., Soil-specific limitations for access and analysis of soil microbial communities by metagenomics (2011) FEMS Microbiology Ecology, 78, pp. 31-49Allgaier, M., Reddy, A., Park, J.I., Ivanova, N., D'haeseleer, P., Targeted discovery of glycoside hydrolases from a switchgrass-adapted compost community (2010) PloS ONE, 5, pp. e8812Shokralla, S., Spall, J.L., Gibson, J.F., Hajibabaei, M., Next-generation sequencing technologies for environmental DNA research (2012) Molecular Ecology, 21, pp. 1794-1805Thomas, T., Gilbert, J., Meyer, F., Metagenomics - 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    Resposta diferencial de espécies e de híbridos de citros à leprose Differential response of citrus species and hybrids to leprosis

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    O objetivo deste trabalho foi buscar novas fontes de resistência à leprose-dos-citros, no Banco Ativo de Germoplasma do Centro APTA Citros Sylvio Moreira, Instituto Agronômico, em Cordeirópolis, SP. Foram utilizadas plantas obtidas por sementes de 26 acessos, infectadas com o vírus da leprose-dos-citros (Citrus leprosis virus - CiLV), por meio do seu vetor Brevipalpus phoenicis. O aparecimento de lesões, a partir de 21 dias após a inoculação, foi observado em 11 dos genótipos testados (42,3%). Quinze espécies, entre elas Citrus pennivesiculata e C. celebica, comportaram-se como altamente resistentes, enquanto outras, como C. keraji, foram mais suscetíveis que o padrão C. sinensis. Os dados mostraram grande variação de respostas de Citrus spp. à leprose, com elevado número de espécies resisentes, que podem ser utilizadas como fonte de resistência à doença em programas de melhoramento.<br>The objective of this work was to prospect for new sources of resistance to leprosis in the Citrus Germplasm Bank of Centro APTA Citros Sylvio Moreira, Instituto Agronômico, in Cordeirópolis, SP, Brazil. Plants obtained through seeds from 26 citrus accessions were mite-infected with Citrus leprosis virus (CiLV). Typical lesions started to appear in 11 genotypes (42.3% of the tested accessions) 21 days after inoculation. Fifteen species, among them Citrus pennivesiculata and C. celebica, behaved as highly resistant, while other genotypes, as C. keraji, were more susceptible than the standard C. sinensis. The data showed a wide variation in the response of Citrus spp. to leprosis, with a large number of resistant species, wich can be used as source of resistance to this disease, in breeding programs

    The citrus leprosis pathosystem O patossistema leprose dos citros

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    Citrus leprosis is considered the main viral disease for the Brazilian citrus production, particularly for the State of São Paulo, due to the high costs spent for the chemical control of its vector, the tenuipalpid mite Brevipalpus phoenicis. In addition, its global importance has significantly increased in the last years, with the dissemination of the virus to new countries in South and Central America. In Brazil, despite its economical importance and occurrence for more than seven decades, the most significant advances towards understanding the pathosystem interactions have been obtained only in the last ten years. This review focuses on various aspects of the disease, beginning with a historical view, its main characteristics, alternatives for its control, its increasing economical importance in Brazil and abroad, and the new data on the search for understanding the interactions amongst the mite vector, the virus, and the plant host.<br>A leprose dos citros é considerada a principal virose na citricultura brasileira, com maior destaque no Estado de São Paulo, principalmente pelos altos custos demandados para o controle químico do vetor, o ácaro Brevipalpus phoenicis. Além da relevância dessa virose para a citricultura local, sua importância mundial vem sendo ampliada consideravelmente nos últimos anos, principalmente com a disseminação do vírus em novos países da América do Sul e Central. No Brasil, apesar da sua importância econômica e ocorrência por mais de sete décadas, os mais importantes avanços no entendimento das interações do patossistema leprose têm sido obtidos apenas nos últimos dez anos. Essa revisão aborda os diferentes aspectos dessa doença, trazendo um breve histórico da doença, principais características da leprose, alternativas de controle, sua crescente importância econômica na cadeia citrícola nacional, os mais recentes relatos de sua ocorrência em outros países e os novos resultados obtidos pela pesquisa buscando um melhor entendimento das interações entre ácaro, vírus e hospedeiro vegetal
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