Effects of Inoculation of LAB on Fermentation Pattern and Clostridia Spores in Easily Ensilable Grass Silages

Clostridia can damage the protein quality of grass silages. They cause high gas losses during the fermentation process and quality problems in dairy products like semi-hard cheeses. In comparison to the effect of chemicals such as nitrite on undesirable clostridia in grass silages the respective inhibitory mechanism of LAB requires further investigation. The objective of this experiment was to study under laboratory conditions novel isolates of lactic acid bacteria (LAB), selected for their inhibitory effect on clostridia in grass silages

The Bouguer-Beer-Lambert Law: Shining Light on the Obscure

The Beer-Lambert law is unquestionably the most important law in optical spectroscopy and indispensable for the qualitative and quantitative interpretation of spectroscopic data. As such, every spectroscopist should know its limits and potential pitfalls, arising from its application, by heart. It is the goal of this work to review these limits and pitfalls, as well as to provide solutions and explanations to guide the reader. This guidance will allow a deeper understanding of spectral features, which cannot be explained by the Beer-Lambert law, because they arise from electromagnetic effects/the wave nature of light. Those features include band shifts and intensity changes based exclusively upon optical conditions, i. e. the method chosen to record the spectra, the substrate and the form of the sample. As such, the review will be an essential tool towards a full understanding of optical spectra and their quantitative interpretation based not only on oscillator positions, but also on their strengths and damping constants

An \u3cem\u3ein vitro\u3c/em\u3e Study on the Influence of Residual Sugars on Aerobic Changes in Grass Silages

How do residual sugars in high dry matter grass silages influence microbial metabolism? To answer this question a simple laboratory method was developed using pH as main indicator for aerobic changes

A Comparison of the Efficacy of an Ultra-Low Volume Applicator for Liquid-Applied Silage Inoculants with That of a Conventional Applicator

Liquid-applied silage inoculants are normally sprayed onto forages cut for ensiling at application rates from 1 to 3 l/t. Applicator tanks can require frequent re-filling, especially with large self-propelled forage harvesters having harvest rates in excess of 1000 t/d. This can be an issue for fields remote from the farm, for areas with restricted water availability and for contractors paid by the area harvested. This study was conducted to assess the efficacy of inoculant distribution on the crop using a simple, ultra-low volume (ULV) applicator compared with a conventional liquid-applied silage inoculant applicator

Perennial Ryegrasses Bred for Contrasting Sugar Contents: Manipulating Fermentation and Aerobic Stability of Wilted Silage Using Additives (3) (EU-Project ‘SweetGrass’)

Rapid field-drying of grass prior to successful ensilage restricts fermentation and can assist preservation, but can consequently result in silages that are prone to aerobic deterioration at feedout. Additives that directly (e.g. potassium sorbate or sodium benzoate) or indirectly (e.g. formic acid or Lactobacillus plantarum, via manipulation of fermentation) alter yeast activity at feedout could modify silage aerobic stability. This experiment evaluated the fermentation and aerobic stability of wilted silages made from perennial ryegrass cultivars of high or normal water soluble carbohydrate (WSC) genotype that differed in additive use

Perennial Ryegrasses Bred for Contrasting Sugar Contents: Manipulating Fermentation and Aerobic Stability of Unwilted Silage using Additives (2) (EU-Project ‘SweetGrass’)

Grass cultivars bred for elevated concentrations of water-soluble carbohydrate (WSC) could have improved silage preservation but possibly disimproved aerobic stability. Additives can be used to manipulate fermentation and thereby increase silage WSC. They can also influence aerobic stability. This experiment evaluated the fermentation and aerobic stability of unwilted silages made from perennial ryegrass cultivars of high or normal WSC genotype that differed in additive use

Expression of mRNA of the col2a1b gene during zebrafish embryogenesis

We set out to characterize the expression of col2a1b, the zebrafish ortholog of the human COL2A1. In zebrafish, the col2a1a gene has been shown to be expressed in both chondrocytes and perichondrial cells, whereas our preliminary work suggests col2a1b is expressed only in the perichondrium of cartilage elements; thus, col2a1b collagen promotes cartilage development and ossification of chondrocytes. Our lab is interested in understanding the differential expression of the two homologs. In this report, we document the complete spatiotemporal expression of the zebrafish col2a1b mRNA during the first 5 days of development via in situ hybridization

Effect of Residual Sugar in High Sugar Grass Silages on Aerobic Stability

New varieties of Lolium perenne, bred for high sugar content, can contain up to 30% of water soluble carbohydrates (WSC). Only a fraction of such high contents are metabolised during a normal fermentation and the high residual sugar content (RSC) of these silages can improve the efficiency of use of nitrogen by ruminants. However, these RSC at opening for feed-out could be preferentially metabolised relative to fermentation products by all aerobically growing fungi and bacteria present on the forage. A high RSC thus can increase the risk of aerobic deterioration over that of extensively fermented silages, containing predominantly organic acids, which are initially utilised by certain yeasts. The objective of this study was to assess the relationship between RSC and aerobic stability of silages prepared with either optimal ensiling conditions or with a defined air challenge treatment to make them prone to aerobic deterioration. The latter is a useful method to test the efficacy of aerobic stability improving silage additives, requiring unstable controls (Pahlow et al., 1999)

Perennial Ryegrasses Bred for Contrasting Sugar Contents: Manipulating Fermentation and Aerobic Stability Using Wilting and Additives (1) (EU FP V -Project ‘SweetGrass’)

Higher concentrations of water-soluble carbohydrate (WSC) in silage offer ruminant nutrition and environmental attractions. Both successful field wilting and alternative silage additives provide the opportunity to manipulate silage WSC by modifying fermentation and/or improving aerobic stability. This experiment evaluated the fermentation and aerobic stability of silages made from perennial ryegrass cultivars of high or normal WSC genotype that differed in field wilting or additive use

Revisiting the Local Scaling Hypothesis in Stably Stratified Atmospheric Boundary Layer Turbulence: an Integration of Field and Laboratory Measurements with Large-eddy Simulations

The `local scaling' hypothesis, first introduced by Nieuwstadt two decades ago, describes the turbulence structure of stable boundary layers in a very succinct way and is an integral part of numerous local closure-based numerical weather prediction models. However, the validity of this hypothesis under very stable conditions is a subject of on-going debate. In this work, we attempt to address this controversial issue by performing extensive analyses of turbulence data from several field campaigns, wind-tunnel experiments and large-eddy simulations. Wide range of stabilities, diverse field conditions and a comprehensive set of turbulence statistics make this study distinct