7th Drug hypersensitivity meeting: part two

No abstract availabl

Vicilins (7S storage globulins) of cowpea (Vigna unguiculata) seeds bind to chitinous structures of the midgut of Callosobruchus maculatus (Coleoptera: Bruchidae) larvae

The presence of chitin in midgut structures of Callosobruchus maculatus larvae was shown by chemical and immunocytochemical methods. Detection by Western blotting of cowpea (Vigna unguiculata) seed vicilins (7S storage proteins) bound to these structures suggested that C. maculatus-susceptible vicilins presented less staining when compared to C. maculatus-resistant vicilins. Storage proteins present in the microvilli in the larval midgut of the bruchid were recognized by immunolabeling of vicilins in the appropriate sections with immunogold conjugates. These labeling sites coincided with the sites labeled by an anti-chitin antibody. These results, taken together with those previously published showing that the lower rates of hydrolysis of variant vicilins from C. maculatus-resistant seeds by the insect's midgut proteinases and those showing that vicilins bind to chitin matrices, may explain the detrimental effects of variant vicilins on the development of C. maculatus larvae

Population Genetic Structure of the Threatened Amazon River Turtle, Podocnemis sextuberculata (Testudines, Podocnemididae)

Podocnemis sextuberculata (Pleurodira: Podocnemididae) is widely distributed throughout the Amazon drainage basin in Brazil, Colombia, and Peru. Telemetry and previous molecular data suggest that P. sextuberculata lacks population structure in the central Amazon basin of Brazil. Generalization of these results, however, requires much-broader sampling across a range of habitats of this broadly distributed species. For this reason, we tested the hypothesis of panmixia in P. sextuberculata, analyzing a total of 319 specimens sequenced for the mitochondrial control region. Our sampling included localities from 16 areas in the Amazon basin from rivers characteristic of the Amazon basin (whitewater), clearwater rivers of the Guiana shield (Branco, Trombetas, and Nhamundá rivers), and the Brazilian shield (Xingu River). The hypothesis of panmixia was rejected because the results of analysis of molecular variance, pairwise ST, and Bayesian analysis of population structure indicated population structure in the group of individuals from the locality of Xingu which was not correlated to a pattern of isolation by distance. We suggest that the populations of P. sextuberculata of the Brazilian Amazon basin are composed of 2 management units, one represented by populations restricted to the Xingu River and the other represented by all other populations. The population of the Xingu should be viewed with attention and concern, especially considering the direct and indirect impacts of damming the Xingu River. © 2017 Chelonian Research Foundation

Dengue

Lecture 49 ISBN e-book : 9781615045754International audienc