Use of Molecular Genetic Engineering in the Study of Animal Parasites and Their Vectors

Molecular genetics coupled with advances in immunology and parasite culture has become a powerful tool to study animal parasites and their vectors. Recombinant DNA techniques allow one to identify individual genes of DNA probes, amplify the nucleic acid of interest, and use this material to study: the role of the gene product in the biology of the organism; the evolution of parasites and their hosts; heterogeneity between species and within species; taxonomy and develop refined taxonomic tools; the immunology and biochemistry of host-parasite interactions; identification of specific cells or tissues that produce gene products; cytogenetics and localization of genes on chromosome in the study of animal parasites and their vectors will be presented

Molecular Approach to the Study of Trematode Parasites : the Blood Fluke

One important aspect of reproductive development in trematode parasites is the formation of a hardened eggshell which allows the zygote to develop into a miracidium in a hostile environment. The miracidium then can transfer the germline from the vertebrate host to snail intermediate host. Schistosome parasites, unlike other trematodes, have separate sexes and female reproductive development is known to depend on the presence of a male parasite. These facts make the blood flukes ideal material to study the mechanisms that underlie female reproductive development and eggshell formatian. We reasoned that the morphological and biochemical differences between the male and female must be reflected at the molecular level in the differential expression of sexually regulated genes. Radioactive single stranded cDNA was first transcribed from female RNA; and then sequences common to both male and female were removed by hybridization to an excess of male RNA. This probe was used to screen a cDNA library made from mRNA of adult worm paris. One hybridizing clone, pSMf 61-46, was shown to correspond to a 0.9 kilobase mRNA that is present only in mature female worms and is not detectable in female schistosomes from single-sex infections, in male worms or in eggs. Thus expression of the gene was female-specific. During normal bisexual infection this mRNA is first detected 28 days after infection (the time of worm pairing) and increases to a high level at 35 days postinfection, coinciding with egg production. Thus the temporal expression of the gene was dependent on paining with male worm. The nucleotide sequence of the gene shows an open reading frame that encodes a 16 kDA polypeptide that shows strong homology with eggshell proteins on insects. A second female-specific cDNA clone, F-4, represents a 1.6 kilobase mRNA whose expression is also correlated with worm pairing and subsequent egg production, encodes a different putative eggshell component of 44 kDA. The amino acid composition of the 16 kDA and 44 kDA polypeptides show a strong correlation with the actual amino acid composition of the schistosome eggshell. Thus these two polypeptides appear to the major components of the schistosome eggshell. Analysis of the genomic arrangement of the eggshell genes show that pl6 is represented by 5 gene copies and p48 is represented by 2-5 copies. The eggshell genes are expressed in the vitelline cell as recently demonstrated by in hybridization and immunocytochemical localization. The eggshell genes are being expressed in bacteria. The gene products will be used to study the biochemistry of eggshell formation

Scanning electron microscope observations on the miracidium of Schistosoma

Miracidia of two species of Schistosoma, viz. haematobium and japonicum, were studied with the scanning electron microscope to more clearly visualize what is seen with the light microscope and the transmission electron microscope, and more specifically, to relate the structure of the apical papilla to its function in snail penetration.The apical papilla of schistosome miracidia is composed of corrugated areas which form tiny suckerlike cups, presumably used by the miracidium to facilitate attachment to the snail during penetration. A lateral opening (secretory pore) on the apical papilla and short stubby apical cilia (tactile or sensory) are also demonstrated.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/22128/1/0000555.pd

Gravitational Lensing as Signal and Noise in Lyman-alpha Forest Measurements

In Lyman-alpha forest measurements it is generally assumed that quasars are mere background light sources which are uncorrelated with the forest. Gravitational lensing of the quasars violates this assumption. This effect leads to a measurement bias, but more interestingly it provides a valuable signal. The lensing signal can be extracted by correlating quasar magnitudes with the flux power spectrum and with the flux decrement. These correlations will be challenging to measure but their detection provides a direct measure of how features in the Lyman-alpha forest trace the underlying mass density field. Observing them will test the fundamental hypothesis that fluctuations in the forest are predominantly driven by fluctuations in mass, rather than in the ionizing background, helium reionization or winds. We discuss ways to disentangle the lensing signal from other sources of such correlations, including dust, continuum and background residuals. The lensing-induced measurement bias arises from sample selection: one preferentially collects spectra of magnified quasars which are behind overdense regions. This measurement bias is ~0.1-1% for the flux power spectrum, optical depth and the flux probability distribution. Since the effect is systematic, quantities such as the amplitude of the flux power spectrum averaged across scales should be interpreted with care.Comment: 22 pages, 8 figures; v2: references added, discussion expanded, matches PRD accepted versio

Rapidly-Varying Speed of Sound, Scale Invariance and Non-Gaussian Signatures

We show that curvature perturbations acquire a scale invariant spectrum for any constant equation of state, provided the fluid has a suitably time-dependent sound speed. In order for modes to exit the physical horizon, and in order to solve the usual problems of standard big bang cosmology, we argue that the only allowed possibilities are inflationary (albeit not necessarily slow-roll) expansion or ekpyrotic contraction. Non-Gaussianities offer many distinguish features. As usual with a small sound speed, non-Gaussianity can be relatively large, around current sensitivity levels. For DBI-like lagrangians, the amplitude is negative in the inflationary branch, and can be either negative or positive in the ekpyrotic branch. Unlike the power spectrum, the three-point amplitude displays a large tilt that, in the expanding case, peaks on smallest scales. While the shape is predominantly of the equilateral type in the inflationary branch, as in DBI inflation, it is of the local form in the ekpyrotic branch. The tensor spectrum is also generically far from scale invariant. In the contracting case, for instance, tensors are strongly blue tilted, resulting in an unmeasurably small gravity wave amplitude on cosmic microwave background scales.Comment: 41 pages, 12 figures. v4: Few typos in equations (7.39) correcte

Scale-dependence of Non-Gaussianity in the Curvaton Model

We investigate the scale-dependence of f_NL in the self-interacting curvaton model. We show that the scale-dependence, encoded in the spectral index n_{f_NL}, can be observable by future cosmic microwave background observations, such as CMBpol, in a significant part of the parameter space of the model. We point out that together with information about the trispectrum g_NL, the self-interacting curvaton model parameters could be completely fixed by observations. We also discuss the scale-dependence of g_NL and its implications for the curvaton model, arguing that it could provide a complementary probe in cases where the theoretical value of n_{f_NL} is below observational sensitivity.Comment: 14 pages, 5 figures, Eq.(10) correcte

Effects and Detectability of Quasi-Single Field Inflation in the Large-Scale Structure and Cosmic Microwave Background

Quasi-single field inflation predicts a peculiar momentum dependence in the squeezed limit of the primordial bispectrum which smoothly interpolates between the local and equilateral models. This dependence is directly related to the mass of the isocurvatons in the theory which is determined by the supersymmetry. Therefore, in the event of detection of a non-zero primordial bispectrum, additional constraints on the parameter controlling the momentum-dependence in the squeezed limit becomes an important question. We explore the effects of these non-Gaussian initial conditions on large-scale structure and the cosmic microwave background, with particular attention to the galaxy power spectrum at large scales and scale-dependence corrections to galaxy bias. We determine the simultaneous constraints on the two parameters describing the QSF bispectrum that we can expect from upcoming large-scale structure and cosmic microwave background observations. We find that for relatively large values of the non-Gaussian amplitude parameters, but still well within current uncertainties, galaxy power spectrum measurements will be able to distinguish the QSF scenario from the predictions of the local model. A CMB likelihood analysis, as well as Fisher matrix analysis, shows that there is also a range of parameter values for which Planck data may be able distinguish between QSF models and the related local and equilateral shapes. Given the different observational weightings of the CMB and LSS results, degeneracies can be significantly reduced in a joint analysis.Comment: 27 pages, 14 figure

Primordial non-Gaussianity in the Bispectrum of the Halo Density Field

The bispectrum vanishes for linear Gaussian fields and is thus a sensitive probe of non-linearities and non-Gaussianities in the cosmic density field. Hence, a detection of the bispectrum in the halo density field would enable tight constraints on non-Gaussian processes in the early Universe and allow inference of the dynamics driving inflation. We present a tree level derivation of the halo bispectrum arising from non-linear clustering, non-linear biasing and primordial non-Gaussianity. A diagrammatic description is developed to provide an intuitive understanding of the contributing terms and their dependence on scale, shape and the non-Gaussianity parameter fNL. We compute the terms based on a multivariate bias expansion and the peak-background split method and show that non-Gaussian modifications to the bias parameters lead to amplifications of the tree level bispectrum that were ignored in previous studies. Our results are in a good agreement with published simulation measurements of the halo bispectrum. Finally, we estimate the expected signal to noise on fNL and show that the constraint obtainable from the bispectrum analysis significantly exceeds the one obtainable from the power spectrum analysis.Comment: 34 pages, 15 figures, (v3): matches JCAP published versio

Effect of human TGF-beta on the gene expression profile of Schistosoma mansoni adult worms

Schistosoma mansoni is responsible for schistosomiasis, a parasitic disease that affects 200 million people worldwide. Molecular mechanisms of host-parasite interaction are complex and involve a crosstalk between host signals and parasite receptors. TGF-beta signaling pathway has been shown to play an important role in S. mansoni development and embryogenesis. In particular human (h) TGF-beta has been shown to bind to a S. mansoni receptor, transduce a signal that regulates the expression of a schistosome target gene. Here we describe 381 parasite genes whose expression levels are affected by in vitro treatment with hTGF-beta. Among these differentially expressed genes we highlight genes related to morphology, development and cell cycle that could be players of cytokine effects on the parasite. We confirm by qPCR the expression changes detected with microarrays for 5 out of 7 selected genes. We also highlight a set of non-coding RNAs transcribed from the same loci of protein-coding genes that are differentially expressed upon hTCF-beta treatment. These datasets offer potential targets to be explored in order to understand the molecular mechanisms behind the possible role of hTGF-beta effects on parasite biology. (C) 2012 Elsevier B.V. All rights reserved.Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)NIH [AI 46762]NIHCNPqCNPqNIAIDNIAID [HHSN272201000009I

Extracting the late-time kinetic Sunyaev-Zel'dovich effect

We propose a novel technique to separate the late-time, post-reionization component of the kinetic Sunyaev Zeldovich (kSZ) effect from the contribution to it from a (poorly understood and probably patchy) reionization history. The kSZ effect is one of the most promising probe of the missing baryons in the Universe. We study the possibility of reconstructing it in three dimensions (3D), using future spectroscopic surveys such as the Euclid survey. By reconstructing a 3D template from galaxy density and peculiar velocity fields from spectroscopic surveys we cross-correlate the estimator against CMB maps. The resulting cross-correlation can help us to map out the kSZ contribution to CMB in 3D as a function of redshift thereby extending previous results which use tomographic reconstruction. This allows the separation of the late-time effect from the contribution owing to reionization. By construction, it avoids contamination from foregrounds, primary CMB, tSZ effect as well as from star-forming galaxies. Due to a high number density of galaxies the signal-to-noise ratio (S/N) for such cross-correlational studies is higher, compared to the studies involving CMB power-spectrum analysis. Using a spherical Bessel–Fourier (sFB) transform we introduce a pair of 3D power spectra: C⊥ℓ(k) and C⊥ℓ(k) that can be used for this purpose. We find that in a future spectroscopic survey with near all-sky coverage and a survey depth of z ≈ 1, reconstruction of C⊥ℓ(k) can be achieved in a few radial wave bands k ≈ (0.01–0.5 h−1 Mpc) with a S/N ratio of up to O(10) for angular harmonics in the range = (200–2000)