10 research outputs found
Bicyclo[4.2.0]octa-1,3,5-trien-3-yl-dimethyl((E)-styryl)-silane
Bicyclo[4.2.0]octa-1,3,5-trien-3-yl-dimethyl-((E)-styryl)-silane was synthesized via three stage synthesis starting from benzocyclobutene and (2-bromo-vinyl)-benzene. The structure of the product was determined using 1H- and 13C-NMR and HRMS
Di(bicyclo[4.2.0]octa-1(6),2,4-trien-3-yl)dimethylsilane
Di(bicyclo[4.2.0]octa-1(6),2,4-trien-3-yl)dimethylsilane (DiBCB-DMS) was synthesized as a promising monomer for polymer coating production with good dielectric properties for electronics. DiBCB-DMS was characterized by 1H, 13C NMR, IR, UV/VIS, and HRMS analysis
Quantum Dot Loaded Liposomes As Fluorescent Labels for Immunoassay
Liposomes loaded with water-soluble
and water-insoluble quantum
dots (QD) were for the first time applied as labels in different heterogeneous
immunoassays for the determination of food contaminants, using mycotoxin
zearalenone (ZEN) as a model. A great deal of work was devoted to
the optimal choice of phospholipids for the liposomes preparation
and to the factors which are important for the stability and size
of obtained liposomes. Thin-film hydration and reverse-phase evaporation
techniques were evaluated in terms of stability of the obtained liposomes
and their efficiency for QD loading. Conjugation of liposomes with
proteins and the influence of cross-linkers to the nonspecific interaction
of the obtained liposomes with the surface of microtiter plates and
cartridges were investigated and 3-(2-pyridyldithio)Âpropionic acid <i>N</i>-hydroxysuccinimide ester was found as the optimal cross-linker.
The limits of detection (LOD) for ZEN of fluorescence-labeled immunosorbent
assays were 0.6 μg kg<sup>–1</sup>, 0.08 μg kg<sup>–1</sup>, and 0.02 μg kg<sup>–1</sup>, using
QD, liposomes loaded with water-soluble QD, and water-insoluble QD,
respectively. Similarly, the developed qualitative on-site tests using
the different QD labels and taking into account the EU maximum residues
level for ZEN in unprocessed cereals showed cutoff levels of 100,
50, and 20 μg kg<sup>–1</sup>