Large Deviations in Weakly Interacting Fermions - Generating Functions as Gaussian Berezin Integrals and Bounds on Large Pfaffians

We prove that the G\"{a}rtner--Ellis generating function of probability distributions associated with KMS states of weakly interacting fermions on the lattice can be written as the limit of logarithms of Gaussian Berezin integrals. The covariances of the Gaussian integrals are shown to have a uniform Pfaffian bound and to be summable in general cases of interest, including systems that are \emph{not} translation invariant. The Berezin integral representation can thus be used to obtain convergent expansions of the generating function in terms of powers of its parameter. The derivation and analysis of the expansions of logarithms of Berezin integrals are the subject of the second part of the present work. Such technical results are also useful, for instance, in the context of quantum information theory, in the computation of relative entropy densities associated with fermionic Gibbs states, and in the theory of quantum normal fluctuations for weakly interacting fermion systems.FAPESP (2017/22340-9) CNPq (309723/2020-5) by the Basque Government through the grant IT641-13 MTM2017-82160-C2-2-P

Gene silencing in tick cell lines using small interfering or long double-stranded RNA

Gene silencing by RNA interference (RNAi) is an important research tool in many areas of biology. To effectively harness the power of this technique in order to explore tick functional genomics and tick-microorganism interactions, optimised parameters for RNAi-mediated gene silencing in tick cells need to be established. Ten cell lines from four economically important ixodid tick genera (Amblyomma, Hyalomma, Ixodes and Rhipicephalus including the sub-species Boophilus) were used to examine key parameters including small interfering RNA (siRNA), double stranded RNA (dsRNA), transfection reagent and incubation time for silencing virus reporter and endogenous tick genes. Transfection reagents were essential for the uptake of siRNA whereas long dsRNA alone was taken up by most tick cell lines. Significant virus reporter protein knockdown was achieved using either siRNA or dsRNA in all the cell lines tested. Optimum conditions varied according to the cell line. Consistency between replicates and duration of incubation with dsRNA were addressed for two Ixodes scapularis cell lines; IDE8 supported more consistent and effective silencing of the endogenous gene subolesin than ISE6, and highly significant knockdown of the endogenous gene 2I1F6 in IDE8 cells was achieved within 48 h incubation with dsRNA. In summary, this study shows that gene silencing by RNAi in tick cell lines is generally more efficient with dsRNA than with siRNA but results vary between cell lines and optimal parameters need to be determined for each experimental system

Ecological restoration in mediterranean-type shrublands and woodlands

The Mediterranean-type ecosystems (further MTEs) are limited to five regions on Earth (Cowling et al. 1996): Mediterranean Basin, California, Central Chile, the Cape of South Africa and the Southwest (and partly South) Australia. These regions are characteristic of western ocean coastlines in warm-temperate latitudes characterized by descending water-deprived ethesial winds. They are invariably transitional between temperate forests and semi-deserts. Precipitation seasonality and prevalence of winter-rainfall/summer drought cycling are regular, although not exclusive to the MTEs (Blumler 2005; Rebelo et al. 2006). Fire has been part of the natural regeneration cycles and undoubtedly also evolutionary history of the scrublands and woodlands (perhaps except for the Chilean MTE) for millions of years. The Northern Hemisphere MTEs and the Central Chilean MTE are home to relatively young geologically and climatically dynamic landscapes. The MTEs of the African Cape and Australia are, on the other hand, geologically quiescent and climatically buffered – most of these regions qualify as Old Stable Landscapes (Hopper 2009; Mucina and Wardell-Johnson 2011). MTEs are evolutionary hotbeds and musea: they are home to several global centres of biodiversity (Myers et al. 2000) and have about 20 per cent of total floristic diversity in an area covering just 5 per cent of the land surface. Vegetation of the MTEs is typically sclerophyllous shrublands, however (pine, oak, eucalyptus) woodlands are also important. Besides the enormous biodiversity, the regions supporting MTEs have been under human pressure for a long time. Some (Mediterranean Basin and its eastern outposts in the Middle East) have been the cradle of agriculture and have seen the rise of many civilizations. Past and present human use put these ecosystems under pressure and where possible and feasible, restoration of these ecosystems emerged as one of the ways for their wise, future-oriented management. Each of the partial MTEs is exposed to multiple challenges of rehabilitation and a profound review of these is beyond the scope of this chapter. Therefore we have embarked on featuring the dominant rehabilitation focus in each MTE

European position paper on the management of patients with patent foramen ovale. General approach and left circulation thromboembolism

The presence of a patent foramen ovale (PFO) is implicated in the pathogenesis of a number of medical conditions; however, the subject remains controversial and no official statements have been published. This interdisciplinary paper, prepared with involvement of eight European scientific societies, aims to review the available trial evidence and to define the principles needed to guide decision making in patients with PFO. In order to guarantee a strict process, position statements were developed with the use of a modified grading of recommendations assessment, development, and evaluation (GRADE) methodology. A critical qualitative and quantitative evaluation of diagnostic and therapeutic procedures was performed, including assessment of the risk/benefit ratio. The level of evidence and the strength of the position statements of particular management options were weighed and graded according to predefined scales. Despite being based often on limited and non-randomised data, while waiting for more conclusive evidence, it was possible to conclude on a number of position statements regarding a rational general approach to PFO management and to specific considerations regarding left circulation thromboembolism. For some therapeutic aspects, it was possible to express stricter position statements based on randomised trials. This position paper provides the first largely shared, interdisciplinary approach for a rational PFO management based on the best available evidence

Intermediate follow-up following intravascular stenting for treatment of coarctation of the aorta

Background : We report a multiinstitutional study on intermediate-term outcome of intravascular stenting for treatment of coarctation of the aorta using integrated arch imaging (IAI) techniques. Methods and Results : Medical records of 578 patients from 17 institutions were reviewed. A total of 588 procedures were performed between May 1989 and Aug 2005. About 27% (160/588) procedures were followed up by further IAI of their aorta (MRI/CT/repeat cardiac catheterization) after initial stent procedures. Abnormal imaging studies included: the presence of dissection or aneurysm formation, stent fracture, or the presence of reobstruction within the stent (instent restenosis or significant intimal build-up within the stent). Forty-one abnormal imaging studies were reported in the intermediate follow-up at median 12 months (0.5–92 months). Smaller postintervention of the aorta (CoA) diameter and an increased persistent systolic pressure gradient were associated with encountering abnormal follow-up imaging studies. Aortic wall abnormalities included dissections ( n = 5) and aneurysm ( n = 13). The risk of encountering aortic wall abnormalities increased with larger percent increase in CoA diameter poststent implant, increasing balloon/coarc ratio, and performing prestent angioplasty. Stent restenosis was observed in 5/6 parts encountering stent fracture and neointimal buildup ( n = 16). Small CoA diameter poststent implant and increased poststent residual pressure gradient increased the likelihood of encountering instent restenosis at intermediate follow-up. Conclusions : Abnormalities were observed at intermediate follow-up following IS placement for treatment of native and recurrent coarctation of the aorta. Not exceeding a balloon:coarctation ratio of 3.5 and avoidance of prestent angioplasty decreased the likelihood of encountering an abnormal follow-up imaging study in patients undergoing intravascular stent placement for the treatment of coarctation of the aorta. We recommend IAI for all patients undergoing IS placement for treatment of CoA. © 2007 Wiley-Liss, Inc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/57392/1/21191_ftp.pd