Selective mobilization of fatty acids in adipose tissue of heavy pigs

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Livres e iguais: A (in)eficacia do principio da dignidade humana para salvaguardar os direitos das pessoas trans

Em virtude do contexto de imposição de uma ordemdiscursiva cisheteronormativa compulsória, que determina acoerência e continuidade entre sexo-gênero-sexualidade para sustentar a normatização da vida dos indivíduos, aqueles que não se classificam na bipolaridade (homem/mulher) ficam à margem da própria sociedade. E é nesse sentido que se ressalta a importância da abordagem de gênero. Nesse ínterim, através do estudo do método dedutivo-bibliográfico, o presente trabalho pretende analisar as experiências identitárias da transexualidade e da travestilidade. Isso se dará através dos estudos de Judith Butler, que afirma que o gênero é concebido a partir de ordens discursivas que se traçam com base nas relações de poder, o que influencia na determinação de um mundo binário, por meio de técnicas da normalização, que acaba limitando os espaços de constituição identitária dos sujeitos humanos. Dessa forma, a experiência trans, ao ser uma experiência identitária transgressora das normas de gênero e também em virtude da precariedade social, ao desvirtuar tais normas, relega a travestis e transexuais o status de abjeção, sendo socialmente marginalizados e considerados como ininteligível, o que lhes resulta em uma vulnerabilidade à violências físicas e simbólicas, dando ensejo a violações de direitos humanos que podem ganhar contornos radicais como a negação do direito a vida. Nesse sentido, o presente trabalho pretende fazer uma breve análise sobre se os direitos humanos, simbolizados principalmente pelo princípio da dignidade humana, são aplicados para salvaguardar os direitos dos indivíduos trans, buscando compreender como alguns organismos internacionais de proteção de direitos humano

Desenvolvimento humano e bioético: concretizando princípios de direitos humanos

Este trabalho busca uma reflexão abordando o surgimento e o papel da bioética enquanto estratégia de garantia de Direitos Humanos face aos horrores da guerra e as experimentações científicas com humanos ao longo do último século e não apenas como instrumento utilizado para estabilizar ou regular as relações médico-paciente. Tem-se como proposta a reflexão, quanto à imposição de limites com a introdução dos princípios da autonomia, beneficência, não maleficência e justiça e, se reforçam a construção principiológica voltada para a realidade da vulnerabilidade humana, considerando a frequência e rotina em que Direitos Humanos são violados, a partir dessa construção contemporânea que temos de Direito. Observam-se violações regulares e frequentes a direitos básicos quando, esses grupos em condição de vulnerabilidade, nas suas variadas dimensões, não apenas econômicas, deixam de ter acesso à saúde ou às novas tecnologias introduzidas pelas pesquisas

Effect of feeding maltodextrins and dextrose on rearing and slaughtering performance of immunocastrated male pigs

This study aimed to verify previous research findings showing that feeding maltodextrins (M) and dextrose (D) leads to a reduction of voluntary feed intake in the Italian heavy pig. This may be interesting for immunocastrated pigs fed ad libitum (ad lib.), whose feed intake skyrockets after the 2nd vaccination, causing too fatty carcasses at slaughtering. Thirty-six male pigs (Italian Duroc x Italian Large White crossbred) received a double immunocastrating injection at 90 and 162 days of age. At 120 days, weighing 51.84+4.38 kg, the subjects were evenly housed in 9 boxes, fed ad lib. till the 2nd injection and then given, until slaughtering (197 days of age; 144.51±9.70 kg), one of the experimental diets: control diet, ad lib. (CL); control diet, restricted at 7.5% l.w.0.75 (CR); with MD (3.5 + 3.5%; adjusted for energy and protein), ad lib. (MD). The CR diet was introduced as the alternative choice to avoid too fatty carcasses. Compared to the restricted ones, pigs fed ad lib. (with or without MD) showed statistically higher (P<.01) ADG (1325 and 1325 vs 905 g/d), ADFI (4630 and 4637 vs 2760 g/d), feed:gain ratio (3.53 and 3.52 vs 3.11), and heavier carcasses (125.1 and 124.4 vs 113.2 kg; P<.01) with lower lean meat content (52.21 and 52.09 vs 55.83 %; P<.01). The results point out how immunocastrated pigs fed ad lib. showed similar rearing and slaughtering performance regardless of the inclusion of M and D in the diet

Therapeutic targeting of the PLK1-PRC1-axis triggers cell death in genomically silent childhood cancer

Chromosomal instability (CIN) is a hallmark of cancer1. Yet, many childhood cancers, such as Ewing sarcoma (EwS), feature remarkably ‘silent’ genomes with minimal CIN2. Here, we show in the EwS model how uncoupling of mitosis and cytokinesis via targeting protein regulator of cytokinesis 1 (PRC1) or its activating polo-like kinase 1 (PLK1) can be employed to induce fatal genomic instability and tumor regression. We find that the EwS-specific oncogenic transcription factor EWSR1-FLI1 hijacks PRC1, which physiologically safeguards controlled cell division, through binding to a proximal enhancer-like GGAA-microsatellite, thereby promoting tumor growth and poor clinical outcome. Via integration of transcriptome-profiling and functional in vitro and in vivo experiments including CRISPR-mediated enhancer editing, we discover that high PRC1 expression creates a therapeutic vulnerability toward PLK1 inhibition that can repress even chemo-resistant EwS cells by triggering mitotic catastrophe. Collectively, our results exemplify how aberrant PRC1 activation by a dominant oncogene can confer malignancy but provide opportunities for targeted therapy, and identify PRC1 expression as an important determinant to predict the efficacy of PLK1 inhibitors being used in clinical trials

Why women do not ask for information on preconception health? A qualitative study.

BACKGROUND: Preconception care involves health promotion to reduce risk factors that might affect women and couples of childbearing age. The risk factors of adverse reproductive outcomes include recognized genetic diseases in the family or the individual, previous congenital diseases, miscarriage, prematurity, fetal growth restriction, infertility, chronic maternal diseases, lifestyle, and occupational or environmental factors. Effective preconception care involves a range of preventive, therapeutic and behavioural interventions. Although in Italy there are national preconception care recommendations concerning the general population, they are usually encouraged informally and only for single risk factors. At present there is increasing interest in offering a global intervention in this field. The aim of this study was to investigate attitudes and behaviours of Italian women of childbearing age and healthcare professionals regarding preconception health. METHODS: We conducted a qualitative study among women of childbearing age and healthcare professionals between February 2014 and February 2015. Five focus groups were held: 2 with non-pregnant women aged 22 to 44 years and 3 with healthcare professionals. Discussion topics included women's questions about preconception health, worries and barriers regarding preconception care interventions, attitudes and behaviours of women and healthcare professionals towards preconception health, women's information sources. In the analysis of the focus groups priority was given to what was said by the women, supplemented by information from the healthcare professionals' focus groups. RESULTS: Fourteen women of childbearing age (8 nulliparae and 6 multiparae) and 12 healthcare professionals (3 nurses, 4 midwives, 5 doctors) participated in the focus groups. The results indicate the presence of many barriers and a lack of awareness of preconception health relating to women, healthcare professionals and policies. Women's knowledge and attitudes towards primary preconception care information are described. The main reference source of information in this field for Italian women seems to be their obstetric-gynaecologist. CONCLUSIONS: The study indicates that several barriers influence preconception care in Italy. Moreover, a lack of awareness of preconception health and care among Italian women of childbearing age and healthcare professionals emerges. The findings might contribute to strategies for the implementation of preconception care guidelines

Why women do not ask for information on preconception health? A qualitative study.

BACKGROUND: Preconception care involves health promotion to reduce risk factors that might affect women and couples of childbearing age. The risk factors of adverse reproductive outcomes include recognized genetic diseases in the family or the individual, previous congenital diseases, miscarriage, prematurity, fetal growth restriction, infertility, chronic maternal diseases, lifestyle, and occupational or environmental factors. Effective preconception care involves a range of preventive, therapeutic and behavioural interventions. Although in Italy there are national preconception care recommendations concerning the general population, they are usually encouraged informally and only for single risk factors. At present there is increasing interest in offering a global intervention in this field. The aim of this study was to investigate attitudes and behaviours of Italian women of childbearing age and healthcare professionals regarding preconception health. METHODS: We conducted a qualitative study among women of childbearing age and healthcare professionals between February 2014 and February 2015. Five focus groups were held: 2 with non-pregnant women aged 22 to 44 years and 3 with healthcare professionals. Discussion topics included women's questions about preconception health, worries and barriers regarding preconception care interventions, attitudes and behaviours of women and healthcare professionals towards preconception health, women's information sources. In the analysis of the focus groups priority was given to what was said by the women, supplemented by information from the healthcare professionals' focus groups. RESULTS: Fourteen women of childbearing age (8 nulliparae and 6 multiparae) and 12 healthcare professionals (3 nurses, 4 midwives, 5 doctors) participated in the focus groups. The results indicate the presence of many barriers and a lack of awareness of preconception health relating to women, healthcare professionals and policies. Women's knowledge and attitudes towards primary preconception care information are described. The main reference source of information in this field for Italian women seems to be their obstetric-gynaecologist. CONCLUSIONS: The study indicates that several barriers influence preconception care in Italy. Moreover, a lack of awareness of preconception health and care among Italian women of childbearing age and healthcare professionals emerges. The findings might contribute to strategies for the implementation of preconception care guidelines

Ryanodine receptor 1 (RYR1) mutations in two patients with tubular aggregate myopathy

Two likely causative mutations in the RYR1 gene were identified in two patients with myopathy with tubular aggregates, but no evidence of cores or core-like pathology on muscle biopsy. These patients were clinically evaluated and underwent routine laboratory investigations, electrophysiologic tests, muscle biopsy and muscle magnetic resonance imaging (MRI). They reported stiffness of the muscles following sustained activity or cold exposure and had serum creatine kinase elevation. The identified RYR1 mutations (p.Thr2206Met or p.Gly2434Arg, in patient 1 and patient 2, respectively) were previously identified in individuals with malignant hyperthermia susceptibility and are reported as causative according to the European Malignant Hyperthermia Group rules. To our knowledge, these data represent the first identification of causative mutations in the RYR1 gene in patients with tubular aggregate myopathy and extend the spectrum of histological alterations caused by mutation in the RYR1 gene

Finding the Optimal Balance between Over and Under Approximation of Models Inferred from Execution Logs

Models inferred from execution traces (logs) may admit more behaviours than those possible in the real system (over-approximation) or may exclude behaviours that can indeed occur in the real system (under-approximation). Both problems negatively affect model based testing. In fact, over-approximation results in infeasible test cases, i.e., test cases that cannot be activated by any input data. Under-approximation results in missing test cases, i.e., system behaviours that are not represented in the model are also never tested. In this paper we balance over- and under-approximation of inferred models by resorting to multi-objective optimization achieved by means of two search-based algorithms: A multi-objective Genetic Algorithm (GA) and the NSGA-II. We report the results on two open-source web applications and compare the multi-objective optimization to the state-of-the-art KLFA tool. We show that it is possible to identify regions in the Pareto front that contain models which violate fewer application constraints and have a higher bug detection ratio. The Pareto fronts generated by the multi-objective GA contain a region where models violate on average 2% of an application's constraints, compared to 2.8% for NSGA-II and 28.3% for the KLFA models. Similarly, it is possible to identify a region on the Pareto front where the multi-objective GA inferred models have an average bug detection ratio of 110: 3 and the NSGA-II inferred models have an average bug detection ratio of 101: 6. This compares to a bug detection ratio of 310928: 13 for the KLFA tool. © 2012 IEEE