An experimental and theoretical study of stereoselectivity of furan-maleic anhydride and furan-maleimide Diels-Alder reactions

The stereoselectivity of the reaction of furan (1) with maleic anhydride (2) and maleimide (3) was studied experimentally and theoretically. Although the two reactions are highly similar with regard to their preference for endo and exo steroisomers, no

Factory and site tests on the 25 MW GTO power amplifier for the control of the JET plasma vertical position

SIGLEAvailable from British Library Document Supply Centre- DSC:4672.262(JET-P--93/31) / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Design, construction and operation of the 66 kA life test facility for the ITER magnet protection vacuum circuit breaker

The characteristics of the 66 kA test facility built up at the Consorzio RFX are presented and discussed together with the results of interruption and life tests performed on a new vacuum circuit breaker intended to fulfil possible ITER requirements for the Fast Discharge Unit

Status of ITER coil power supply design

This paper focuses on the recent modifications of the Coil Power Supply and Distribution System (CPSDS) to adapt it to the revised magnet configuration and plasma scenarios. Updated performance of the main components (AC/DC converters, switching networks and discharge circuits) are presented. The layout of the equipment is described with emphasis on the revision made to meet the new seismic requirements and to reduce the cost of busbars

ΔNp63α expression induces loss of cell adhesion in triple-negative breast cancer cells

Background: p63, a member of the p53 protein family, plays key roles in epithelial development and carcinogenesis. In breast cancer, p63 expression has been found predominantly in basal-A (epithelial-type) triple-negative breast carcinomas (TNBC). To investigate the functional role of p63 in basal-A TNBC, we created MDA-MB-468 cell lines with inducible expression of the two major N-terminal p63 isoforms, TAp63α and ΔNp63α. Results: TAp63α did not have significant effect on gene expression profile and cell phenotype, whilst the main effect of ΔNp63α was reduction of cell adhesion. Gene expression profiling revealed genes involved in cell adhesion and migration whose expression relies on overexpression of ΔNp63α. Reduced cell adhesion also led to decreased cell proliferation in vitro and in vivo. Similar data were obtained in another basal-A cell line, BT-20, but not in BT-549 basal-B (mesenchymal-like) TNBC cells. Conclusions: In basal-A TNBC cells, ΔNp63α has much stronger effects on gene expression than TAp63α. Although p63 is mentioned mostly in connection with breast cell differentiation and stem cell regulation, we showed that a major effect of p63 is regulation of cell adhesion, a process important in metastasis and invasion of tumour cells. That this effect is not seen in mesenchymal-type TNBC cells suggests lineage-dependent functions, mirroring the expression of ΔNp63α in primary human breast cancers. © 2016 The Author(s)